The Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801DIFFERENTIATION & HOMING OF BONE MARROW-DERIVED MESENCHYMAL STEM CELLS IN THE LIVER SCHISTOSOMA INFECTED MICE2492566812810.21608/jesp.2019.68128ENAMIRA A.SALEHDepartment of Parasitology, Faculty of Medicine, Zagazig University, Egypt.AYMAN A.SALEHDepartment of Animal Wealth
Development, Genetics & Genetic Engineering, Faculty of Veterinary Medicine,
Zagazig University, Egypt.ENAS F.ABDEL HAMEDDepartment of Parasitology, Faculty of Medicine, Zagazig University, Egypt.EMAN M.FAWZYDepartment of Parasitology, Faculty of Medicine, Zagazig University, Egypt.MARWA A.SALAMADepartment of Parasitology, Faculty of Medicine, Zagazig University, Egypt.Journal Article20200114Schistosomiasis is an endemic parasitic infective disease that remains widespread in several countries. This work aimed to evaluate the curative influence of bone marrow derived mesenchymal stem cells (BM-MSC) engraftment in liver of schistosomiasis chronic infected mice. 30 Swiss albino mice divided into 3 groups of 10 mice each. G1: normal healthy group, G2: infected with Schistosoma mansoni cercariae subcutaneously and G3: infected and transplanted with BM-MSC on their 8th week post infection by intravenous injection. All mice were sacrificed 4 weeks post-transplantation. Serum was collected for assessment of albumin, ALT & AST levels. Engraftment of BM-MSC was assessed by labeling with PKH26. Histopathological and ultrastructural studies were carried out. Expression of capase-3 & IL-1as a marker of apoptosis and inflammation respectively were measured by quantitative RT-PCR. Homing of transplanted BM-MSC within the injured liver was confirmed by fluorescent microscopic examination. MSC labeled with PKH26 were recovered in liver. Histopathological and electron microscopic examinations of liver showed differentiation of transplanted BM-MSC into hepatocyte-like cells. Liver function was improved confirmed by elevation of serum albumin and decrease of ALT & AST in G3. Real-time PCR revealed that BM-MSC inhibited capase-3 and suppressinterleukin-1 mRNA expression in damaged liver tissues. MSC-treated group had the least apoptosis cells and inflammatory cells. Transplantation of BM-MSCs has therapeutic anti-apoptotic anti-inflammatory mechanisms effect in chronic liver disease.https://jesp.journals.ekb.eg/article_68128_1b98a311bd81a7b1a23830785b62121f.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE ECHIS COLORATUS VENOM TOXICITY IN MICE2572646812910.21608/jesp.2019.68129ENANDREW R.GABRAFaculty of Biotechnology, October University for Modern science and Arts, 6th October,
Giza, Egypt.MOHAMEDMAGEDFaculty of Biotechnology, October University for Modern science and Arts, 6th October,
Giza, Egypt.LAMIAA S. EL-DINSHAKERVACSERA, Applied Research Department, Pilot plant unit, Agouza, Giza, Egypt.OSAMA A.ABBASDepartment of Radioisotopes, Egyptian Atomic Energy Authority.SAMAH G.MOHAMEDVACSERA, Applied Research Department, Pilot plant unit, Agouza, Giza, Egypt.Journal Article20200114Snake venoms are complex mixtures of proteins and non proteins components. This study evaluated the toxicity effect of LD50 of native Echis coloratus venom and the LD50 of dry venom irradiated with different doses of gamma radiation (2, 5 & 10 KGy) on Swiss albino mice. Measurement of some biochemical parameters and histological alterations in liver and kidney was investigated. The biochemical parameters showed highly significant increase (P< 0.001) in levels of ALT, AST, urea and CK in all groups compared to control group. Levels of serum creatinine showed highly significant increase (P< 0.001) in groups treated with LD50 of crude venom and 5 times LD50 of irradiated venom at 2 and 10 KGy, But, showed significant (P< 0.05) with groups treated with 5 times LD50 of irradiated venom at 5KGy & LD50 of irradiated venom<br />at 10 KGy showed non-significant in group treated with LD50 of 5KGy & 2KGy compared to control group. Histological examination showed alterations in liver, kidney in mice injected with crude venom compared to control group. Also, the group of mice which injected with different doses of irradiated venom showed no histopathological alteration in liver and kidney tissues, except the mice group injected with 5 times LD50 of irradiated venom at 2 KGy and the group which injected with 5 times LD50 of irradiated venom at 10 KGy which showed alterations in liver and kidney tissues.https://jesp.journals.ekb.eg/article_68129_4d4dbd8bdef8567b0bf5744c58b3e0ec.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801COMPARISON BETWEEN DIFFERENT METHODS FOR DIAGNOSIS OF ACANTHAMOEBA INFECTION AND ISOLATION OF GENOTYPE T9 FROM A CONTACT LENS CASES2652766813010.21608/jesp.2019.68130ENMOHAMMED M.ABDEL-GHAFFARDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt.MONA M.EL SOBKYDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt.NANCY M.HARBADepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt.AMANY A.RADYDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt.NOHA A.ABOKHALILDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt.Journal Article20200114Acanthamoeba genus is a medically important free living amoeba causing serious humans infections. Amoebic keratitis (AK) is a sight threatening infection of cornea caused by Acanthamoeba pathogenic genotypes, which prevalence remarkably increased in developed countries. The study compared different methods for diagnosing AK and identified Acanthamoeba genotypes by molecular examination in contact lens wearers (CLWs). Patients were 79 clinical corneal swaps (CS) and 15 samples from contact lens storage cases (CLSC). Clinical CSs were divided into four groups; GI: 20 patients suffering from chronic corneal ulcers, GII: 15 patients with traumatic ulcers, GIII: 24symptomatic CLWs and GIV: 20asymptomatic control individuals. CLSC were provided from apparently healthy asymptomatic CLWs (15). Swabs and solution samples were underwent microscopic and staining examination, cultivation on non-nutrient agar (NNA) plates and<br />PCR molecular analysis. Sequencing and genotyping of PCR- positive samples were performed. The results showed that Acanthamoeba parasites were detected in 3.8% of CS and 6.7% of CLSC samples. The highest significantly positive results were by culture (3.8%) followed by Giemsa and trichrome stains (2.5%) and lastly direct microscopy (1.3%) of CS samples. Only one positive sample (6.7%) was detected in CLSC by all methods, but without statistical significance.