ALTERNATIVE STAINING OF FASCIOLA GIGANTICA USING NATURAL HERBAL DYES

ALY, MARWA E.; DYAB, AHMED K.; YONES, DOAA A.; MOHAMED, YASSER M.; BAYOUMI, SOAAD A.; ELOSSILY, NAHED A.

doi:10.21608/jesp.2020.88809

ALTERNATIVE STAINING OF FASCIOLA GIGANTICA USING NATURAL HERBAL DYES

Document Type : Original Article

Authors

¹ Animal Health Research Institute, Faculty of Medicine, Assuit University, Egypt.

² Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.

³ Department of Pharmacognosy, Faculty of Pharmacy, Assuit University, Egypt

10.21608/jesp.2020.88809

Abstract

The Aqueous extracts of red beet, China rose, henna and acid carmine stains were used to stain the adult worms of F. gigantca. The stained flukes showed varying degrees of pigmentation with well-defined external and internal structure. Oral and ventral sucker, muscular pharynx, oesophagus, intestinal ceca and cirrus sac took rose to light cherry color in aqueous extract of china rose, orange to brown color in aqueous extract of sugar beet, beige to pale brown color in aqueous extract of henna while they took deep red color in carmine stain with best quality achieved by china rose aqueous extract. The ovary, testes and longitudinal excretory canal/pore took rose to cherry color in aqueous extract of China rose, pale brown color in aqueous extracts of both sugar beet and henna while they acquired red to pink color in acid carmine stain. Aqueous extracts of sugar beet exhibited little better quality in revealing characteristics of vitelline ducts and
vitelline glands compared to the aqueous extract of China rose, henna and carmine stain. Uterus stained brown in both sugar beet aqueous extracts and carmine stain while it took yellow color when stained with China rose and henna. Eggs inside uterus took light yellow color in all tested aqueous extracts. Ootype/mehli gland and yolk reservoir of F. gigantica took brown color in aqueous extracts of red beet, China rose and henna, red to pink color in carmine stain. Spines
covering the cuticle appeared rose color in aqueous extract of china rose, pale brown color in aqueous extract of sugar beet extract and henna, and pale red color in carmine stain.

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