SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES

ELSAGHEIR, ASMAA M.A.; ABD ELLAH, OSAMA H.; SAYED, FATMA G.; MONIB, MOHAMMED E.M.; ABDEL-KADER, MOHAMMAD S.

doi:10.21608/jesp.2015.89911

SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES

Document Type : Original Article

Authors

¹ Department of Medical Parasitology, Faculty of Medicine, South Valley University, Egypt.

² Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

³ Department of Urology, Faculty of Medicine, South Valley University, Egypt.

10.21608/jesp.2015.89911

Abstract

Fasciola and Schistosoma spp. are digenetic trematodes that have a major detrimental impact on human health worldwide. It is not unusual to find common molecules among parasites of different species, genera, or phyla. In this study Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify the common proteins of adult Fasciola and Schistosoma spp. Adult Fasciola spp, were collected from the bile ducts of naturally infected
cattle and sheep. Schistosoma mansoni and S. haematobium adults surface antigens were prepared in Theodor Bilharz Research Institute were used. Results showed sharing bands between all worms, which have the same molecular weight of 36 kDa. On the other hand, band with molecular weight 41 kDa was sharing between them except F. hepatica. Bands at 48 and 170 kDa were sharing between all species of Fasciola spp and Schistosoma mansoni.

Keywords