<br />Sensitivity of PCR compared to culture was 25%. Acanthamoeba parasites in CS were from subgroup II with 12.5% detection rate in CLWs, but the positive case from CLSC was from subgroup I with 6.7% detection rate. This study confirmed different risk factors in association with AK in CLWs. Genotype determination for Acanthamoeba positive case by PCR revealed homology with Acanthamoeba genotype T9 isolate ICS20.https://jesp.journals.ekb.eg/article_68130_4d7851f01321ff5b20d9be7fb580aba9.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EFFECT OF LEPTIN RECEPTOR POLYMORPHISM ON EGYPTIAN CHILDREN WITH ENTAMOEBA HISTOLYTICA INFECTION2772826813110.21608/jesp.2019.68131ENSOHA I.AWADDepartment of Parasitology, Faculty of Medicine, Mansoura University.RAIDA S.YAHYADepartment of Laboratories, Children Hospital, Faculty of Medicine, Mansoura University.EMANHAMEDDepartment of Laboratories, Children Hospital, Faculty of Medicine, Mansoura University.Journal Article20200114Entamoeba histolytica is a protozoan parasite that causes amebic colitis, an invasive disease responsible for as many as 100,000 deaths per year globally. Leptin signaling protects from amebiasis, with query molecular mechanism. This study aimed to evaluate the effect of leptin Receptor Q223R Polymorphism on Egyptian children with Entamoeba histolytica infection. After ethical clearance, blood and fecal samples were taken from one hundred children with E. histolytica and 100 children as control group. All children were subjected to anthropometric assessment. Entamoeba infected group was further assessed for severity of symptoms, cyst load, and ELISA for differentiation of E. histolytica from E. dispar. Q223R LEPR polymorphism then performed for both groups. RR was higher in children with E. histolytica group while QQ and QR were higher in control group. Heavy cyst count, severe underweight, severe symptoms, and median leptin level was higher in RR group compared to QQ & QR groups. The highest risk of R allele was severe underweight, severe symptoms and high leptin level.https://jesp.journals.ekb.eg/article_68131_ff4745cb91ad706bfd21dbfcf2b0f246.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801ONCOGENIC LIVER FLUKES ACQUIRED FROM EATING RAW OR UNDERCOOKED FISH AND CRABS2832926813210.21608/jesp.2019.68132ENWAEL MORSY A.EL-SAYEDMilitary Medical Academy, Cairo11291, Egypt.ASHRAF A.ABOU-BAKRMilitary Medical Academy, Cairo11291, Egypt.TOSSON A.MORSYFaculty of Medicine, Ain Shams University, Cairo 11566, Egypt.Journal Article20200114Liver flukes as Clonorchis sinensis and the similar genus Opisthorchus felineus and O. viverrini are considered oncogenic as causing cholangiocarcinoma in about ten percent of cases where millions are infected in southeast Asia and Europe being endemic there and mostly asymptomatic but require treatment. Klatskin tumour is bile duct bifucational area carcinoma affection that is almost unique for these infections which comes by eating raw or undercooked fish and crabs. There has been strong evidence that both types of liver flukes are present in Egypt and Middle East being endemic since the snail first intermediate hosts is so many fresh water snails and risks are also, still similarly, to millions of humans, who require treatment to halt progression of disease in asymptomatic cases. This review aimed to clarify those types of flukes for health care workers and the public for better diagnosis, prevention and control, Also, to describe the main traits of C. sinensis and O. viverrini, their molecular biology and core carcinogenic mechanisms in a contrast pattern.https://jesp.journals.ekb.eg/article_68132_748c9e31e083eedfbefba92bf73d9f8b.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801IMMUNOMODULATORY EFFECTS OF PAEONIFLORIN COMBINED TO SOLUBLE EGG ANTIGENS IN SCHISTOSOMIASIS MANSONI MURINE MODEL2933006813310.21608/jesp.2019.68133ENNAGLAA FATHYABD EL-AALDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.EMAN HASSANABD ELBARYDepartment of Pathology, Faculty of Medicine, Zagazig University, Egypt.Journal Article20200114Praziquantel, the main schistosomiasis chemotherapy; cannot fully counteract the infection associated morbidity including hepatic fibrosis and its sequelae. A novel therapeutic strategy is mandatory. The current study is a novel trial to assess the immunomodulatory effect of Paeoniflorin (PAE) in combination to soluble egg antigen (SEA) in schistosomiasis mansoni murine model through; parasitological, histopathological, immunohistochemical and serological studies aiming to synergistic effect that not only eliminate the parasite but also ameliorate morbidity associated to infection. Forty laboratory bred Swiss albino male CD-1 mice were used. The mice were classified into five groups (8 mice each), control healthy, control infected, PAE treated (50 mg/kg/d), SEA vaccinated (50μ protein) and combined (PAE+SEA) groups. All mice groups were sacrified 10 weeks post infection. Our results showed marked decrease in egg count/gm stool, worm burden, granuloma diameter, tissue transforming growth factor-beta1 (TGF-β ) serum interleukin-13 (IL-13) associated with marked increase in the serum interferon-gamma (INF-γ) level in (PAE+SEA) combined group compared to all groups.https://jesp.journals.ekb.eg/article_68133_24a51d8d030c59669d815fb5dacf0dec.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801THERAPEUTIC EFFICACY OF CHITOSAN NANOPARTICLES LOADED WITH ALBENDAZOLE ON PARENTERAL PHASE OF EXPERIMENTAL TRICHINELLOSIS3013116813410.21608/jesp.2019.68134ENNASHAAT E.NASSEFDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.ISMAIL M.MOHARMDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.AMANY F.ATIADepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.REHAM M.BRAKATDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.NOHA M.ABOU HUSSIENDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.ASMAASHAMSELDEENDepartment of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin el-Kom, Menoufia, Egypt.Journal Article20200114Most of the drugs used for trichinellosis treatment showed a limited bioavailability, a high degree of resistance and a weak activity against encapsulated larvae. Therefore, there is an urgent need to develop new agents to improve the bioavailability of these drugs. So, the aim of the present study was to assess the use of chitosan (CH) nanoparticles alone or loaded with full and half dose albendazole (ABZ) to increase albendazole dissolution rate, to