SADEK, H., ABDEL-RAHMAN, S., BAKIR, H., ARAFA, M., AHMED, A., GABER, M. (2020). THE POTENTIAL CONVENTION OF GARLIC AND BLACK SEED DIFFERENT EXTRACTS AS AN EFFECTIVE TREATMENT OF CRYPTOSPORIDIUM SPP. : AN EXPERIMENTAL STUDY. Journal of the Egyptian Society of Parasitology, 50(3), 613-621. doi: 10.12816/jesp.2020.131094
HESHAM A. SADEK; SALMA M. ABDEL-RAHMAN; HANNA Y. BAKIR; MOHSEN I. ARAFA; AHMED A. AHMED; MONA M. GABER. "THE POTENTIAL CONVENTION OF GARLIC AND BLACK SEED DIFFERENT EXTRACTS AS AN EFFECTIVE TREATMENT OF CRYPTOSPORIDIUM SPP. : AN EXPERIMENTAL STUDY". Journal of the Egyptian Society of Parasitology, 50, 3, 2020, 613-621. doi: 10.12816/jesp.2020.131094
SADEK, H., ABDEL-RAHMAN, S., BAKIR, H., ARAFA, M., AHMED, A., GABER, M. (2020). 'THE POTENTIAL CONVENTION OF GARLIC AND BLACK SEED DIFFERENT EXTRACTS AS AN EFFECTIVE TREATMENT OF CRYPTOSPORIDIUM SPP. : AN EXPERIMENTAL STUDY', Journal of the Egyptian Society of Parasitology, 50(3), pp. 613-621. doi: 10.12816/jesp.2020.131094
SADEK, H., ABDEL-RAHMAN, S., BAKIR, H., ARAFA, M., AHMED, A., GABER, M. THE POTENTIAL CONVENTION OF GARLIC AND BLACK SEED DIFFERENT EXTRACTS AS AN EFFECTIVE TREATMENT OF CRYPTOSPORIDIUM SPP. : AN EXPERIMENTAL STUDY. Journal of the Egyptian Society of Parasitology, 2020; 50(3): 613-621. doi: 10.12816/jesp.2020.131094
THE POTENTIAL CONVENTION OF GARLIC AND BLACK SEED DIFFERENT EXTRACTS AS AN EFFECTIVE TREATMENT OF CRYPTOSPORIDIUM SPP. : AN EXPERIMENTAL STUDY
2Animal Health Research Institute, Assiut, Department of Medical Parasitology
3Faculty of Medicine, and Department of Pharmacognosy
Abstract
The study investigated the effect of garlic (Allium sativum) and black seed (Nigella sativa) extracts products as treatment of Cryptosporidium infection in experimental mice in comparison to Nitazoxanide (NTZ). Forty-eight male Swiss Albino mice divided into 8 experimental conditions; G1-8, the first seven groups received 1.5×105 Cryptosporidium spp. oocysts on 1st infection day. The first group included mice treated with garlic juice; in G2, G4 & G5, mice were treated with black seed oil, alcoholic extract of black seed and alcoholic extract of black ariel parts respectively. In G3 mice were treated with garlic & black seed oil mixture, while G6 mice treated were with Nitaxanoide. Besides G7 were infected untreated (+ ve control); and G8 were neitherinfected nor treated (– ve control); From 2nd day post-infection, pooled fecal samples of each group were collected daily to assess each extract efficacy as compared with NTZ and both +ve and –ve controls by calculating oocyst excretion pattern. The comparison between number of oocysts shedding rates among different treatment groups showed that the efficacy of NTZ was the highest (76.1%) followed by N. sativa oil (75.8%) followed by mixture of garlic and black seed oil (75.4%), alcoholic black seed extract (75.1%); alcoholic arial extract (74.7%) and the lowest was garlic juice (73.1%). Although both A. sativum and all N. sativa extract products showed a great degree of efficacy in reducing Cryptosporidium spp. oocysts excretions in the experimental animals compared to positive control, but without significance differences between them and preparation of both garlic, black seed and mixture with NTZ.
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