TOXIC EFFECTS OF FOUR PLANT OILS ON TROGODERMA GRANARIUM EVERTS AND PHEROMONE RESPONSE

Authors

1 Department of Entomology, Faculty of Science, Banha University

2 Plant Protection Research Institute, Agricultural Research Center, Dokki 12619

3 Department of Entomology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt

Abstract

This study evaluated the insecticidal bioassays of four known plant oils against the 4th larval instar
of the khapra beetle, Trogoderma granarium (Everts). Mortality% increased with increased
concentrations of oils and exposure time LC25 & LC50 showed the efficacy of cinnamon oils, followed
by black seed, lemon, and then camphor oils. They induced biochemical changes on T.
granarium adults with LC50 compared to control, which were tracked by analysis of carbohydrate-
hydrolysis enzymes to measure phosphatase enzymes changes post-treatment. Acid phosphatase
enzyme was significantly decreased when treated with black seed, lemon, and camphor oils,
but highly increased with cinnamon oil compared to control. Alkaline phosphatase was decreased
significantly post-treated with black seed and lemon oils, but increased significantly after
camphor and cinnamon oils treatment compared to control. Trehalase enzyme was significantly
decreased, but invertase and amylase decreased significantly except in cinnamon oil, which increased
significantly compared to control. All oils showed significantly less activity in acetylcholinesterase,
glutathione S-transferase, and cytochrome P-450 monooxygenase compared to control.
When the 4th larval instar was treated with LC25 of cinnamon oil (0.91%), male perception
of sex pheromone and female pheromone production were impacted. Pheromone production and
response were much higher in untreated beetles than in treated ones.

Keywords

Journal of the Egyptian Society of Parasitology
Volume 53, Issue 1
April 2023
Pages 123-133

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