enhance its antiparasitic activity during the muscular phases of T. spiralis infection. Fifty male albino mice were used. They were divided into six experimental groups. Two control groups each includes five mice and four infected treated groups each includes ten mice. Chitosan nanoparticles were used orally at a dose of 100mg/kg/day starting from the 31st -day post infection (dpi) for seven successive days either alone or loaded with full dose or half dose of ABZ. Results revealed a significant improvement in all treated groups with the highest reduction rate (97.3%) of muscle larval counts, improvement of muscular histopathological changes, and degeneration of encysted larvae with minimal pathologic changes of infected skeletal muscles. A significant decrease in inducible nitric oxide synthetize (iNOS) expression in muscle tissues was in mice treated by CH loaded with a full dose of ABZ compared to control group.https://jesp.journals.ekb.eg/article_68134_933257de6e5058cf6f45fd6589f42ba0.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801MALARIA AND HUMAN IMMUNODEFICIENCY VIRUS (HIV) IN REPUBLIC OF SOUTH SUDAN: A MINI-OVERVIEW3133206813510.21608/jesp.2019.68135ENAHMAD MEGAHED A.SALEHMilitary Medical Academy, Cairo11291, Egypt.MOSTAFA M.ELNAKIBMilitary Medical Academy, Cairo11291, Egypt.DENG MAYOM AROPMALEKSouth Sudan People’s Defense Forces
(SSPDF), Egypt.TOSSON A.MORSYFaculty of Medicine, Ain Shams University, Cairo 11566, Egypt.Journal Article20200114South Sudan is the 42nd-most densely populated African country (19.2 inhabitants / km²). The top 10 African countries by population density are: Mayotte (France), Mauritius, Rwanda, Burundi, Comoros, Réunion (France), Seychelles, Nigeria, São Tomé and Príncipe, Gambia, Uganda, Malawi. South Sudan has the 36th-largest legislature in the world by number of members (382 totals). The top 10 legislatures by number of members are: China, United Kingdom, Italy, France, India, European Union, Germany, Japan, Indonesia, Korea, and North. South Sudan is the 76th-largest country in the world by population (12,230,730, based on estimates published by the United Nations). The top 10 countries by population (United Nations) are: World, China, India, United States, Indonesia, Brazil, Pakistan, Nigeria, Bangladesh, Russia, and Mexico. This is a mini-view to raise awareness about malaria and HIV.https://jesp.journals.ekb.eg/article_68135_4a5c83c425efd9459613d54b25cd022f.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EFFECT OF ARTEMISIA SANTONICA ON SCHISTOSOMA MANSONI INFECTED MICE: PARASITOLOGIOCAL, HISTOPATHOLOGICAL AND SCANNING ELECTRON MICROSCOPICAL STUDIES3213326813710.21608/jesp.2019.68137ENHEND M.TAGDepartment of Biology, Faculty of Sciences and Arts-Khulais, Jeddah University,
Jeddah P.O. Box 355, Code 21921, Saudi Arabia; Department of Zoology, Faculty of
Sciences, Suez Canal University, Ismailia,MONA M.HENEDAKDepartment of Zoology, Faculty of Science,
El Arish University, Egypt.NAHLA H.SALLAMDepartment of Veterinary Parasitology, Suez Canal University, Ismailia, Egypt.EHSSAN A.HASSANDepartment of Biology, College of Science and Humanities
in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942 Saudi Arabia.RAWIA A.ZAYEDDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Egypt.SAMAH TALAATMOHAMMEDDepartment of Zoology, Faculty of Science,
El Arish University, Egypt.Journal Article20200114Schistosomiasis is one of the most wide spread tropical diseases. Millions of people suffer severe illness related to schistosomiasis. This study evaluated the anti-parasitic effects of crude Artemisia santonica and their aqueous and hexane fractions against Schistosoma mansoni infected mice by some parasitologiocal and histopathological studies as worm load, liver egg load, intestine egg load, oogram pattern and also by surface ultrastructure of recovered worms using SEM. The result reflected that mice treated with crude extracts of A. santonica and their aqueous fractions gave a promise anti-inflammatory and anti-schistosomal agent.https://jesp.journals.ekb.eg/article_68137_87f650ba33f899c672693252f75064e8.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EFFECT OF KILLING METHODS AND PRESERVATIVE SOLUTIONS ON THE LARVAL BODY LENGTH OF CHRYSOMYA MARGINALIS (DIPTERA: CALLIPHORIDAE)3333366813810.21608/jesp.2019.68138ENFATMA K.ADHAMDepartment of Entomology, Faculty of Science, Cairo University, Egypt.SALWA S.RASHEDDepartment of Zoology, Entomology Section, Faculty of Science, Zagazig University, Zagazig, Egypt.ZEINAB M.EL-BASHEIRDepartment of Zoology, Entomology Section, Faculty of Science, Zagazig University, Zagazig, Egypt.EMAN E.ZAHERDepartment of Zoology, Entomology Section, Faculty of Science, Zagazig University, Zagazig, Egypt.Journal Article20200114Estimation of the minimum post mortem interval (PMI) is the essential role of forensic entomology. The accurate determination of this interval depends on the correct larval identification and accurate estimation of larval age. One method of larval age estimation is the larval length measurement. Killing methods and the type of preservative solutions can greatly affect the larval length and therefore reduce the accuracy of PMI estimation. This study was conducted to determine which killing method and preservative can preserve the best larval length of Chrysomya marginalis. Third instar C. marginalis larvae were either placed live in the preservatives, 10% formalin, 70% ethanol, XAA, Kahleˊs solution and Pampleˊs fluid, or killed by boiling water (BWK) before being placed in the same preservatives. The larval length was recorded after day 1, 3, 5, 7 and 15 and for BWK larvae only; the length was recorded immediately after killing the larvae and before being placed in the preservatives. The larval length of C. marginalis was significantly affected by killing method (live or BWK) and the type of the preservative used. The mean length of the larvae placed live in the preservatives<br />decreases significantly than those killed by boiling water before being placed in the preservatives.It was observed that killing larvae by boiling water then preserved in 10% formalin and Kahleˊs solution is the best method for preserving the larval length, but 10% formalin is not recommended if DNA recovery is desired.https://jesp.journals.ekb.eg/article_68138_d6287663be2f84782490e21b0ca131bd.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801UNDERWEIGHT AS A RISK FACTOR FOR MULTIPARASITISM AND ITS CLINICAL SIGNIFICANCE AMONG EGYPTIAN CHILDREN3373446813910.21608/jesp.2019.68139ENMONA M.KHATERDepartment of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.EMAN I.ELFARDepartment of Public Health and
