This paper describes a simple technique for staining of flatworms using lactophenol cotton blue (LPCB). The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies.
HENEDI, A., & EL-AZAZY, O. (2013). A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN. Journal of the Egyptian Society of Parasitology, 43(2), 419-423. doi: 10.21608/jesp.2013.94819
MLA
ADAWIA A.M. HENEDI; OSAMA M.E. EL-AZAZY. "A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN". Journal of the Egyptian Society of Parasitology, 43, 2, 2013, 419-423. doi: 10.21608/jesp.2013.94819
HARVARD
HENEDI, A., EL-AZAZY, O. (2013). 'A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN', Journal of the Egyptian Society of Parasitology, 43(2), pp. 419-423. doi: 10.21608/jesp.2013.94819
VANCOUVER
HENEDI, A., EL-AZAZY, O. A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN. Journal of the Egyptian Society of Parasitology, 2013; 43(2): 419-423. doi: 10.21608/jesp.2013.94819