The Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201TRANSMISSION ELECTRON MICROSCOPY OF THE BODY WALL OF PORRORCHIS INDICUS (ACANTHOCEPHALA) WITH SPECIAL REFERENCE TO LEMNISCI47948668004ENHEWAYDAH E.ABOU SHAFEEYDepartment of Zoology, Faculty of Science, Tanta University, Egypt.Journal Article20200112The study reported the ultrastructure features of the different tegumental layers, musculature and lemnisci of Porrorchis indicus (Das, 1957) Schmidt and Kuntz, 1967 (Acanthocephala, Plagiorhynchidae) from the Egyptian Centropus senegalensis aegyptius from Sharkia Governorate. Different semi-thin and ultrathin sections of the middle portion of the preasomal part of P. indicus were cut using transmission electron microscopy (TEM). The results show that the body wall is formed of five successive layers; epicuticle, striped, felt, radial and basal layer, the later rests on basement membrane (basal lamina) which is followed by a layer of circularly oriented muscle fibers. Peripherally, body wall is covered by a very thin glycocalex.https://jesp.journals.ekb.eg/article_68004_0d8bbd3ac2e657fd21028ce11027725a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201HISTOPATHOLOGICAL AND ULTRASTRUCTURAL STUDIES ON CESTODE PARASITES INFECTING DOVES (STREPTOPELIA SENEGALENSIS) FROM EGYPT4874926800510.21608/jesp.2019.68005ENSHEREEN A.FAHMYDepartment of Zoology, Faculty of Science, Damietta University, Egypt.Journal Article20200112Birds live in open environment carry a great risk of parasitic infections. The most important of these parasites are pathogenic cestodes. In February 2016, a total of 10 laughing dove (Streptopelia senegalensis) were examined grossly and microscopically. This study estimated ultrastructural features and histopathological impacts of 27 cestodes<br />parasites isolated from the intestine of laughing dove (S. senegalensis) collected from Damietta Governorate. Data on morphology, hosts and infection intensity of the parasites were provided. The intestine was opened longitudinally examined looking for helminthes which examined by using both light and electron microscopy for identification. Fine morphological criteria of cestodes in the present study are shown by using both light and electron microscopy. Microtriches, the surface features among cestodes comprised two basic types that actually play in the lives of cestodes and an integral role in the formation of certain "hard" structure in cestodes. At present some surface features "microtriches" are illustrated through scanning electron microscopy. Tissue samples were also taken for<br />histopathological examination.https://jesp.journals.ekb.eg/article_68005_499d6df33b98c84339a07a2addd6d440.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201DESCRIPTION OF CESTODES INFECTING DOMESTICATED PIGEON (COLUMBA LIVIA DOMESTICA) IN EGYPT WITH SPECIAL REFERENCE TO THE MOLECULAR CHARACTERIZATION OF RAILLIETINA SPP.4935046805010.21608/jesp.2019.68050ENMARWASAFI-ELDINDepartment of Zoology, Faculty of Science, Ain Shams University, 11566, Egypt.HODA A.TAHADepartment of Zoology, Faculty of Science, Ain Shams University, 11566, Egypt.AMEEN A.ASHOURDepartment of Zoology, Faculty of Science, Ain Shams University, 11566, Egypt.Journal Article20200113This study investigated the prevalence and the specific identity of cestode parasites isolated from the digestive tract of domestic pigeon (Columba livia domestica) in Cairo, Egypt. Out of 246 pigeons, 61 (24.79%) were infected by one or more species. Eight helminthes species were recorded; three nematodes; Ascaridia galli, A. columbae and Heterakis gallinarum and five cestodes; Raillietina echinobothrida, R. tetragona, R. sp., Cotugnia digonopora and Choanotaenia<br />infundibulum. No trematodes were recorded. The morphological observations, using light microscope, were employed for precise identification of cestodes with particular attention to molecular identity of Raillietina spp. using two molecular techniques: RAPD-PCR and SSCP-PCR.https://jesp.journals.ekb.eg/article_68050_a531f78c485214ac832be87798021372.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201ULTRASTRUCTURAL STUDY OF SPERMIOGENESIS AND SPERMATOZOON OF PORRORCHIS INDICUS (DAS, 1957) SCHMIDT AND KUNTZ, 1967 (ACANTHOCEPHALA, PALAEACANTHOCEPHALA, PLAGIORHYNCHIDAE) FROM THE EGYPTIAN CENTROPUS SENEGALENSIS AEGYPTIUS (AVES: CUCULIDAE)5055166805310.21608/jesp.2019.68053ENHEWAYDAH E.ABOU SHAFEEYDepartment of Zoology, Faculty of Science, Tanta University, Egypt.AZZA A.TAELEBDepartment of Zoology, Faculty of Science, Zagazig University, Egypt.SALWA Z.ARAFADepartment of Zoology, Faculty of Science, Zagazig University, Egypt.Journal Article20200113Porrorchis indicus is an endoparasite of the Egyptian birds, Centropus Senegalensis aegyptius. This parasite causes damage to the gut wall of the infected birds. Spermiogenesis and sperm ultrastructure have been widely used in the phylogeny of parasitic platyhelminthic and acanthocephalan worms. However, there are limited ultrastructural data available on spermiogenesis and the spermatozoon on the acanthocephalan family, plagiorhynchidae to which P. indicus belongs. The present study investigated the ultrastructure features of the different stages of P. indicus spermiogenesis. The morphology and anatomy of the spermatozoa was described in comparison with the previous reports. Different ultrathin sections of the body of P. indicus (testes and vas deferens) were cut using transmission electron microscopy (TEM) to describe the different stages of the spermiogenesis as well as both morphology and anatomy of spermatozoa. The results showed that the primary spermatocystes of P. indicus was rounded shape with large rounded nuclei and high nucleocytoplasmic ratio. The secondary spermatocystes showed nearly ovoid shape; each possessed a small nucleus and numerous mitochondria. The early spermatid possessed a large nucleus and contained a single centriole. The early spermatid showed a posterior bulb in which the centriole had a flagellum with 9+0 pattern surrounded by a plasma membrane it changed into 9+2 pattern. The cytoplasmic canal is characterized by a ring form which appeared around the axoneme. The anterior extremity of the spermatozoa showed the presence of the centriolar derivative that had three different stages. The first is made of nine peripheral elements. The second is of nine peripheral and one central element. The third showed nine peripheral and two central elements. The centriolar derivative is followed by a flagellum with a 9+2 pattern. In mature spermatozoa, protein granules different sizes, numbers and shapes were recognized.https://jesp.journals.ekb.eg/article_68053_6b51b5db6eb920e6d32ee10be6344d94.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201STRONGYLOIDES STERCORALIS AND CANCER5175286805410.21608/jesp.2019.68054ENOSAMA SOBHYZAKYMilitary Medical Academy, Cairo 11291, Egypt.ABEER ABDELRAZIKALYMilitary Medical Academy, Cairo 11291, Egypt.TOSSON A.MORSYFaculty of Medicine, Ain Shams
