EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS

Document Type : Original Article

Authors

1 Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt

2 Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.

Abstract

Cryptosporidium is recognized globally as a major etiology of persistent and chronic diarrhea
especially in immunocompromised patients and children with significant morbidity and mortality
rates. Moreover, it is one of the most important pathogens causing waterborne outbreaks.
Hence, rapid diagnosis is crucial. In this study, we tried to determine the diagnostic performance
of Rida®Quick Cryptosporidium cassettes versus microscopy of the modified acid-fast
stained smear. Forty fecal samples were collected from immunocompromised patients complaining
of chronic diarrhea. All samples were examined by Rida®Quick Cryptosporidium cassettes
for copro-antigen detection, and microscopically using concentration techniques and
modified acid-fast staining. Cryptosporidium was detected in (45%) of immunocompromised
patients. The sensitivity, specificity, PPV, and NPV of Rida®Quick Cryptosporidium cassettes
were 88.9%, 95.5%, 94.1% & 91.3% respectively with an accuracy of 92.5%. There was no
cross-reactivity with other intestinal parasites. Rida®Quick Cryptosporidium cassettes provide
adequate sensitivity and specificity and give rapid results. Our results recommend its use as an
alternative test in certain situations especially when screening large populations is needed urgently
in a short time, as in outbreaks and epidemiological surveys.

Keywords

Journal of the Egyptian Society of Parasitology
Volume 50, Issue 3
December 2020
Pages 477-482

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