CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT

ABDEL-RAHMAN, SALMA M.; HASSAN, TASNEEM M.; BAKIR, HANAA Y.; OTHMAN, RAGAA A.; KHALIFA, MERVAT M.; OMRAN, EMAN KH.

doi:10.21608/jesp.2025.447404

CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT

Document Type : Original Article

Authors

¹ Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.

² Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt

³ Department of Medical Parasitology Faculty of Medicine, Sohag University, Egypt

10.21608/jesp.2025.447404

Abstract

Intestinal capillariasis is an emerging zoonotic disease. It can lead to life-threatening protein
losing enteropathy if not diagnosed early and treated promptly. This study clarified the antige-
nic profiles and immunogenicity of C. philippinensis from adult, egg, coproantigen, and fish
Capillaria spp. adult antigen using SDS-PAGE and Western Blot (WB) techniques against C.
philippinensis sera. The electrophoretic profiles showed that most proteins were common in all
samples at 25.6, 42, 57, & 70 kDa, but some were stage-specific. Immunoblot analysis showed
that patient sera recognized and reacted with all examined stages. This indicated that antigenic
components of different stages of C. philippinensis, and the common components among all
life stages improved parasite immunodiagnosis. Also, the results showed that human sera from
patients with intestinal capillariasis recognized the antigenic pattern of fish Capillaria spp.,
indicated that potential cross-reactivity could develop more accessible and cost-effective diag-
nostic tools, mainly in regions where C. philippinensis-specific antigens in non-endemic areas.

Keywords