University, Cairo 115662, Egypt.Journal Article20200113Strongyloidiasis is caused by infection with Strongyloides stercoralis. Manifestations of infection can range from asymptomatic eosinophilia in immunocompetent host to disseminated disease with septic shock in the immunocompromised host. Strongyloidiasis is endemic in tropical and subtropical regions and occurs sporadically in temperate areas. Burden of adult worms in infected humans can increase substantially via autoinfection. Among immunocompromised hosts, autoinfection can lead to hyperinfection syndrome where there is massive dissemination of filariform larvae to the lungs, liver, heart, central nervous system, and endocrine glands. Most infected patients do not experience prominent symptoms. The most common manifestations are mild waxing and waning gastro-intestinal, cutaneous, or pulmonary symptoms that persist for years; others simply have eosinophilia in the absence of symptoms.https://jesp.journals.ekb.eg/article_68054_f4454ee167512826cb612b7306d18b6a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201AMELIORATIVE EFFECT OF CALOTROPIS PROCERA AND/OR PRAZIQUANTEL ON SCHISTOSOMA MANSONI INFECTED MICE5295386805510.21608/jesp.2019.68055ENAHMED H.NIGMFaculty of Science, Ain Shams University, Cairo 11566, Egypt.MARWAABOU ELDAHABFaculty of Science, Ain Shams University, Cairo 11566, Egypt.LOTFIA M.KHALILFaculty of Education, El
Zawia University, Libya.RAHMA B. M.JUROUDFaculty of Science, El Mergab University, Libya.AHMAD M.AZZAMTheodor
Bilharz Research Institute, Egypt.HODA A.TAHAFaculty of Science, Ain Shams University, Cairo 11566, Egypt.MOHAMMAD I.SOLIMANFaculty of Science, Ain Shams University, Cairo 11566, Egypt.Journal Article20200113Seventy albino mice were infected with~ 80 S. mansoni cercariae and were classified into: GI: infected non-treated group, GII: treated with C. procera alcoholic extract after infection (therapeutic), GIII: treated with C. procera aqueous extract after infection (therapeutic), GIV: treated with C. procera alcoholic extract before infection (prophylactic alcoholic), GV: treated with C. procera aqueous extract before infection (prophylactic aqueous), GVI: treated with praziquantel, treated group with praziquantel combined with aqueous extract of C. procera and G.VII: infected and treated with half dose of Praziquantel combined with aqueous plant extract. Seven weeks post infection, all mice were autopsied, and livers and ilea were parasitological examined (Tissue egg load) and histological assessments (Number & size of hepatic granulomas). Schistosomes recovered from all groups were processed to calculate total worm burdens. The results showed that mice treated with C. procera alcoholic and aqueous extracts showed more significant reduction in total worm burden (43.7% & 46.4%), total egg tissue load (60% & 50.7%) and number of hepatic granuloma (45.9% & 55.5%) than mice in prophylactic aqueous or alcoholic groups (38.3% & 36%, 27.2% & 44%, 25.6% & 39%, respectively). Female worms<br />recovered from mice of aqueous or alcoholic treated groups showed less fecundity than those recovered from mice of prophylactic groups (1475±181 & 2821±200, corresponding to 3674±1447 & 3023±709, respectively). Mice treated with praziquantel or treated with praziquantel combined with C. procera aqueous extract showed the highest reduction in total tissue egg load (86.4% & 97.4%, respectively). Mice treated with praziquantel combined with C. procera aqueous extract showed higher reduction in total egg tissue (97.4%), number and size of hepatic granuloma (72.3% & 31.4%) than those treated with praziquantel (86.4%, 55.5% &10.8%, respectively).https://jesp.journals.ekb.eg/article_68055_ddd8d81dca46dc1b90fa819808304501.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201EVALUATION OF THE CARD AGGLUTINATION TEST (CATT/T. EVANSI) IN COMPARISON WITH PCR FOR DETECTION OF TRYPANOSOMA EVANSI INFECTION IN DONKEYS IN EGYPT5395426805610.21608/jesp.2019.68056ENAHMEDABDEL-GAWADDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo University,
P.O. Box 12211, Giza, Egypt.AHMEDNASSARDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo University,
P.O. Box 12211, Giza, Egypt.AZZA BABDEL-WAHADepartment of Parasitology, Faculty of Veterinary Medicine, Cairo University,
P.O. Box 12211, Giza, Egypt.Journal Article20200113This study was initiated to evaluated CATT test in comparison with PCR for detection of Trypanosoma evansi infection in donkeys in Egypt. Six donkeys free from T. evansi were inoculated with T. evansi strain (Egypt CD2) which had been isolated from naturally infected horse. Parasitological examination of the donkeys revealed a prepatent period of T. evansi 1- 4 DPI. Two blood samples (heparinized and plain) were taken from each donkey every 4 days starting from day 0 until the end of the experiment for serological examination (CATT/ T. evansi). Body temperature was also estimated. Serological examination of the experimentally inoculated donkeys' no.1, 2, 4 and 5 showed that seroconversion occurred on 12 DPI and remained until death of animals. Other experiment, blood samples from 416 donkeys collected randomly from different governorates of Egypt, they were tested parasitologically (stained blood smear, mice inoculation (MI) and microhaematocrit centrifugation technique (MHCT) and serologically by using CATT/ T. evansi for diagnosis T. evansi infection. From the previous animals, 26 parasitologically negative donkeys were used for comparison between the results<br />obtained by CATT/T. evansi & PCR techniques. Of 26 parasitologically negative samples, 9 (34.6%) & 14(53.85%) samples were found positive by using CATT and PCR techniques respectively. Despite of the simplicity and low-coast of CATT test, it is recommended to use it in combination with PCR technique for accurate and reliable results.https://jesp.journals.ekb.eg/article_68056_a94bcc44fc8f59faec87ed8d28a34173.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201ROSS RIVER VIRUS IS A MOSQUITO-TRANSMITTED ALPHAVIRUS: IS IT THREATENED TO ARAB COUNTRIES INCLUDING EGYPT?5435506805710.21608/jesp.2019.68057ENNAGWA ZEIN EL ABDEENALYMilitary Medical Academy, Cairo1129, Egypt.NAHLA M.SHOUKRYFaculty of Science, Suez University, Egypt.TOSSON A.MORSYFaculty of Medicine, Ain-Shams University, Cairo 11566, Egypt.Journal Article20200113Ross River virus (RRV) is mosquito-borne arboviral infection that causes fever, polyarthritis, and rash. First described in northern Australia and then, widely throughout Australia and many western South Pacific islands. It is common in the tropical coastal regions of northern Australia. RRV can be transmitted by, Aedes aegypti, Ae. camptorhynchus, Ae. vigilax, and Culex annulirostris. Kangaroos, wallabies and horses are principal vertebrate hosts. Symptoms frequently occur together but may occur in single. Arthritis in wrists, knees, ankles, and finger joints is common and frequently symmetric. Rash is usually maculopapular involving limbs and trunk. Arthralgia may persist for up to 3 months. Fatigue is the commonest symptom. Diagnosis is by hemagglutination inhibition (HI) antibody and ELISA. A fourfold rise in HI titer is diagnostic; ≥1:1280 levels is recent infection. RRV-specific IgM persists for months after acute infection. Treatment is supportive care, analgesics, and non-steroidal anti-inflammatory drugs. Feasible preventive measure is avoidance of mosquito bites. Helpful interventions include use of window and door screens, mosquito repellants, and light colored clothing. Over the long-term, drainage of mosquito breeding areas in endemic areas may be helpful to control infection.https://jesp.journals.ekb.eg/article_68057_b5aaa70190de63d9be299915fbfa2794.