CRYPTOSPORIDIOSIS: MOLECULAR ANALYSIS, RISK FACTORS AND SEASONAL ABUNDANCE IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS, KAFRELSHEIKH UNIVERSITY HOSPITALS

Document Type : Original Article

Authors

1 Departments of Medical Parasitology, Faculty of Medicine, Kafrelsheikh University

2 Departments of Medical Parasitology, Faculty of Medicine, Cairo University

3 Departments of Internal Medicine, Faculty of Medicine, Kafrelsheikh University

Abstract

This study detected of the molecular Cryptosporidium species in immunocompetent and immunocompromised
adults with diarrhea attended Kafrelsheikh University Hospitals and to assess
risk factors. Stool samples from 200 immunocompetent & 200 immunocompromised were
examined using acid fast (AF) stain and PCR/restriction fragment length polymorphism (RFLP)
targeting small subunit (SSU) rRNA gene. Cryptosporidium PCR amplified was 15(7.5%) in immunocompetent
and 23 (11.5%) immunocompromised, with significance difference as to age,
presence of mucous, abdominal pain (P<0.05). Cryptosporidium was more common in rural
areas in autumn and winter seasons. Two genotypes were C. hominis and C. parvum, but C.
hominis was 60% in immunocompetent and 82.6% in immunocompromised.

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