TY - JOUR ID - 257454 TI - VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES JO - Journal of the Egyptian Society of Parasitology JA - JESP LA - en SN - 1110-0583 AU - NAHAS, ENGY AHMED ABDALLAH AU - AUFY, SAYEDA M. AU - ISMAIL, MOUSA A. M. AU - MOHAMED, REHAM KAMAL AU - EL AKKAD, DINA M. H. AD - Department of Parasitology, Faculty of Medicine, 6th October University AD - Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt Y1 - 2022 PY - 2022 VL - 52 IS - 2 SP - 261 EP - 266 KW - Giardia KW - Cryptosporidium KW - ELISA KW - coproantigen KW - ICT KW - MZN KW - Formol ether concentration DO - 10.21608/jesp.2022.257454 N2 - Giardiasis and cryptosporidiosis are common protozoa causing gastrointestinal troubles with symptomsranged from asymptomatic to long-lasting diarrhea with mal-absorption. The present work wascarried on a total of 96 cases; 62 of them presented with GIT symptoms with or without diarrhea, 50%of them was previously diagnosed as giardiasis or cryptosporidiosis infected and treated 1-2 monthsago, 11 cases were free from GIT symptoms and 23 cases suffered from chronic debilitating disease ascancer or renal disease, some of them have previous history of gastro-intestinal disturbances.The rapid immunochromatographic test (ICT) and copro-ELISA detected G. lamblia and C. parvumantigens in all fecal samples compared to formol ether concentration and Modified Ziehl-Neelsen(MZN) staining technique. The sensitivity of G. lamblia in stool samples was 60%, 98.3% & 96.6%using concentration technique, ICT & ELISA respectively, and specificity was 98.75%, 96.43% &96.43% respectively. The sensitivity of C. parvum with MZN stain and antigen detection by ICT&ELISA was 46.2%, 96.4% & 100% respectively. Specificity of the tests in detecting C. parvum wasbetter than Giardia; 96.4% -100% respectively. All tests have 100% PPV and 80-888% NPV for theimmunological techniques with superiority of ICT over ELISA. UR - https://jesp.journals.ekb.eg/article_257454.html L1 - https://jesp.journals.ekb.eg/article_257454_a40d0e742534e42bbf3033e2b5e79699.pdf ER -