Liver breast cancer represents a major health problem worldwide. The present study evaluated the anti-cancer properties of Shrimp derived chitosan on human liver (HepG-2) and breast cancer (MCF7) cell lines. The cytotoxicity of chitosan was determined using MTT assay. Chitosan showed an antiproliferative and cytotoxic effect against (HEPG-2) and (MCF7) cancer cell lines in a dose and cell type dependent manner. The IC50 value of chitosan was 600μg/ml and 348μg/ml for treated HepG-2 and MCF-7 respectively. Anticancer potential of chitosan was monitored via evaluation of cell cycle profile and pro- and anti-apoptotic genes. Cell cycle arrest profile was cell type dependent and occurred insignificantly (P>0.05) during S and G2-M phases in case of HepG2 cells, while it was significantly elevated during G2-M phase in case of MCF-7 treatment. Treated cell lines showed a significant apoptotic % during the Pre-G1 phase (P<0.05). ER and HER-2 as specific markers for MCF-7 and BCL-2 in treated HEPG-2 cells were significantly down regulated (P<0.05), while, p53 was significantly up regulated (P<0.05).
GHALY, C., BAKAAR, A., & MOHAMED, A. (2018). IN VITRO ASSESSMENT OF ANTICANCER ACTIVITY OF SHRIMP DERIVED CHITOSAN AND RELATED APOPTOTIC PROFILE ALTERATION. Journal of the Egyptian Society of Parasitology, 48(2), 379-388. doi: 10.12816/0050445
MLA
CHRISTINA ATEF FAWZY GHALY; ASHRAF BAKAAR; ALY FAHMY MOHAMED. "IN VITRO ASSESSMENT OF ANTICANCER ACTIVITY OF SHRIMP DERIVED CHITOSAN AND RELATED APOPTOTIC PROFILE ALTERATION". Journal of the Egyptian Society of Parasitology, 48, 2, 2018, 379-388. doi: 10.12816/0050445
HARVARD
GHALY, C., BAKAAR, A., MOHAMED, A. (2018). 'IN VITRO ASSESSMENT OF ANTICANCER ACTIVITY OF SHRIMP DERIVED CHITOSAN AND RELATED APOPTOTIC PROFILE ALTERATION', Journal of the Egyptian Society of Parasitology, 48(2), pp. 379-388. doi: 10.12816/0050445
VANCOUVER
GHALY, C., BAKAAR, A., MOHAMED, A. IN VITRO ASSESSMENT OF ANTICANCER ACTIVITY OF SHRIMP DERIVED CHITOSAN AND RELATED APOPTOTIC PROFILE ALTERATION. Journal of the Egyptian Society of Parasitology, 2018; 48(2): 379-388. doi: 10.12816/0050445