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201EVALUATION OF NANO-GRAPHENE BASED SANDWICH AND DOT-ELISA AS PROMISING TECHNIQUES FOR DIAGNOSIS OF HUMAN INTESTINAL GIARDIASIS5515626805810.21608/jesp.2019.68058ENFATMA M.EL-LESSYDepartment of Medical Parasitology, Faculty of Medicine for Girls, Al-Azhar
University, Cairo, Egypt.KAREMAN M.ZEKRYDepartment of Medical Parasitology, Faculty of Medicine for Girls, Al-Azhar
University, Cairo, Egypt.AISHA T.HASANDepartment of Medical Parasitology, Faculty of Medicine for Girls, Al-Azhar
University, Cairo, Egypt.IBRAHIM R.ALYDepartment of Parasitology, Theodore Bilharz Research Institute, Imbaba P.O. Box 30, Giza, Egypt.Journal Article20200113Giardia intestinalis nowadays is recognized as the most common parasitological cause of diarrhea, with 280 million infections per year. Microscopic examination of faecal samples has the advantage of low cost and the ability to simultaneously identify other parasitic infections. However, analysis of single stool sample and the skill of the microscopist can affect the accuracy of detection. As an attempt to improve the sensitivity of laboratory diagnosis of giardiasis, the present study aimed to evaluate the diagnostic performance of different parasitological techniques (Mini Parasep, MIFC and direct smear) and to evaluate a novel antigen capture immunoassays based on IgG polyclonal antibody conjugated with nanoparticles (Nano graphene based Sandwich ELISA and Dot-ELISA) for detection of Giardia antigen in stool samples. A total of 96 human stool samples were collected and classified into three groups according to stool examination results, (GI), 61 Giardia infected patients, (GII), 20 samples collected from patients infected with other parasites and (G III), 15 healthy individuals (negative control). In the current study, Giardia antigen detection was carried out by several steps including preparation of Giardia antigen, production, purification and labeling of rabbit anti-Giardia IgG polyclonal<br />antibodies. The Mini Parasep was the best followed by MIFC and direct smear. The study demonstrated that Nano Sandwich ELISA was higher than Traditional Sandwich ELISA regarding sensitivity, PPV, NPV and diagnostic accuracy with statistically significant difference between them, while specificity in Traditional Sandwich ELISA was higher than Nano Sandwich ELISA. Dot ELISA and Nano Dot ELISA had the same sensitivity, while Dot ELISA was higher than Nano Dot<br />ELISA regarding specificity, PPV, NPV and diagnostic accuracy without significant difference. This means that the use of graphene nanoparticles improved the diagnostic testing of human giardiasis.https://jesp.journals.ekb.eg/article_68058_0255b2b3ab34bcb2a08978bc8867a374.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201METARADIOPHRYA EGYPTIACA N.SP. AN ASTOMATOUS CILIATE FROM THE ALIMENTARY CANAL OF ALLOLOBOPHORA CALIGINOSA (ANNELIDA: OLIGOCHAETA).5635706805910.21608/jesp.2019.68059ENAHMED HAMDYNIGMDepartment of Zoology, Faculty of Science, Ain Shams University, Cairo 11566,
Egypt.Journal Article20200113In this study, 639 of Allolobophora caliginosa were collected from different regions of Nile Delta of Egypt. Metaradiophrya egyptiaca n. sp. was recorded in intestinal worms from Bajour (Monoufia Governorate) only. Ciliate species ranged in size from 100-137μm (125.7±9.9μm) in length & 57-88μm (72.5±10.7) in width. Macronucleus is long, band-shape, varied between 71- 106μm (92.8±10.2μm) in length x 5.6-10.3μm (7.3±1.5μm) in width. Ciliate has 2 rows of contractile vacuoles on both side of macronucleus, each row of 4-6 vacuoles. There were 75-84 kineties on body dorsal side and 56-60 kineties on ventral one. The 58 ectoplasmic fibers restricted in anterior ventral depressed region, from which 12-13 ones end on ectoplasm left clear area and 9 fibers from distal end of the right shaft.https://jesp.journals.ekb.eg/article_68059_b5cba31bbad0b1eba6a1c31898abf323.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201ASSESSMENT OF OCCUPATIONAL HAZARDS AMONG NURSING STAFF INSIDE INTENSIVE CARE UNITS IN A MILITARY MEDICAL COMPLEX5715786806010.21608/jesp.2019.68060ENNASHWA ALSAYEDIBRAHIMMilitary Medical Academy, Cairo11291Journal Article20200113This study assessed occupational hazards among nursing staff in intensive care units in a Military Hospital. The study sample consisted of 70 nurses working in ICUs during the study time. A descriptive cross sectional research design was used in carrying out the study. The study conducted in the intensive care units at El Galaa Military Medical Complex. Data collection tool was questionnaire sheet of two parts, socio- demographic characteristics of nurses and risk assessment questionnaire. The results showed that exposure to stress and environmental risk were highest at oncology ICU, exposure to biological risks gave the highest percentage in internal medicine ICU and exposure to radiation was the highest in neurology ICUhttps://jesp.journals.ekb.eg/article_68060_7ca43efdccd03f11c41d21f2bbfe5763.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201TOLERANCE OF HETERACRIS LITTORALIS (ORTHOPTERA: ACRIDIDAE) TO THE TOXIC PLANT, NERIUM OLEANDER (APOCYNACEA)5795876806110.21608/jesp.2019.68061ENMOHAMED MOHAMEDELSHAZLYDepartment of Entomology1, Faculty of Science, Cairo University, Giza P.O. Box
12613, Egypt.NIRVINA ABDEL-RAOUFGHAZAWYDepartment of Entomology1, Faculty of Science, Cairo University, Giza P.O. Box
12613, Egypt.KHALED MOHAMEDABDEL RAHMANDepartment of Entomology1, Faculty of Science, Cairo University, Giza P.O. Box
12613, Egypt.GHADA MOHAMEDELBASSIONYDepartment of Entomology1, Faculty of Science, Cairo University, Giza P.O. Box
12613, Egypt.MAMDOUH IBRAHIMNASSARDepartment of Entomology1, Faculty of Science, Cairo University, Giza P.O. Box
