A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN

Document Type : Original Article

Authors

Veterinary Laboratories, Public Authority of Agriculture Affairs and Fish Resources, P. O. Box 21422, Safat 13075, Kuwait.

Abstract

This paper describes a simple technique for staining of flatworms using lactophenol cotton blue (LPCB). The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies.

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