Community Medicine, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.Journal Article20200114Although multiparasitism is a common phenomenon, but its impact on young children nutritional and developmental status is underestimated. This cross-sectional study aimed to statistically correlate single/multiple parasitic infections detected with underweight, demographic and clinical data. Stool samples were collected from 222 Egyptian children from 1 to 5 years old who were attending the Diagnostic and Research Unit of Parasitic Diseases. Stool samples were screened for parasitic species using light microscopy of direct, trichrome and modified kinyoun stained smears. Nutritional status was evaluated using weight-for-age plotting against standard reference growth charts. Questionnaire was used to collect socio-demographic and clinical data of children under study. The overall prevalence of parasitosis was 51.8%. Among infected children, polyparasitism was significantly higher among underweight (66.7%; P<0.001), and in males compared to females (31.6% vs. 16.7%; P =0.011). Univariate analysis showed significant associations between underweight<br />and Giardia lamblia co-infection with other species (P < 0.001). Multiparasitism should be of more concern especially to this age group, as it can exacerbate children’s morbidity. However, several methodological challenges remain when it comes to detecting parasite interactions in resource-constrained settings.https://jesp.journals.ekb.eg/article_68139_26b1a9852da399cac615ba08bef78e54.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801HISTOPATHOLOGICAL AND ULTRASTRUCTURE FEATURES OF HYDATID CYST FROM SLAUGHTERED ANIMALS IN EGYPT3453566814010.21608/jesp.2019.68140ENABD ALLAH A. A.AL HOOTDepartment of Zoology, Faculty of Science, Zagazig University.HESHAM M.SHARAFDepartment of Zoology, Faculty of Science, Zagazig University.AMIRA I.EL-KABBANYDepartment of Zoology, Faculty of Science, Zagazig University.SHIMAA S.HASSANDepartment of Zoology, Faculty of Science, Zagazig University.Journal Article20200114In the current study, the hydatid cyst-induced tissue lesions in liver and lung of camels and sheep infected with Egyptian strain of E. granulosus was evaluated and the fine structures of the protoscolices by scanning electron microscope (SEM) was described. The results indicated that both fertile and sterile hydatid cysts could lead to various degree of tissue lesions visualized mainly as necrosis, inflammatory cell infiltration and tissue degeneration other histopathological changes occurred in lung and liver of infected animals due to infection with hydatid cyst. Tissue lesion associated with hydatidosis was described. SEM showed envaginated, invaginated protoscolex and the broad capsule of hydatid cyst. There were two types of hooks, large (upper row) and small (lower row) in hydatid cyst protoscolices; with microtriches on different regions.https://jesp.journals.ekb.eg/article_68140_c5a791e596119c0ff065873dba3dba82.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801CYTOCHROME OXIDASE SUBUNIT AND INTERNAL TRANSCRIBED SPACER MOLECULAR REVELATION OF INFECTION WITH FASCIOLA SP. IN FIELD- COLLECTED LYMNAEA NATALENSIS SNAILS3573646814110.21608/jesp.2019.68141ENAMINA M.IBRAHIMDepartments of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.SAMI M.NASRDepartments of Biochemistry,
Molecular Biology and Medicinal Chemistry, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.Journal Article20200114Fasciolosis is one of the neglected tropical diseases that negatively affect the farming industry and public health. The present study aimed to evaluate a polymerase chain reaction (PCR) assay for detection of infection with Fasciola sp. in field-collected Lymnaea natalensis snails from two rural localities in Egypt; and to compare between it and the ordinary microscopic methods. A total of 1000 lymnaeid snails were collected, and then the samples were examined for the infection by crushing, cercarial shedding and molecular methods. The average sensitivity of microscopic methods compared to PCR was 56.15% and this gives the indication that the molecular techniques were highly sensitive, specific and reliable methods for detecting Fasciola sp. The study detected two genes; nuclear ribosomal central internal transcribed spacer 1 (ITS1856bp) and mitochondrial cytochrome oxidase subunit I (CO1 831bp). Gel electrophoresis<br />showed strong bands for these single snails' samples and confirmed the presence of infection in both localities. Molecular techniques gave sensitive, accurate identification of infection, established prevalence in endemic areas and planning time for proper control strategy.https://jesp.journals.ekb.eg/article_68141_a9ff985a883aee735d96b72be661e2ce.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801HISTOLOGIC PARAMETERS FOR STUDYING MULTIPLE BLOOD MEALS IN AEDES ALBOPICTUS (SKUSE) (DIPTERA: CULICIDAE)3653726814210.21608/jesp.2019.68142ENABEER S.YAMANYDepartment of Zoology, Faculty of Science, Zagazig University, Egypt.FATMA K.ADHAMEntomology of Department, Faculty of Science, Cairo University, Giza, Egypt.HEINZMEHLHORNInstitute of Zoology, Cell Biology and Parasitology, Heinrich-Heine University,
Duesseldorf, Germany.Journal Article20200114During a single gonotrophic cycle, histologic technique was used to detect the multiple blood feedings in Ae. albopictus. During a 3-day period, 200 blood engorged Ae. albopictus mosquito females were examined. The results showed that 69% (138/200) of these females imbibed two blood meals. Time between the second blood meal and fixation ranged from 0 to 32 h. When applying histologic parameters, only 23% of females with multiple meals were detected. Our data suggest that the limit for histologic detection of multiple feeding were more restricted for Ae. albopictus. Histological studies revealed the presence of an early blood meal within the second one appearing as a body of dark digested blood mixed with heme (H), or sometimes a pink undigested blood associated with a bluestaining peritrophic plug (PP) surrounding the blood meal and the presence of a physical separation (PS) separating the blood meals. When using histologic technique of field collected mosquitoes, advanced stages of oocytes or development of ovarian follicles with<br />apparent dilations were observed.https://jesp.journals.ekb.eg/article_68142_e9484a7a6fec7b314fde3a9389b24ddf.