GENISTEIN FOR EXPERIMENTAL MURINE TRICHINELLOSIS: A NOVEL THERAPEUTIC INSIGHT WITH EVALUATION OF IMMUNOLOGICAL AND IMMUNOHISTOCHEMICAL PARAMETERS

Document Type : Original Article

Authors

1 Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

2 Department of Pathology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Abstract

Herbal remedies have been studied as alternative or adjuvant treatment options against parasitic infections.
Treating trichinellosis with albendazole (ABZ) showed many drawbacks despite its efficacy.
This study assessed the effectiveness of genistein (Soybean extract), either with or without ABZ, in
treating experimental murine trichinellosis and evaluated the regulatory mechanisms. One hundred
Swiss albino laboratory-bred mice were categorized into five groups: G1 uninfected and untreated
mice, G2 infected untreated mice, G3 infected and ABZ treated, G4; infected and genistein treated, G5
infected and ABZ &genistein treated. Each group was divided according to the scarification time (7-
or 40-days post-infection (dpi), respectively). The treatment efficacy was evaluated parasitological,
histopathological, immunological, immunohistochemical (GATA3, glutathione peroxidase 1 (GPX1),
and caspase 3) methods and by SEM. Combination of genistein & ABZ in treating trichinellosis showed
the best drug efficacy, with reduction of adults and larvae count at 96.73% & 81.56 %, respectively.
They showed the highest degree in amelioration of histopathological changes and reduction of inflammation
parallel to genistein-treated ones with significant reduction of GATA3 & caspase 3 expressions,
but significant elevation of GPX1 expression as well as highest degeneration of adults and larvae
by SEM.

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