Blastocystis is an anaerobic parasite that was identified as the most common eukaryotic organism reported in human fecal samples. Data on treatment options signifies that efficacious antimicrobial eradication of Blastocystis spp. is still far from straight forward. As well as Metronidazole, has shown some resistance. The study evaluated chitosan and chitosan Nano particles against Blastocystis in vitro. Blastocystis species in stool samples were cultured. Culture tubes were divided into three groups: Chitosan, Chitosan Nano particles and Metronidazole (reference drug), and positive control. Different criteria were used to evaluate the tested drugs on Blastocystis and assessing the viability before and after treatment by the use of Neutral red stain and recording structural changes. Also, TEMand the single cell gel electrophoresis (SCGE)/comet assay were used. The results showed a direct relation between increase in the concentration of Chitosan and Chitosan nano-particles and exposure time and increase in the mortality rates. TEM showed morphological damage when exposed to tested drug, Comet assay showed direct relationship between the increase in the dose of Chitosan Nano particles NPs and the degree of DNA genotoxic destruction.
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., & RIZK, E. (2024). IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES. Journal of the Egyptian Society of Parasitology, 54(2), 283-294. doi: 10.21608/jesp.2024.373531
MLA
SARAH IBRAHIM MOHAMED EZZ EL DINE; ABEER SAID ABD EL-GHANY AL-ANTABLY; MOHAMED M. EL BAHY; DINA MOHAMED HAMDY EL AKAD; ENAS M. A. RIZK. "IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES". Journal of the Egyptian Society of Parasitology, 54, 2, 2024, 283-294. doi: 10.21608/jesp.2024.373531
HARVARD
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., RIZK, E. (2024). 'IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES', Journal of the Egyptian Society of Parasitology, 54(2), pp. 283-294. doi: 10.21608/jesp.2024.373531
VANCOUVER
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., RIZK, E. IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES. Journal of the Egyptian Society of Parasitology, 2024; 54(2): 283-294. doi: 10.21608/jesp.2024.373531
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