12613, Egypt.Journal Article20200113Nerium oleander (Apocynacea) is a highly toxic plant that has never been reported to be selected as a food or even as a resting place by any acridid species. The study tested the competence of Heteracrislittoralis (Orthoptera: Acrididae) to tolerate and survive on this plantfor considerable periods, and to resume normal capacity of development and reproduction when favourable food plant became available. Nymphal survival and development were followed by monitoring three cohorts of freshly hatched hoppers. The first cohort fed oleander leaves; the second one (control) fed clover; while the third cohort fed oleander leaves for 35 days, and then switched to clover. Age specific survivorship and reproductive parameters of the adults were followed by monitoring three cohorts of newly fledged adults fed on the same<br />patterns. Moulting was suspended in nymphs fed oleander; when food plant was switched to clover, the insects resumed normal development and reached the adult stage. Adult cohort reared on N. oleander exhibited a normal age specific survivorship curve, but ovarian development was suspended. Suspension of ovarian development by N. oleander was accompanied by very low indices of food consumption and conversion of digested food into body substance.<br />But, after switching to normal food, clover, the insects resumed normal ovarian development with significant prolonged longevity, and the fecundity was approximately equal to the same parameter in the control cohort. This reflects a strong evolutionary biotic potential against the selective power of plant defense.https://jesp.journals.ekb.eg/article_68061_a1269b70796a0f2681eb85b35c41337c.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201EFFECT OF THE EGYPTIAN SAHARA SAND VIPER VENOM ON HUMAN BREAST CANCER: AN IN VITRO STUDY5895986806210.21608/jesp.2019.68062ENABIR A.EL-FIKYAnti-venom Research, (VACSERA), Dokki, Egypt.AHMEDELDOUSOUKYFaculty of Biotechnology,
October University for Modern Science and Arts University, 6th of October, Egypt.DINA M.ELKHASHABDepartment of Clinical Pathology, National Cancer Institute, Cairo University, Egypt.Journal Article20200113Cancer is a major health problem which is considered as one of the most reasons of death worldwide. Breast cancer is a second cause of women death and estimated to have 2.1 million new cases. Traditional treatment of cancer based on different clinical approaches is included surgery, chemotherapy, radio therapy, hormone therapy and combination therapy which affect both cancer and normal cells. The present study investigated the anticancer potential of Cerastes vipera venom in comparison with Cisplatin drug on MDA-MB-231 breast cancer cell line. Cytotoxicity activity of tested material was evaluated using MTT assay. Cell cycle arrest was performed using propidium iodine DNA staining. Apoptotic detection was determined using annexin V-FTIC and for the detection of the genes Caspase 3, Caspase 8 & Caspase 9 were<br />determined by ELISA. The C. vipera venom, Cisplatin and PLA2 had IC50 as 3.4, 10.3, 35.1 μg/mL with MDA-MB-231 cell line respectively. Cell cycle has arrest of cells in G2/M of C. vipera venom and PLA2. Tested venoms have apoptotic effect against MDA-MB-231 cell line through elevation of Caspase 3, Caspase 8 & Caspase 9 genes.https://jesp.journals.ekb.eg/article_68062_8f2a2adb0ae0e4d16a6a56a845eabeb8.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201IN VITRO ANTIHELMINTHIC ACTIVITY OF ETHANOL ZINGIBER OFFICINALE EXTRACT ON FASCIOLA GIGANTICA IN COMPARISON TO TRICLABENDAZOLE5996106806310.21608/jesp.2019.68063ENFAWZIA H.TOULAHDepartment of Biological Sciences, University of Jeddah, Jeddah, Saudi Arabia.SAFINAZ J.ASHOORDepartment of Biological Sciences, University of Jeddah, Jeddah, Saudi Arabia.MAJED H.WAKIDFaculty of Applied
Medical Sciences, King Abdul Aziz University, Jeddah, Saudi Arabia.MONA R.ALSHATHLYDepartment of Biological
Sciences, Northern Border University, Arar, Saudi Arabia.Journal Article20200113Fascioliasis, caused by Fasciola hepatica and F. gigantica, is considered one of the most important helminthes diseases among both humans and animals. The use of triclabendazole (TCBZ) as the only antihelminthic drug against fascioliasis faced recent problems being of many side effects and development of the drug resistance by the parasites. Given the widespread use of ginger (Zingiber officinale) in many traditional medicines and the various metabolic properties, this study aims to investigate the in vitro antihelminthic activity of Z. officinale ethanol extracts on F. gigantica in comparison to TCBZ. Fifty-four live adult F. gigantica worms were divided into nine groups of six in each, including positive control (G1), negative control (G2), triclabendazole sulfoxide (TCBZSO) of 20μg/ml (G3), ginger extract of 5, 25 and 50 mg/ml (G4, G5 and G6, respectively) and combined TCBZSO with ginger extract of 5, 25 and 50 mg/ml (G7, G8 and G9, respectively). The worm motility scores, survival index and histological examination were utilized to further analyze the effect of treatments on the worms' morphology. Results indicated a marked decrease in worms' motility treated with<br />ginger extracts compared to TCBZSO group. The observed decrease was positively correlated to both time and concentration. Histological examination showed that a higher concentration of ginger extract alone or in combination with TCBZSO caused severe tegumental alterations, more than those observed in TCBZSO treatment alone. In conclusion, the results strongly confirm the plausible development of ginger-based antihelminthic drug against F. gigantica infection.https://jesp.journals.ekb.eg/article_68063_c952744f7ff54ccd07087b07530c85d1.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201CRYPTOSPORIDIOSIS PROVOKING DIARRHEA AMONG INFANTS, TODDLERS AND PRESCHOOL CHILDREN6116176806410.21608/jesp.2019.68064ENMARWA A.GEIETHDepartments of Medical Parasitology, Faculty of Medicine, Beni-Suef
University, Egypt.ESSAD M.MONAZEEEDepartment of Pediatrics, Faculty of Medicine, Al Azhar University, Cairo, Egypt.MOHAMED A.ELMARAGHYPediatrics, Faculty of Medicine, Beni-Suef
University, Egypt.AHMED H.ABDEL MOTAMEDDepartment of Pediatrics, Beni-Suef General Hospital, Beni-Suef University,