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801ELECTRON MICROSCOPY OF BLASTOCYSTIS HOMINIS AND OTHER DIAGNOSTIC APPROACHES3733806814510.21608/jesp.2019.68145ENMONA HASSANELSAYADDepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.MONA MOHAMEDTOLBADepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.HANIA AHMEDARGIAHDepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.AHMEDGABALLAHDepartment of Microbiology, Medical Research Institute, Alexandria University, Egypt.MERVAT MOSTAFAOSMANDepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.IRENE LEWISMIKHAELDepartment of Hematology, Medical Research Institute, Alexandria University, Egypt.Journal Article20200114This work evaluated light microscopy and culture against conventional PCR in diagnosing Blastocystis hominis in human stool samples, and were studied by scanning and transmission electron microscope. Hundred stool samples were acquired from the Parasitology laboratory of the Medical Research Institute in Alexandria. All samples were subjected to light microscopy, culture and conventional PCR to diagnose B. hominis in human stool samples; subsequently B. hominis obtained from culture media were further studied by scanning and transmission electron microscope.<br />The results showed B. hominis was detected in 52/100 studied stool samples using light microscope, xenic culture showed 65 positive samples, 67 samples were positive by conventional PCR. SEM revealed the outer surface of B. hominis and rosette-shaped dividing oocysts while TEM demonstrated internal structures of the organisms.https://jesp.journals.ekb.eg/article_68145_a1b383aa89f8678be78b3c366b403c24.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EFFICACY OF LEAVES AQUEOUS EXTRACT AND SYNTHESIZED SILVER NANOPARTICLES USING LAGENARIA SICERARIA AGAINST CULEX PIPIENS LISTON AND ANOPHELES PHAROENSIS THEOBALD3813876814810.21608/jesp.2019.68148ENAHMED Z. I.SHEHATADepartment of Zoology, Faculty of Science, Al-Azhar
University, Cairo 11651, Egypt.AMMAR M.MAHMOUDDepartment of Chemistry, Faculty of Science, Al-Azhar
University, Cairo 11651, Egypt.Journal Article20200114The control of Culex pipiens and Anopheles pharoensis considered as important strategy in eliminating diseases transmitted by the two mosquito species. Application of plantderived products in mosquitos' control reported as much effective, less expensive and safer to human, as well as environment than their synthetic counterparts. The present study investigated the activity of Lagenaria siceraria leaves aqueous extract and L. siceraria- synthesized silver nanoparticles (AgNPs) against immature stages of C. pipiens and A. pharoensis. Immature stages of both tested mosquito species were exposed to 50, 100, 200, 300 and 400 ppm of L. siceraria leaves aqueous extract and 5, 10, 20, 30 and 40 ppm of L. sicerariasynthesized AgNPs for 24 h. It was found that L. siceraria leaves aqueous extract can reduce aqueous silver ions to generate silver nanoparticles suspended in water. The results of DLS revealed that, the average hydrodynamic diameter calculated for AgNPs was 33.25 nm, while the TEM images showed the occurrence of individual silver nanoparticles<br />(13.38-27.70 nm) and a number of aggregates in the test suspension. In addition, L. siceraria- synthesized AgNPs were more toxic against tested mosquito species than L. siceraria leaves aqueous extract. The LC50 values recorded 15.2, 18.2, 22.5, 24.7 and 29.4 ppm against C. pipiens first to fourth instar larvae and pupae; whereas LC50 values were 11.9,<br />14.5, 17.7, 19.8 & 23.1ppm against A. pharoensis first to fourth instar larvae and pupae, respectively.https://jesp.journals.ekb.eg/article_68148_a2b279b2a81c5b3e86ee1153f058883a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EFFICACY OF NIGELLA SATIVA (HABBET EL-BARAKA) AS A TREATMENT FOR CHILDREN AND ADOLESCENTS INFECTED WITH ENTAMOEBA HISTOLYTICA COMPLEX: A RANOMIZED CONTROLLED CLINICAL TRIAL3894006815410.21608/jesp.2019.68154ENSAMAR F.MOHAMEDDepartment of Family Medicine, Faculty of Medicine, Suez Canal University, Ismailia, 45122, Egypt.SHAHIRA A.AHMEDDepartment of Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, 45122, Egypt.MOSLEH ABDEL RAHMANISMAILDepartment of Family Medicine, Faculty of Medicine, Suez Canal University, Ismailia, 45122, Egypt.SAFWAT A.AHMEDDepartment of
Pharmacognosy, Faculty of Medicine, Suez Canal University, Egypt.MOHAMED M.EIDADepartment of Endemic Medicine & Infectious Diseases,
Faculty of Medicine, Suez Canal University, Egypt.KHALID SAIDHEISSAMDepartment of Family Medicine, Faculty of Medicine, Suez Canal University, Ismailia, 45122, Egypt.Journal Article20200114Amoebiasis is caused by Entamoeba histolytica infection. In African countries amoebiasis is endemic and one of the top 10 causative agents of diarrhea in children. The common diagnostic method for Entamoeba spp. in Egypt is microscopic observation and identification of cysts/trophozoites in fecal samples. It is known that metronidazole (MTZ) is the choice of treatment for amoebiasis. However, drug resistance and side effects are considered to be disadvantages. In this study children and adolescents infected with E. histolytica complex (Eco) have been divided into two groups. As a control treatment, one group received MTZ capsules. The other group (intervention) received herbal medicinal capsules of Nigella sativa (N. sativa) powder to test for its effectiveness against Eco infection and compare its results with the control group. Socio-demographic data, cure rate, recession of symptoms and residual side effects of treatment were evaluated for the patients. In the intervention group, N. sativa showed a cure rate of 78.8% compared to 91.2% in MTZ- control group with no significant difference. Eco infected patients complained about diarrhea, bloody stool and abdominal pain with significant reduction in N. sativa- control group symptoms (from 93.9 to 15.2 %) compared to MTZ. Treatment with MTZ caused several symptomatic residual side effects of metallic taste, anorexia, abdominal pain and diarrhea. After N. sativa treatment, however, no residual symptoms were noted, an indicator of its safety. N. sativa was safe, cost-effective, herb with potential promising effectiveness against Eco infection.https://jesp.journals.ekb.eg/article_68154_1793bc1a149ab87b8cf34087303d6836.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801SEROLOGY OF THE SYSTEMIC LUPUS ERYTHEMATOSUS IN EGYPTIAN PATIENTS4014046815910.21608/jesp.2019.68159ENALYAA A.FARIDFaculty of Science, Cairo University, Giza, Egypt.RWAN A.EISSAFaculty of Biotechnology, October
