Cystic echinococcosis (CE) affects humans and animals worldwide. Zoonotic diagnoses depend on imaging primarily ultrasound and immunodiagnostic techniques, which are costeffective tools for epidemiological studies. The current study investigated the utilization of hydrated cyst fluids (HCF) as diagnostic antigens, which were collected from various intermediate hosts. Sixteen crude HCF samples from humans, sheep, gerbils, horses, and cows were evaluated for their reactivity with human sera using an enzyme-linked immunosorbent assay (ELISA). The Bradford protein assay was used to assess the total protein concentration of each sample of cyst fluid. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate the protein contained in the HCF of the samples. Immunoblotting was used to carry out antigen recognition. The HCF of the several intermediate hosts varied in their reactivity, according to ELISA results and this variation was unrelated to the protein content in the HCF samples. HCF samples' SDS-PAGE profiles revealed variance in several proteins and existence of 12 distinct protein bands ranging in size from 8 to 148 KDa. There were 98 & 64 KDa proteins found in all HCF samples. Bovine serum albumin was the 98KDa protein. In comparison, 19% of the fluid samples included an 8KDa protein indicative by AgB, while 50% of HCF samples contained a 50KDa protein. DS-PAGE profiles' proteins didn't show Ag5. The 64KDa protein was detected with potential for application in the diagnosis of human CE, according to immunoblot results.
ABU JABEL, O., & ROGAN, M. (2023). INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE. Journal of the Egyptian Society of Parasitology, 53(1), 141-150. doi: 10.21608/jesp.2023.297360
MLA
OMYMA M. ABU JABEL; MICHAEL T. ROGAN. "INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE". Journal of the Egyptian Society of Parasitology, 53, 1, 2023, 141-150. doi: 10.21608/jesp.2023.297360
HARVARD
ABU JABEL, O., ROGAN, M. (2023). 'INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE', Journal of the Egyptian Society of Parasitology, 53(1), pp. 141-150. doi: 10.21608/jesp.2023.297360
VANCOUVER
ABU JABEL, O., ROGAN, M. INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE. Journal of the Egyptian Society of Parasitology, 2023; 53(1): 141-150. doi: 10.21608/jesp.2023.297360
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