Egypt.HALA M.EL-ASKARYDepartments of Medical Parasitology, Beni-Suef University, Egypt.Journal Article20200113Cryptosporidium intestinal coccidian is a preeminent account for infectious diarrhea with reference to children. Oocyst may escape ordinary microscopic analysis or immune detection; molecular techniques are of convenient use. This study intent to detect cryptosporidiosis infection among children, and debates risks for infection. A total of 189 children with age range from under one to five complaining of gastrointestinal manifestations were examined coproscopically, by acid fast stain, ELISA and nested PCR (nPCR) for Cryptosporidium from June to September. Infection was proved by nPCR among 13 (6.8%) samples. ELISA sensitivity merit stool staining (77%, 53.8%, respectively), however all positive samples by microscopy were settled by nPCR. Age groups (P = 0.02) were statistically associated with infection. Source of<br />water (OR=0.069, 95% CI= 0.004/1.165, P = 0.01) and presence of undigested food particles on microscopy (OR= 1.058, 95% CI= 0.521/6.154, P = 0.02) were risks for infections. No other variables were predicated risks. Infection was potent among preschool children (46.2%), toddlers (38.5%) and then infants (15.4%). Cryptosporidiosis should be doubtful among children with diarrhea during preschool age. Source of water for ordinary drinking activities or for infants<br />with non-exclusive breast or complementary feeding should be considered as hazards aspects for acquiring Cryptosporidium.https://jesp.journals.ekb.eg/article_68064_13c7d500204957a55fc1ab2a85fb4d06.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201PREVALENCE OF GASTROINTESTINAL PARASITES AND ITS PREDICTORS AMONG RURAL EGYPTIAN SCHOOL CHILDREN6196306806510.21608/jesp.2019.68065ENDOAA A.YONESDepartment of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.RAGAA AOTHMANDepartment of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.TASNEEM M.HASSANDepartment of Medical Parasitology, Faculty of Medicine, Assiut University, EgyptSAFAA A. M.KOTBDepartment of
Community Health Nursing, Faculty of Nursing, Assiut University, Egypt.ASMAA G.MOHAMEDDepartment of
Community Health Nursing, Faculty of Nursing, Assiut University, Egypt.Journal Article20200113The development of suitable control measures for various intestinal parasitic infections (IPIs) requests essential epidemiological information on their prevalence. The present study was conducted to determine the prevalence of IPIs among school children living in a rural setting in Upper Egypt and to identify the associated risk factors to these infections. A Descriptive crosssectional study included 630 randomly selected students enrolled in primary and preparatory schools aged from 6 to 17 years old from rural Assiut Governorate. The data were collected using a structured questionnaire. A laboratory stool examination was performed. The overall prevalence of IPIs was 56.3 %. The parasites were Ascaris lumbricoides (11.4%), Giardia lamblia (10.0%), Entamoeba coli (9.7%), Ancylostoma duodenal (8.7%), Entamoeba histolytica/ dispar (8.1%), Cryptosporidium parvum (3.3%), Blastocystis hominis (2.4%), Enterobius vermicularis (1.7%) and Hymenolepis nana (1.1%). A single parasite was in 64.8% of children. 23.1% & 12.1% had double and multiple parasites respectively.https://jesp.journals.ekb.eg/article_68065_b132c47034757a773f27cc31746afc73.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201HISTOPATHOLOGICAL STUDY ON MICE EXPERIMENTALLY INFECTED WITH S. MANSONI AND TREATED BY NEEM AND MIRAZID6316426806610.21608/jesp.2019.68066ENHANAN TAHERHAMZADepartment of Biology, College of Science, Jouf University, P.O. Box: 2014,
Sakaka, Saudi Arabia.+Department of Zoology, Faculty of Science for Girls, Al-Azhar
University, Cairo, EgyptFAYEZ M.SHALDOUMDepartment of Biology, College of Science, Jouf University, P.O. Box: 2014,
Sakaka, Saudi Arabia.+Department of Zoology, Faculty of Science for Boys, Cairo, Egypt.WAFAA FAYEZABDEL HAMEDDepartment of Environmental and Biological Sciences, Faculty of Home Economics4,
Al-Azhar University, Cairo, Egypt.Journal Article20200113Generally, the parasitological markers showed that Neem (Azadirachta indica) and Mirazid (Commiphora molmol, MZ) were effective in reducing worms and eggs of Schistosoma mansoni. This study aimed to detect the histopathological improvement accompanied with using Neem and Mirazid to treat schistosomiasis. A total of 120 Swiss Albino mice were categorized into 10 groups: 5 groups infected with S. mansoni and either treated with only Neem (400 mg/kg), only MZ (500mg/kg), a combination of Neem and MZ, praziquantel (PZQ, 200 mg/kg) or not treated. Other 5 control groups were not infected but acquired the same treated doses as the above groups. The study was done in Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt. Hematoxylin eosin and Masson’s trichrome stain stained sections<br />were used to examine the histopathological changes in the intestine and liver of mice. The results showed marked decrease of liver fibrosis in groups of mice treated with neem and/or MZ. This was ensured by a significant diminution in number and diameters of granulomas, reduction in their fibrotic content accompanied with a reduction in total area of infection as compared to infected mice.https://jesp.journals.ekb.eg/article_68066_8bf82e1ebcd2f05a44831937ddedd9f4.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201USEFULNESS OF CD44 LEVEL AS A BIOMARKER IN DIAGNOSIS OF ACUTE LIVER REJECTION6436526807010.21608/jesp.2019.68070ENESAM M.BAYOMYDepartment of Internal Medicine and Gastroentrology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.TAREK M.YOUSEFDepartment of Internal Medicine and Gastroentrology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.KHALID M.MANSOURDepartment of Internal Medicine and Gastroentrology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.DOAA Z.ZAKYDepartment of Tropical
Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.AHMED M.EL GHANDOURDepartment of Internal Medicine and Gastroentrology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.MOHAMED B.AHMEDDepartment of General Surgery and Liver Transplantation,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.Journal Article20200113The incidence of acute and chronic rejection has declined with improvement of immunosuppression regimens in liver transplant recipients. The CD44 protein belongs to a large family of type I Trans membrane glycoproteins and expressed on the surface of most vertebrate cells and is an important receptor for the components of extracellular matrix. The study evaluated if the serum level of CD44 had a value in diagnosis of acute rejection depending on proved rejection by liver biopsy. The patients were recruited from ASCOT from May 2017 to December 2018. They were 20 adults with Post LDLT with elevated liver function tests and were reviewed. The results showed that serum level of CD44 was significantly lower in the rejection group in our study in patient with post liver transplantation. There was highly significant difference between controls & patients \as regards serum level of CD44. Negative correlation between serum levels of CD44, WBCs & AST. There was highly significant difference between patients with different pathological findings as regards serum level of CD44. The best cut off point for serum level of CD44 as a marker of rejection was found ≤ 44ng/ml with sensitivity of 85% and specificity of 95% in diagnosis of acute cellular rejection.https://jesp.journals.ekb.eg/article_68070_7f47c59697a0ac6f45aa6b542f5193e3.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201EVALUATION OF DONATED BLOOD FOR TOXOPLASMOSIS USING ELISA AND PCR IN FAYOUM GOVERNORATE6536606807110.21608/jesp.2019.68071ENRAMY WAHBAHENINDepartment of Parasitology, Parasitology, Faculty of medicine, Fayoum University,