University for Modern Sciences and Arts (MSA), 6th October, Egypt.AHMED M.NADAFaculty of Biotechnology, October
University for Modern Sciences and Arts (MSA), 6th October, Egypt.MONA M.ELAMIRFaculty of Biotechnology, October
University for Modern Sciences and Arts (MSA), 6th October, Egypt.AZZA M.ELAMIRFaculty of Science, Cairo University, Giza, Egypt.Journal Article20200114Systemic lupus erythematosus (SLE) is a prototypic multisystem autoimmune disease with a vast spectrum of clinical presentations including almost all body organs and tissues. The incidence of lupus has tripled in the last 40 years, mainly due to the improved diagnosis. For more than 60 years the presence of autoantibodies in patients with SLE has been known. A great effort is being made for understanding the diagnostic, pathogenic and prognostic meaning of these autoantibodies. The study demonstrated the significance of autoantibodies test in Egyptian SLE patients. The results showed that 93.3% of patients have positive ANA test, 90% have positive anti-dsDNA. Anti-Sm antibodies were in 40% and 49.3% of patients have positive anti-nucleosome antibody test. Also, 30.6% of patients deve-loped a positive anti-histone test & 26% have positive anti-ribosomal test. Anti-phospholipids antibodies were found in 44.6% of patients. Levels of C3 & C4 (0.552±0.43 & 0.125±0.12, respectively) were lower than that of control group (1.2±0.35 & 0.42±0.54 for C3 & C4, respectively).https://jesp.journals.ekb.eg/article_68159_331f48b2cb3a59a0401e89e5a481a8c6.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801RELATIONSHIP BETWEEN ASCARIS LUMBRICOIDES INFECTION AND BRONCHIAL ASTHMA IN JUHAYNA CITY, SOHAG, UPPER EGYPT4054086816310.21608/jesp.2019.68163ENAMAL M.AHMEDDepartments of Medical Parasitology, Faculties of Medicine, Sohag University, Egypt.NASR EL DEEN M. M.ALIAl-Azhar University, Assiut Branch, Egypt.Journal Article20200114Bronchial asthma is a chronic inflammatory disorder of the airways characterized by airway hyper responsiveness and reversible airflow obstruction that fluctuates over time. Parasitic infection is one of the environmental factors associated with a small increase in bronchial asthmatic risk. In the present study, 200 stool samples were collected from patients suffering from bronchial asthma and 200 stool samples from non-asthmatic healthy volunteers as control group. The samples were preserved in 10% formalin and examined macroscopically, microscopically by direct smear & formol ether sedimentation techniques. It was found that 12/200 cases (6%) were infected with Ascaris lumbricoides in asthmatic group and 2/200 cases (1%) in control (P = 0.007) illustrating significant difference between cases and control group indicating a role of Ascaris lumbricoides as a risk factor for bronchial asthma.https://jesp.journals.ekb.eg/article_68163_1c76b29a0d8896811c26de99035e613d.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801COMPARATIVE EVALUATION OF MULTIPLE STAINING TECHNIQUES FOR IDENTIFICATION OF DIFFERENT DEVELOPMENTAL STAGES OF ACANTHAMOEBA AND NAEGLERIA4094226818110.21608/jesp.2019.68181ENHANAN EMELDEEKDepartment of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.RASHA AHATTIADepartment of Medical Parasitology, Faculty of Medicine,
Assiut University, Assiut, Egypt.MARTINA MNAGEEBDepartment of Medical Parasitology, Faculty of Medicine,
Assiut University, Assiut, Egypt.ATEF ASAKLADepartment of Medical Parasitology, Faculty of Medicine,
Assiut University, Assiut, Egypt.Journal Article20200114Potentially pathogenic free-living amoebae (FLA) are unique protozoa that present a healthcare challenge. The appropriate choice of staining technique is crucial to improving the efficiency of microscopic diagnosis of FLA isolated from environmental as well as clinical samples. This study aimed to evaluate different staining techniques for morphological<br />identification of Acanthamoeba and Naegleria isolated from tap water samples in different localities in Assiut City, Egypt. A multi-attribute evaluation was performed to compare these techniques considering staining quality, ease of performance, cost and time of each procedure. Seven staining techniques were used including Lugol’s iodine and lactophenol cotton blue as temporary stains, with Giemsa, trichrome, iron hematoxylin, acid-fast and Gram stains as permanent stains. Both lactophenol cotton blue and Lugol's iodine showed good structural details of different stages of FLA and good contrast at a very short time and reasonable cost, followed by Giemsa. Both trichrome and iron hematoxylin stains gave comparable staining quality but at a longer time with more complex staining procedures and higher cost. Both modified acid-fast and Gram stains didn't provide sufficient staining quality to identify morphological details of FLA. According to the multi-attribute evaluation, both Lugol’s iodine and lactophenol cotton blue achieved the highest rank (92.85%) followed by Giemsa and Gram stains (64.28 %), modified acid-fast stain (57.14%), while iron hematoxylin and Masson’s<br />trichrome had the last rank (46.42%).https://jesp.journals.ekb.eg/article_68181_0fa78a5d519dc079fcc02107f8335098.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801OPPORTUNISTIC PARASITIC PULMONARY INFECTIONS IN HUMAN IMMUNODEFICIENCY VIRUS (HIV) INFECTED PATIENTS: WITH REFERENCES TO EGYPTIAN PARASITES4234386818510.21608/jesp.2019.68185ENASHRAF A.ABOU-BAKRMilitary Medical Academy, Cairo11291, Egypt.NASHWA ALSAYEDIBRAHIMMilitary Medical Academy, Cairo11291, Egypt.TOSSON A.MORSYFaculty of Medicine, Ain Shams
University, Cairo 115662, Egypt.Journal Article20200114Prior to the era of potent antiretroviral therapy, parasitic pulmonary infections were more commonly seen than they are today. However, the clinician needs to be aware of the presenting symptoms and signs of these uncommon infections, which may still occur in the immuno-suppressed patient with untreated or drug-resistant HIV infection. The commonest pulmonary parasites are protozoa (toxoplasmosis, amebiasis, cryptosporidiosis and microsporidiosis) and helminthes (paragonimiasis, hydatidosis ancylostomiasis, stronyloidiasis and toxocariasis). It is important to consider parasitic infections in the differential diagnosis of such lung diseases. If parasites are identified early, most parasitosis affect lung is curable with medical or surgical treatments.https://jesp.journals.ekb.eg/article_68185_44a6914ef12bb82f38ac9aadbd1bbe47.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801PREVALENCE AND HISTOPATHOLOGY OF TRICHINELLA SPIRALIS LARVAE OF SLAUGHTERED PIGS IN CAIRO GOVERNORATE, EGYPT4394426818710.21608/jesp.2019.68187ENAHMED K.DYABDepartment of Parasitology, Faculty of Medicine Assiut University, Egypt.MARWA A.AHMEDDepartment
