BLASTOCYSTIS SUBTYPE 3 AMONG ADOLESCENTS WITH GASTROINTESTINAL SYMPTOMS IN FAYOUM GOVERNORATE, EGYPT

Document Type : Original Article

Authors

1 Department of Medical Parasitology, Faculty of Medicine, Fayoum University

2 Department of Public Health, Damanhour National Medical Institute, Damanhour, Egypt

Abstract

Blastocystis is a common polymorphic protozoan infecting human intestine with a zoonotic
potential. Despite the unclear pathogenicity, its existence may be linked to a wide range of
gastrointestinal disorders. The present cross sectional study was conducted to analyze Blastocystis
subtypes among 110 adolescents suffering from disturbing GIT symptoms in stool samples.
Positive samples (21 (19.1%)) by microscopy were subjected to DNA isolation and subtyped
by Sequence Tagged Site (STS)-PCR analysis, which successfully subtyped 10 (62.5%)
of 16 isolates. The dominant Blastocystis subtype was ST3 in 6 (40%) samples followed by 3
(30%) for ST2, but one sample (10%) was amplified for ST1. The results revealed significant
association between nausea and vomiting and Blastocystis infection. However, there was no
difference between the Blastocystis subtypes and demo-clinical characteristics.

Keywords

Journal of the Egyptian Society of Parasitology
Volume 53, Issue 3
December 2023
Pages 467-474

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