Egypt.Journal Article20200113Toxoplasma gondii is an intracellular zoonotic parasite that affects more than a third of the world population. Among many modes of transmission, blood transfusion is a potential risk for transmitting toxoplasmosis especially to high risk individuals as immunosuppressed persons and pregnant women. This cross-sectional study evaluated the donated blood for T.<br />gondii infection using ELISA also the puffy coat was separated and PCR for toxoplasma is done. 100 blood samples were collected from blood banks in Fayoum Governorate.https://jesp.journals.ekb.eg/article_68071_7129d4762d90ae97a42c0376f2ab4dab.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201A NON INVASIVE METHOD FOR DIAGNOSIS AND ASSESSMENT OF PORTAL HYPERTENSION IN EGYPTIAN CIRRHOTIC PATIENTS USING PLASMA MALONDIALDEHYDE LEVEL6616686807210.21608/jesp.2019.68072ENAHMED SAMIRALLAMDepartments of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.AYMAN GAMILANWARDepartments of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.RAMY SAMIRGHAITDepartments of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.TAMERABDEL RAHMANDepartment of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.WALAA AHMED YOUSRYKABIELDepartment of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.HAYTHAM M.NASSERDepartment of Radiology, Faculty of Medicine, Ain Shams University, Cairo 11211, Egypt.MOHAMEDHASSANYNational Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.Journal Article20200113Liver cirrhosis is a common disease affecting Egyptian patients. Complications of liver cirrhosis usually start once portal hypertension increases. To the authors’ knowledge, there are no available non-invasive methods for assessment of the severity of portal hypertension. This study evaluated the value of plasma Malondialdehyde (MDA) - a lipid peroxide marker for oxidative stress, as a diagnostic biomarker, to assess severity of Portal Hypertension in Egyptian cirrhotic patients. It is a case-control study conducted on a total of 150 Egyptian patients divided into two groups. GI: 100 patients diagnosed as liver cirrhosis with esophageal varices proved by upper GIT endoscopy. GII: 50 normal controls. Serum MDA was measured by ELISA technique. The results showed that MDA assay between the two groups revealed significant statistical<br />difference between the two studied groups. Correlation between MDA and the clinical parameters in the cirrhotic group showed a significant positive correlation with ascites, Child Pugh score, varices grade, while there was no correlation with sex.https://jesp.journals.ekb.eg/article_68072_6fe2ce89187bd7fe484a1d2ef8391190.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201PREVENTION OF ARTHROPOD-VECTOR OF INFECTIOUS DISEASES BY INSECT REPELLENTS AND OTHER MEASURES6696806807410.21608/jesp.2019.68074ENTOSSON A.MORSYFaculty of Medicine, Ain Shams University, Cairo 115661, Egypt.AYMAN T. A.MORSYConsultant of Tropical
Medicine and Fevers, Egypt.EMAN E.ABDEL-FADILMilitary Medical Academy, Cairo 11291, Egypt.Journal Article20200113The bloodsucking arthropod bite is the cofactor in the transmission of infectious diseases to man and animals. They release with saliva powerful anti-homeostatic and immuno-modulators that favor the replication and the establishment of the infective pathogen. The transmission depend on the close contact of the child with a seropositive mother (or relatives) whose infective saliva is used to relieve itching and scratching at the arthropod bite's sites. Vector-borne diseases<br />are caused by parasites, bacteria or viruses transmitted by the hematophagous arthropods bite (mainly ticks and mosquitoes). The past decades there were emergence of new diseases or re-emergence of existing diseases, due to changes in their epidemiology (i.e. geographical distribution, prevalence, pathogenicity and vector-capacity). Besides, human factors, such as travel with pets, changes in human habitats, social and leisure activities have their input, but climate changes also have a direct impact on arthropod vectors. Vaccinations are up to date for diseases prevalent in the area, but what about diseases beyond vaccination? Wear light-colored, long-sleeved shirts and long trousers, tucked into socks or boots, and the use of insect repellent on exposed skin and clothing to protect from being bitten by mosquitoes, sandflies and/or ticks. This review discussed the vectors, diseases and feasible insecticidal control.https://jesp.journals.ekb.eg/article_68074_a2d9ede80b07803c118d833c8ffb7883.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201OUTCOME OF A LOW-CARBOHYDRATE DIET ON EGYPTIAN PATIENTS WITH NON ALCOHOLIC FATTY LIVER DISEASE6816876807510.21608/jesp.2019.68075ENDOAA Z.ZAKYDepartment of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.KHALID A.MANSOURDepartment of Internal Medicine,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.Journal Article20200113Non-alcoholic steatohepatitis (NASH) is a worldwide health problem and imposes a high cost on healthcare system, and NASH is associated with an unhealthy lifestyle. This study investigated whether low carbohydrate diet intervention physical activity and affect intrahepatic fat content and hepatic enzymes. The current interventional study included participants who were over 18 years with NASH (n= 30) with BMI over 25 kg/m2. The effects of proper nutritional therapy (moderate caloric restriction, low carbohydrate) was tested through personal dietary and physical assessment,<br />parameters of the metabolic syndrome and insulin resistance in addition to liver biochemical and imaging study . The assessment was done at baseline and at 3 months by the same observer. The results showed that after 3 months of intervention, patients had a significant decrease in caloric consumption, weight loss (p < 0.001) and HOMA-IR had significant improvements. Lipid profile after 3 months showed significant reduction including cholesterol and triglycerides<br />levels (p < 0.05). Regarding liver profile significant decrease observed in aminotransferases levels; also there were significant improvements in AST/ALT ratio with decrease in severity of liver steatosis.https://jesp.journals.ekb.eg/article_68075_36f8b0ae556da2916c5b2c0850868faa.