of Pathology, Faculty of Veterinary Medicine,
Aswan University, Egypt.AHMED G.ABDELAZEEMDepartment of Parasitology, Faculty of Veterinary Medicine,
Aswan University, Egypt.Journal Article20200114The present work was planned to study the prevalence and the pathological lesions of Trichinella spiralis larva of slaughtered pigs in Cairo Governorate by using Trichinoscopic examination in slaughterhouse and confirmatory diagnosis by histopathological examination. For this purpose 184 muscle samples of pigs were collected from Al-Basatin Slaughterhouse, Cairo Governorate. The overall prevalence of Trichinella spiralis infection was 1.08% of examined pig's carcasses.https://jesp.journals.ekb.eg/article_68187_127460ef6447093ce43269db81c4254a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801CROSS NEUTRALIZATION OF SOME KINDS OF SCORPION VENOMS FROM AFRICA AND SOUTH EAST USING VACSERA POLYVALENT SCORPION ANTISERA4434496818910.21608/jesp.2019.68189ENABIR A.ELFIKYVACSERA, Dokki, Giza, Egypt.HODA R.ATTIAFaculty of Biotechnology, October University for
Modern Science and Arts, October City, Giza, Egypt.MOAAZ R.ABD ELAZIZFaculty of Biotechnology, October University for
Modern Science and Arts, October City, Giza, Egypt.Journal Article20200114Preparation of scorpion antivenin includes administration of the venom to a suitable animal - mostly horses- and after an appropriate period collecting the specific antibodies from the serum of the inoculated animal. During such procedure the recipient animal may suffer different types of ill-health signs including, generalized asthenia, pallor, skin rashes, muscular pain, hemorrhages, cardiovascular, respiratory problems, nervous signs as paresis and paralysis, break down of tissues, and finally collapse and death, The severity and duration of the observed clinical signs depend on the nature, amount and site of the injected venoms Genus scorpion is widespread throughout Western and Central Asia. It is a genus in constant revision and recognizes a number of subspecies. An extensive study was conducted of neutralization of lethality of four species of scorpions from Egypt including Leurus quinquestriatus, Androctonus crassicauda, Androctonus amorexi, and Androctonus australis and three species of scorpions from Yemen including Leurus quinquestriatus, Bothus spp. and Androctonus australis by VACSERA equine scorpion antisera. The results revealed that LD50 venoms was 6.9,<br />8.3, 12.5, & 6.94 LD50/mouse consequently for Egyptian venoms, while LD50 of the previous mentioned venoms from Yamen was 6.4±6, 20.7 & 7.0 LD50/mouse consequently. Polyvalent scorpion venom antisera by injection of horses by L. quinquestriatus, A. crassicauda proved effective in neutralizing specifically Egyptian L. quinquistriatus, and A. crassicauda, by 70, & 35 LD50/mouse, and paraspecifically other scorpion species including Egyptian A. amorexi, and A. australis by 45, and 60 LD50/mouse. Polyvalent scorpion venom antisera neutralized Yameni species as follow 70 LD50/mouse for Leurus, 60 LD50/mouse for A. australis, and 20 LD50/mouse for Bothus spp.https://jesp.journals.ekb.eg/article_68189_0701e1c9d2a3f5efb34526d9ad483fc4.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801ANTI-NUCLEAR ANTIBODY (ANA) PATTERNS IN EGYPTIAN SYSTEMIC LUPUS ERYTHEMATOSUS4514546819010.21608/jesp.2019.68190ENAZZA M.ELAMIRDepartment of Zoology, Faculty of Science, Cairo University, Giza, Egypt.ALYAA A.FARIDDepartment of Zoology, Faculty of Science, Cairo University, Giza, Egypt.ESRAA S.AMINFaculty of Biotechnology, October University for Modern Sciences and Arts (MSA),
6th October City, Giza, Egypt.HEBA A.HASSANFaculty of Biotechnology, October University for Modern Sciences and Arts (MSA),
6th October City, Giza, Egypt.MOHAMED M.MAGEDFaculty of Biotechnology, October University for Modern Sciences and Arts (MSA),
6th October City, Giza, Egypt.AHMED A.AREFFaculty of Biotechnology, October University for Modern Sciences and Arts (MSA),
6th October City, Giza, Egypt.Journal Article20200114In fact autoantibodies are immunoglobulins against self-antigens that are known as endogen antigens. Systemic lupus erythematosus (SLE) is an occasion that the body begins a fight against its own cells and organs. There are different types of autoantibodies that have been identified in recent years. The most familiar is anti-nuclear antibodies (ANA). They are created against cell nuclei, and one of the methods used for its detection and its pattern determination is indirect immunofluorescence test (IIF). IIF was used for that purpose in our study, and concluded that ANA positivity rate was higher in female patients than the male ones, where ANA was positive in 36.1% of the male patients and 48.2% of female patients. Also, the most frequent ANA patterns in SLE patients were speckled pattern, where 21.6% & 41.3% of male<br />and female patients, respectively, have speckled pattern of ANA.https://jesp.journals.ekb.eg/article_68190_25d60bf77ddcad1643421146d312eef0.