MADBOULY, N., MOHAMED, D., BAUIOMY, I., EL AMIR, A. (2020). IMMUNOLOCALIZATION OF EXCRETORY/SECRETORY ANTIGENS EXPRESSED ON DIFFERENT LIFE CYCLE STAGES AND IN DIFFERENT ORGANS OF FASCIOLA GIGANTICA INFECTED CATTLE. Journal of the Egyptian Society of Parasitology, 50(3), 580-589. doi: 10.12816/jesp.2020.131090
NEVEEN A. MADBOULY; DOAA A. MOHAMED; IBRAHEEM R. BAUIOMY; AZZA M. EL AMIR. "IMMUNOLOCALIZATION OF EXCRETORY/SECRETORY ANTIGENS EXPRESSED ON DIFFERENT LIFE CYCLE STAGES AND IN DIFFERENT ORGANS OF FASCIOLA GIGANTICA INFECTED CATTLE". Journal of the Egyptian Society of Parasitology, 50, 3, 2020, 580-589. doi: 10.12816/jesp.2020.131090
MADBOULY, N., MOHAMED, D., BAUIOMY, I., EL AMIR, A. (2020). 'IMMUNOLOCALIZATION OF EXCRETORY/SECRETORY ANTIGENS EXPRESSED ON DIFFERENT LIFE CYCLE STAGES AND IN DIFFERENT ORGANS OF FASCIOLA GIGANTICA INFECTED CATTLE', Journal of the Egyptian Society of Parasitology, 50(3), pp. 580-589. doi: 10.12816/jesp.2020.131090
MADBOULY, N., MOHAMED, D., BAUIOMY, I., EL AMIR, A. IMMUNOLOCALIZATION OF EXCRETORY/SECRETORY ANTIGENS EXPRESSED ON DIFFERENT LIFE CYCLE STAGES AND IN DIFFERENT ORGANS OF FASCIOLA GIGANTICA INFECTED CATTLE. Journal of the Egyptian Society of Parasitology, 2020; 50(3): 580-589. doi: 10.12816/jesp.2020.131090
IMMUNOLOCALIZATION OF EXCRETORY/SECRETORY ANTIGENS EXPRESSED ON DIFFERENT LIFE CYCLE STAGES AND IN DIFFERENT ORGANS OF FASCIOLA GIGANTICA INFECTED CATTLE
1Department of Zoology, Faculty of Science, Cairo University
2Department of Parasitology, Theodore Bilharz Research Institute
Abstract
In this study, polyclonal antibodies (pAb) were prepared against Fasciola gigantica excretory/ secretory (E/S) products and purified to demonstrate the highly reactive epitopes on the different life cycle stages of F. gigantica and on blood cells and different organs of naturally infected cattle, as well as to screen internal organs for localization of E/S antigens to find out a suitable method for diagnosing the disease as early as possible. Using anti-F. gigantica E/S IgG pAb, immunostaining of different life cycle stages of F. gigantica detected strong positive reactions in all stages except eggs. The reactions were along tegumental surface, gut region of cercariae, miracidiae, metacercariae and tail of cercariae. Besides, the tegument, spine surface, muscularis and gut region of the adults were also positive. Immunoperoxidase localization of E/S products by anti-F. gigantica E/S IgG pAb showed that E/S antigens were located in many organs of naturally infected cattle. Examination of peripheral blood mononuclear cells (PBMNCs) represented a high positivity in the phagosomes and activated monocytes as well as in cytoplasm of lymphocytes. Compared to sections of normal uninfected hosts, liver sections of infected bovine hosts showed collapsed hepatocytes, thickened portal tract and a highly positive reaction in the cytoplasm of the liver cells. Target epitopes were located inside the splenic cells, mainly lymphocytes. In the gall bladder, positive peroxidase reactions were found in the mucosa and muscularis layers, and in the epithelial cells lining mucosal glands. A marked positivity was in other organs of the naturally infected animals, as in the epithelial layer lining the glomeruli and tubules of the kidney, in heart cardiac muscle fibres and in cells lining the alveoli of the lungs.
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