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201EFFECT OF CINNAMON AND GINGER METHANOLIC EXTRACTS ON MURINE INTESTINAL CRYPTOSPORIDIOSIS.IN-VIVO EVALUATION6896986807610.21608/jesp.2019.68076ENEMAN MAGDYFAWZYDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.RABAB SAYEDZALATDepartment of Parasitology,HAYAM ELSAIDRASHEDTheodor Bilharz Research Institute, Embaba, Giza and Department of Pathology, Faculty of Medicine, Zagazig University, Egypt.MARWA AHMEDSALAMADepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.AMIRA A.SALEHDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.ENAS FAKHRYABDELHAMEDDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.Journal Article20200113Cryptosporidium species the Apicomplexa protozoan parasites, causing diarrheal disease in man and animals, due to the lack of a licensed vaccine and resistance to the available effective therapy. This study explored the curative and the prophylactic effect of the cinnamon and ginger methanolic extracts separately and combined with nanazoxide on the experimentally infected mice with Cryptosporidium. Ninety Swiss albino mice, weight of 23-25gm, aged 7-8 weeks were divided into nine groups to assess the therapeutic and the prophylactic effect of cinnamon and ginger methanolic extracts separately then combined with nanazoxide. The result was assessed parasitologically, histopathlogically and by TEM. The best efficacy was three weeks post infection in all groups. Therapeutic effect of ginger was better than cinnamon extract, but combined ginger and nanazoxide gave the least oocyst shedding. The prophylactic dose of ginger and cinnamon methanolic extracts showed marked decrease in oocysts shedding and ginger was the best. Histopathological sections showed immune cells infiltration with a decrease in number of tissue parasite that was absent in the prophylactic groups given ginger extract. The results were confirmed by transmission electron microscopy explored a great improvement<br />in the small intestinal brush border of the same groups.https://jesp.journals.ekb.eg/article_68076_341faee9a98d1258a7a60e588742d32f.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201IMPACT OF OBESITY ON SUSTAINED VIROLOGIC RESPONSE TO SOFOSBUVIR BASED REGIMENS IN THE EGYPTIAN CHRONIC HEPATITIS C PATIENTS6997066807710.21608/jesp.2019.68077ENAHMED SAMIRALLAMDepartments of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.AYMAN GAMILANWARDepartments of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.HAYTHAM MOHAMEDNASSERDepartments of Radiology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.WALAA AHMED YOUSRYKABIELDepartments of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.AMIR M.FARIDDepartments of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.Journal Article20200113Hepatitis C virus is one of the flaviviruses that can cause both acute and chronic hepatitis. Chronic HCV infection often follows a progressive course over years that could result in cirrhosis, hepatocellular carcinoma, and possible liver transplantation. Identifying modifiable risk factors that add to progression of the disease in HCV patients aids in choosing treatment approaches and overall disease management. Hepatic fibrosis may occur as a result of obesity, host, virus-mediated factors and hepatic steatosis. Nonetheless, the role of overweight and obesity on hepatitis C progression remains debatable. Nowadays, Direct Acting Antiviral therapy is the cornerstone of treatment of chronic hepatitis C virus infection aiming to eradicate HCV RNA, which is predicted by reaching sustained virologic response (SVR). This prospective observational cross sectional study assessed the impact of obesity on the SVR to Sofosbuvir based therapy in patients with chronic HCV infection. The study included 188 chronic hepatitis C patients eligible for antiviral therapy according to the Egyptian guidelines issued by the National Committee for Control of Viral Hepatitis. G1: 93 obese patients<br />with BMI ≥30 kg/m2 & G2: 95 non obese patients with BMI < 30kg/m2. Each group was subdivided into 3 subgroups; GA received Sofosbuvir & Daclatasvir, GB received Sofosbuvir & Simeprevir while GC received Sofosbuvir & Ledipasvir.<br />The results showed that overall SVR was 96.7% in G1 and 95.7% in G2. There was no association found between stage of fibrosis and SVR, and no significant association reached between obesity and sustained virologic response (SVR) to Sofosbuvir based regimens.https://jesp.journals.ekb.eg/article_68077_12e854f1955d673073e0703d892499c9.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201TWO PORTS VERSUS FOUR PORTS LAPAROSCOPIC CHOLECYSTECTOMY (THE USE OF STRATEGIC EXTRACORPOREAL STITCHES)7077126807910.21608/jesp.2019.68079ENSAMEHELSEBAIEDepartment of General Surgery, Theodor Bilharz Research Institute Hospitals,
Imbaba P. O. B. 30, Giza, Egypt.HESHAMABDELAZIZDepartment of General Surgery, Theodor Bilharz Research Institute Hospitals,
Imbaba P. O. B. 30, Giza, Egypt.AMRMUSTAFADepartment of General Surgery, Theodor Bilharz Research Institute Hospitals,
Imbaba P. O. B. 30, Giza, Egypt.AHMEDABDELAZIZDepartment of General Surgery, Theodor Bilharz Research Institute Hospitals,
Imbaba P. O. B. 30, Giza, Egypt.YASSER A.ELSAYEDMilitary Medical Academy, Cairo 11291, Egypt.MOHAMEDABDELHALIMDepartment of General Surgery, Theodor Bilharz Research Institute Hospitals,
Imbaba P. O. B. 30, Giza, Egypt.Journal Article20200113Four ports laparoscopic cholecystectomy (LC) has been the standard for treatment of the symptomatic cholelithiasis. Herein, the experience of two-ports LC regarding its safety and feasibility was given. This study was conducted in Theodor Bilharz Research Institute (TBRI) in the period from November 2017 till April 2018, as a prospective randomized study including one hundred patients with chronic calcular cholecystitis, which were divided into two equal groups. The study compared between group A (GA) which underwent four ports LC and group B (GB) which underwent two ports LC.<br />The results showed that in GA, no cases were converted to open cholecystectomy. But, in GB only two patients converted to three-port laparoscopic cholecystectomy. There were no intraoperative complications encountered in any patient in both groups. In GA, operative time ranged between 28-62 minutes (mean±44.57) while in GB, operative time ranged between 39-134 minutes (mean±59.93); with hospital stay of three days in both groups. The pain score showed statistically significant differences regarding port-site pain (P value = 0.040). Significant lower analgesic demands were recorded in the 2 ports group. Scar satisfaction showed a marked statistically significant differences regarding the aesthetic results between the two groups (P value = 0.0319).https://jesp.journals.ekb.eg/article_68079_3d792f88283b84442e0bdb51d0b0478b.