Toxoplasma gondii (T. gondii) protozoan invades any nucleated cell with a special predilection to reticuloendothelial cells. Liver is severely affected during acute toxoplasmosis. Promisingly, fisetin (FIS) flavonoid was proved to have antioxidant, anti-inflammatory, anti-cancerous, and anti-protozoal effects. This study was designed to evaluate the anti-Toxoplasma effect and the hepatoprotective potential of FIS in vivo. Mice were infected with the virulent RH strain of T. gondii in a dose of 1x103tachyzoites/mouse. Mice were divided into seven equal groups: G1: healthy control, G2: infected control, G3: Pyrimethamine® (PYR)/sulfadiazine (SDZ)), G4: oral-FIS, G5: oral-FIS+ PYR treated, G6: intraperitoneal (I.P) FIS, and G7: I.P FIS+PYR treated. Survival rate was estimated, and tachyzoites were counted in liver impression smears. Liver enzymes and C reactive protein (CRP) were measured in mice sera. Liver tissues were examined for inflammatory mediators; cyclooxygenase 2 (COX- 2), interleukin (IL) -10, IL-12, IL- , oxidative mediators; inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO), and malondialdehyde (MDA) FIS showed a moderate effect on parasite survival and tachyzoites count, with an excellent effect in reducing serum liver enzymes activity and proinflammatory mediators. Combined (FIS+PYR) showed the best anti-parasitic and hepatoprotective actions. Its administration I.P. gave significant cure compared to oral route. FIS when given I.P than oral FIS had a good anti-inflammatory and antioxidant effect, decreased inflammatory cytokines and improved hepatic pathology of acute toxoplasmosis. Combined FIS/PYR was the best drug regimen.
ELMEHY, D., SALAMA, A., SOLIMAN, N., ELKHOLY, R., TAHOON, D., MADY, R., & GAMEA, G. (2021). AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS. Journal of the Egyptian Society of Parasitology, 51(1), 79-88. doi: 10.21608/jesp.2021.165943
MLA
DALIA A. ELMEHY; AMINA M. SALAMA; NEMA A. SOLIMAN; REEM A. ELKHOLY; DINA M. TAHOON; RASHA F.Department of Medical Parasitology MADY; GHADA A. GAMEA. "AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS". Journal of the Egyptian Society of Parasitology, 51, 1, 2021, 79-88. doi: 10.21608/jesp.2021.165943
HARVARD
ELMEHY, D., SALAMA, A., SOLIMAN, N., ELKHOLY, R., TAHOON, D., MADY, R., GAMEA, G. (2021). 'AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS', Journal of the Egyptian Society of Parasitology, 51(1), pp. 79-88. doi: 10.21608/jesp.2021.165943
VANCOUVER
ELMEHY, D., SALAMA, A., SOLIMAN, N., ELKHOLY, R., TAHOON, D., MADY, R., GAMEA, G. AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS. Journal of the Egyptian Society of Parasitology, 2021; 51(1): 79-88. doi: 10.21608/jesp.2021.165943
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