COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE

ISMAIL, MOUSA A.M.; MOUSA, WAHED MOHAMMED ALI; ABU-SAREA, ENAS YAHIA; BASYOUNI, MAHA M. A.; MOHAMMED, SAMAH SAYED

doi:10.21608/jesp.2016.88954

COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE

Document Type : Original Article

Authors

¹ Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.

² Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Egypt.

³ Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Egypt.

10.21608/jesp.2016.88954

Abstract

This study compared PCR and Western blot techniques in diagnosis of schistosomiasis mansoni. Forty Swiss albino mice were used, thirty two mice were infected with cercariae of S. mansoni and eight mice were kept uninfected which were used as a control. Blood was obtained from four infected mice weekly beginning from the 1st week to the 8th week post infection. The study found that PCR was positive from the first week post infection, while Western blot technique was positive from the second week post infection. Thus, PCR diagnosed schistosomiasis mansoni earlier than Western blot technique, but both were able to diagnose.

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