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201ENTOMOLOGICAL SURVEILLANCE OF AEDES AEGYPTI AND ARBOVIRUSES OUTBREAK OF DENGUE FEVER IN THE RED SEA GOVERNORATE, EGYPT7137186808010.21608/jesp.2019.68080ENAZZA A.MOSTAFAResearch Institute of Medical Entomology, Ministry of Health and Population, Dokki, Giza, Egypt.MOHAMMED E.RASHEDResearch Institute of Medical Entomology, Ministry of Health and Population, Dokki, Giza, Egypt.NOUR ELDIN S.ALYResearch Institute of Medical Entomology, Ministry of Health and Population, Dokki, Giza, Egypt.AHMED H.HASANResearch Institute of Medical Entomology, Ministry of Health and Population, Dokki, Giza, Egypt.MICHEAL W.MIKHAILResearch Institute of Medical Entomology, Ministry of Health and Population, Dokki, Giza, Egypt.Journal Article20200113Red sea Governorate coordinates: N 25o 32' 1'', E 33o 26' 18'' and one of the borders Governorates. The Red Sea Coast length of 1080 km, from the Gulf of Suez, latitude 29 north, to the border of Sudan on latitude 22 north with about 306,000 living in area of about 203,685km2(January 2018), and it is divided into seven cities include Hurghada (capital), Safaga, Al-Kuseer, Ras Ghareb, Marsa Alam, Shalatin, Halaib. Four Cities Hurghada, Safaga, Al-Kuseer, Ras Ghareb were selected for entomological surveys where dengue fever was anticipated to be occurred at November 2017. A total number of 469 houses were participates survived, positive adult houses were 20.47% (96/469), while positive larvae houses were 10.23 % (48/469). The stogmoyia indices for the parameters of house index (HI), container index (CI) and Breteau index (BI) were (10.2, 7.0 &16) respectively. The survey yielded a total number of immature stages of Aedes aegypti of 92.22% (960/1041), or 92.5 % (888/960) for larvae and 7.5% (72/960) for pupa. The immature stages of other species were recorded 7.78% (81/1041), Aedes detritus, Culex pipiens, Cx. antennatus and Cx. perexiguus and represented 86.42% (70/81) for larvae and 13.58% (11/81) for pupa. Adults Ae. aegypti were 20.36% (90/442), but adult mosquito of other species were 79.64% (352/442). Adults Ae. aegypti at Hurghada showed resistant to deltamethrin, cyflothrin and bendiocarb, and suggested resistance to lambda-cyhalothrin, but they were susceptible to malathion. At Safaga City showed resistant for bendiocarb and susceptible to cyflothrin. Larvae of Aedes aegypti at Hurghada City showed resistant to chlorpyrifos, but susceptible to temephos. Also, at Safaga City showed susceptible to temephos insecticide.https://jesp.journals.ekb.eg/article_68080_e7cccc919d389f3e619f30067041302a.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201INFLUENCE OF CONVECTIVE DRYING TYPE ON PHENOLIC COMPOUND COMPONENTS OF THE EGYPTIAN HONEY BEE POLLENS7197256808210.21608/jesp.2019.68082ENEMAD A.NAFEADepartment of Bee Research, Plant Protection Research Institute, ARC, Dokki,
Giza, Egypt.EHAB W.ZIDANDepartment of Bee Research, Plant Protection Research Institute, ARC, Dokki,
Giza, Egypt.DOAA S.FARGHALYDepartment of Bee Research, Faculty of Science, Al -Azhar, University for Girls Branch, Egypt.ASMAA M.FAWZYDepartment of Bee Research, Plant Protection Research Institute, ARC, Dokki,
Giza.+Department of Biology, Faculty of Science, Taibah University, Saudi Arabia.Journal Article20200113The present work determined theefficacy of three drying methods,41oC sun drying (SD), 32oC shadow drying (ShD) and 32, 40 & 50oC incubator (ID) on the percentage of moisture loses and phenolic compounds content of pollen grains throughout 72h. SD & ShD drying bee pollens showed the highest number of phenolic compound after 72h. with total peak of 11388228 & 14374500 respectively. The 32°C, 40°C & 50°C ID of bee pollens showed the highest number of phenolic compounds after 24h.,with total peak of12429414, 12078595 & 12578030 respectively.https://jesp.journals.ekb.eg/article_68082_1523b21b8214218915e0c9e98aacb755.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201CYCLOSPORA INFECTION IN RENAL TRANSPLANT RECIPIENT7277306808310.21608/jesp.2019.68083ENMOUSA A. M.ISMAILDepartment of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.HANAA O.FADLDepartment of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.Journal Article20200113Cyclospora cayetanensis (C. cayetanensis) can cause serious diarrheal illness in immunocompromised patients. The present work aimed to detect C. cayetanensis infection among patients with renal transplantation attending the nephrology unit of Kasr Al-Aini, Faculty of Medicine, Cairo University. A total of 50 stool samples were collected and subjected to direct microscopy to screen for parasitic stages. A modified acid fast staining technique (Kinyoun’s method) was used to detect C. cayetanensis oocysts. Cyclospora oocysts were revealed in 5 (10%) of the stool samples examined. Other parasites detected among the patients included Cryptosporidium parvum 5 (10%) and Blastocystis 15 (30%). 30% of the patients were suffering from diarrhea and or colic. All C. cayetanensis positive cases were presenting with diarrhea.https://jesp.journals.ekb.eg/article_68083_a17ec3135edb83842cf5cfe6d4308c70.pdfThe Egyptian Society of ParasitologyJournal of the Egyptian Society of Parasitology1110-058349320191201MORPHOLOGICAL DESCRIPTION OF TWO DIGENEAN PARASITES, FROM LABRIDAE, AND SIGANIDAE HOSTS OF THE RED SEA IN EGYPT7317376808510.21608/jesp.2019.68085ENZEINAB I.ADAMDepartment of Zoology, Faculty of Science, Cairo University, Egypt.SALWA A.HAMDIDepartment of Zoology, Faculty of Science, Cairo University, Egypt.MANAL F.EL GARHYDepartment of Zoology, Faculty of Science, Cairo University, Egypt.KAREEM S.MORSYDepartment of Zoology, Faculty of Science, Cairo University, Egypt.Journal Article20200113During a parasitological survey on marine fish species inhabiting Hurghada Coasts of the Red Sea, Eighty seven fish specimens of 2 different species were found naturally infected by three different species of digenean parasites, one of them was reported as new host and locality in Egypt. These were Phyllodistomum hoggettae (family: Gorgoderidae) infecting the Arabian mallas Thalassoma klunzingeri (family: Labridae). Body length to width ratio 1:3. Oral sucker opened subterminally. Intestinal bifurcation midway between oral and ventral suckers. Ventral sucker distinctly equal in size to the oral sucker. Testes slightly lobed, oblique, in mid-body; ovary entire, heart shaped, sinistrally posterior to ventral sucker and anterior to testes. Vitelline lobes entire and almost round. Gyliauchen volubilis (family: Gyliauchenidae) infecting the marbled spinefoot Siganus rivulatus (family: Siganidae). Body fleshy, robust, conical, tapered anteriorly, convex dorsally, concave ventrally, with small tail-like protubera-nce postero-dorsal to acetabulum. Prepharynx very long and convoluted, occupied greater part of the anterior body half. Pharynx elongated, prephanyx very coiled and situated close to intestinal bifurcation. Ventral sucker well developed and spherical. Oesophagus very long, wide, longer than body straightened length. Two testes oval, symmetrical, dorsal to ventral sucker, subequal in diameter. Ovary spherical, dextro-submedian between right testis and cirrus sac. Vitelline follicles relatively small, irregular, numerous, extended in lateral fields between testes and mid-oesophageal level, confluent in region of intestinal bifurcation. All of the recovered species were compared with others recorded from different hosts.https://jesp.journals.ekb.eg/article_68085_06e254ccf303652c1d78e5ddd4cb9974.pdf