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801LARVICIDAL EFFECT OF ALKALOIDS EXTRACTED FROM BITTER LUPIN SEEDS AGAINST MOSQUITOES (CULEX PIPIENS), FLIES (MUSCA DOMESTICA) AND FLEAS (XENOPSYLLA CHEOPIS) UNDER LABORATORY CONDITIONS IN EGYPT4554646819210.21608/jesp.2019.68192ENMARWA E.HASSANDepartment of Toxicology, Research Institute of Medical Entomology, Dokki, Giza, Egypt.NOUR EL-DIN S.ALYDepartment of Toxicology, Research Institute of Medical Entomology, Dokki, Giza, Egypt.MICHEAL W.MIKHAILDepartment of Toxicology, Research Institute of Medical Entomology, Dokki, Giza, Egypt.Journal Article20200114The present work investigated the larvicidal efficacy of alkaloids extracted from Lupin seeds by water (aqueous extract) and methanol (methanol extract) against mosquitoes larvae (Culex pipiens), flies larvae (Musca domestica) and fleas larvae (Xenopsylla cheopis) under laboratorycondition in Egypt. Alkaloids were analyzed by gas chromatography – mass spectrometry (GCMS). Analysis revealed the presence of lupanine (100%), 13-OH-Lupanine (16.48%) and13α- Angeloyloxylupanine (1.3%) in methanol extract of Lupinus luteus and α- isolupanine (100%) only in aqueous extract of lupinus luteus. The lethal concentration LC50 and LC90 were obtained from the established regression log concentrate-response lines after 24 hours. Data indicated that the efficacy of methanol extract of alkaloids against Culex pipiens larvae was more effective than the effect of aqueous extract of alkaloids. The values of lethal concentration of methanol extract of alkaloids were 0.79 and 1.17 mg/ml for LC50 and LC90 respectively, while the values of lethal concentration of aqueous extract of alkaloids were 5.43 and 8.50 mg/ml for LC50 & LC90 respectively. Data also indicated that the efficacy of methanol extract of alkaloids against Musca domestica larvae was effective than the effect of aqueous extract of alkaloids. The values of lethal concentration of methanol extract of alkaloids were 6.40 and 19.18 mg/ml for LC50 &LC90 respectively, while the same values of aqueous extract of alkaloids were 11.20 and 34.00 mg/ml for LC50 and LC90 respectively. In addition, the efficacy of methanol extract of alkaloids against Xenopsylla cheopis larvae was more effective than the effect of aqueous extract of alkaloids. The lethal values concentration of alkaloids methanol extract were 10.56, 19.20 and mg/ml for LC50 &LC90 respectively, but alkaloids values of aqueous extract were 23.21 and 41.69 mg/ml for LC50 & LC90 respectively.https://jesp.journals.ekb.eg/article_68192_25211227c640e61b2b426c9792fe7c0a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801MILÁČEK-VITOVEC STAINING METHOD VERSUS CONVENTIONAL AND MOLECULAR ONES FOR DIAGNOSIS OF CYCLOSPORA CAYETANENSIS INFECTION4654726819610.21608/jesp.2019.68196ENEMAN M.HUSSEINDepartment of Medical Parasitology, Faculty of Medicine, Suez Canal University,
41522, Ismailia, Egypt.AMAL M.ALMATARYDepartment of Medical Parasitology, Faculty of Medicine, Assiut University, 71515, Assuit, EgyptJournal Article20200114Stool samples from 150 immunocompromised patients (including 120 Gastrointestinal [GI] symptomatic and 30 GI asymptomatic) suspected to have Cyclospora cayetanensis infection were examined to evaluate the validity of Miláček-Vitovec staining method in comparison with kinyoun stain for the diagnosis of cyclosporiasis. All results were confirmed with nested PCR as a gold standard. Validation parameters included sensitivity, specificity, accuracy index, positive predictive value and negative predictive value. In addition, infection intensity was compared among all positive samples. DNAs of Cyclospora oocysts were detected among 30/120 (25%) of GI symptomatic patients versus 1.7 % of GI asymptomatic (5/30). Miláček-Vitovec stain showed more validity than kinyoun with a sensitivity reached 80% versus 73.4% (28 versus 25 from 35 cases) while the specificities of showed 90.4% (104/115) versus 81.7% (94/115), respectively. Mild infection intensity was detected among 10 patients only three of them (30%) were detected by Miláček-Vitovec stain while only one (10%) was detected by Kinyoun, Moderate infection were identified among 10 patients all of them were diagnosed by Miláček-Vitovec stain and 9 (90%) was detected by Kinyoun stain. Heavy infection was detected among 15 patients all diagnosed with Miláček-Vitovec stain while 14 were detected with kinyoun.https://jesp.journals.ekb.eg/article_68196_1f57ad47198361625673ea4e535da6c7.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349220190801EVALUATION OF LAPAROSCOPIC ULTRASOUND AS A ROUTINE METHOD FOR ASSESSMENT OF COMMON BILE DUCT PATENCY IN PATIENT WITH HISTORY OF CALCULAR OBSTRUCTIVE JAUNDICE4734786819910.21608/jesp.2019.68199ENAHMED F.ELKASEDDepartment of General Surgery, Faculty of Medicine, Menoufia University, Shebin-
El-Kom, Menoufia, Egypt.AWATEF E.FARGALYDepartment of General Surgery, Faculty of Medicine, Menoufia University, Shebin-
El-Kom, Menoufia, Egypt.YOUSSEF F.YOUSSEFDepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.MOHAMEDABBASDepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.MOHAMED S.AMARDepartment of General Surgery, Faculty of Medicine, Menoufia University, Shebin-
El-Kom, Menoufia, Egypt.M EMADESMATDepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.HESHAM A.ABDELHAMIDDepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.HISHAMABDELAZIZDepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.AMRMOSTAFADepartment of General Surgery, Theodor Bilharz Research Institute,
Imbaba P.O.B. 30. Giza, Egypt.Journal Article20200114Intraoperative cholangiogram (IOC) is routinely used during cholecystectomy (either open or laparoscopic) in cases of cholelithiasis with history of obstructive jaundice (OJ). It is performed in such cases with no preoperative imaging that confirms the patency of biliary tree and during exploration of common bile duct. Intraoperative ultrasound (IOUS) has recently become a visualizing tool for anatomy and pathology of biliary tree which is safe, faster and less invasive compared to IOC. This is a prospective controlled randomized trial, conducted on 60 patients undergoing laparoscopic cholecystectomy for cholelithiasis with history of OJ, with no present OJ. It was held in the surgery department in Faculty of Medicine, Menoufia University and Theodor Bilharz Research Institute, from 2014 to 2017. Each patient underwent IOUS then, IOC during laparoscopic cholecystectomy. The study compared between both imaging techniques regarding procedure time, visualization of biliary tree, detection of any common bile duct (CBD) stone, biliary or vascular anomalies, and intra-or postoperative complications in the form of obstructive jaundice or iatrogenic biliary injury. The results showed a significant difference in time of the procedure with a mean time (min) of 9.60±1.224 (3-25) in IOUS and 14.391±1.356 (5-30) in IOC. Mean CBD diameter (mm) is 5.280±0.6957 (2.8-18) in IOUS & 7.010±0.7162 (3.5-22) in IOC without significant differences. IOUS has 100% sensitivity and 96.7% specificity while IOC has 100% sensitivity and 100% specificity. A case with vascular anomaly and one with duct anomaly were detected by IOUS. The vascular anomaly could not be detected with IOC. There was no post-operative complication in the form of missed CBD stones or bile leakage.https://jesp.journals.ekb.eg/article_68199_1b0e4c01afd56c567ffb798a5eca213c.pdf