The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
CYTOKINES AND MOTHER SPOROCYSTS IN SUSCEPTIBLE AND RESISTANT BULINUS TRUNCATUS SNAILS INFECTED WITH SCHISTOSOMA HAEMATOBIUM
285
293
EN
ABDEL
HAKIM
SAAD EL-DIN
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
FATHIYA
ALI
GAWISH
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, PO Box 30, Imbaba, Giza, Egypt.
HANAA
MOHAMED
ABU EL EININ
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, PO Box 30, Imbaba, Giza, Egypt.
SHEREEN
MAHFOUZ
MANSOUR
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, PO Box 30, Imbaba, Giza, Egypt.
10.21608/jesp.2014.90379
The presence of immunoreactive interleukin (IL-2), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in addition to the citation of mother sporscytes in cephalopodal musculature in the susceptible and resistance Bulinus truncatus the specific intermediate host for the trematode Schistosoma haematobium were investigated,. Using ELISA tests, Results indicated that the concentration of IL-2-like activity in the susceptible and resistant snails decreased significantly after infection then persisted at low levels until the 4th week post exposure (WPE) in susceptible snails, while in resistant snails elevated during the second WPE, and returned to initial level at 3 and 4 WPE. Susceptible snails had low detectable levels of TNF-α and INF- like-activity after infection. However, the resistant snails had significant low levels of TNF-α and INF- like-activity from 3 WPE until the 4th WPE without any sign of normalization. Histological sections in the head- foot region of susceptible and resistance B. truncatus infected with S. haematobium, mother sporocysts exists from1 to 7(day post exposure) DPE, in the susceptible snail the mother sporocysts were found as single, multiple<br />and mature types. No mother sporocysts were appear in the lip and mantle of the snail on 2, 5, 7 DPE and on 1-3 , 6 DPE respectively. In the resistant snails few mother sporocysts were found in the lip, mantle and tentacles. The results showed that schistosome-resistant Bulinus can be an alternative strategy for the control of schistosomiasis.
Bulinus truncatus,cytokine Schistosoma haematobium,Sporocyst,Susceptible,resistant
https://jesp.journals.ekb.eg/article_90379.html
https://jesp.journals.ekb.eg/article_90379_fec2aa5f5fd949ad557493ef7af31d95.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PHARMACOLOGICAL AND ANTIOXIDANT ACTIONS OF GARLIC AND/OR ONION IN NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD) IN RATS
295
308
EN
SAYED
H.
SEIF EL-DIN
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-
Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
ABDEL-NASSER
A.
SABRA
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-
Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
OLFAT
A.
HAMMAM
Department of Pathology, Theodor Bilharz Research Institute, Warak El-
Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
FATMA
A.
EBEID
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-
Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
NAGLAA
M.
EL-LAKKANY
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El-
Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
10.21608/jesp.2014.90380
Non-alcoholic fatty liver disease (NAFLD) includes a broad spectrum of fat-induced liver injury, ranging from mild steatosis to cirrhosis and liver failure. This study investigates the hepatoprotective properties of garlic and onion in NAFLD rat model. Ninety male Sprague- Dawley rats were randomly divided into 9 groups; normal (I), NAFLD induced with high fat diet (HFD; II), NAFLD switched to regular diet (RD; III), NAFLD-HFD or NAFLD-RD treated with garlic (IV, V), onion (VI, VII) or the combined garlic+onion (VIII, IX) respectively. A NAFLD rat model was established by feeding the animals with a high-fat diet for 12 wk. These animals were then treated with garlic or/and onion or vehicle for 8 wk (weeks 13-20) and then killed to obtain serum samples and liver tissues. Liver histology, lipids, parameters of oxidative stress, TNF- and TGF- were measured. The liver in NAFLD-HFD showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration. Serum levels of ALT, AST, ALP, leptin, cholesterol, triglycerides, TNF-α, TGF- and hepatic MDA were significantly increased (P<0.05) compared with normal group. This was accompanied with reduction of hepatic<br />GSH, GR, GPx, GST, SOD and serum adiponectin. These changes were to a less degree in NAFLD-RD group. Combined administration of garlic+onion produced a better and significant decrease in liver steatosis, serum liver enzymes, oxidative markers and lipid peroxidation versus each one alone. In the same time, NAFLD-induced inflammation was also mitigated via reduction of TNF- and TGF-. In addition, these results were better in the group IX versus group VIII.
Fatty Liver,Garlic,onion, leptin,adiponectin,TGF-,TNF-,liver enzymes and antioxidants
https://jesp.journals.ekb.eg/article_90380.html
https://jesp.journals.ekb.eg/article_90380_d197496dbf3f4724ff995da12844f10b.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
ANATOMICAL AND SURFACE MORPHOLOGY OF HEDRURIS LUTJANENSES SP. N. (NEMATODA: HEDRURIDAE) FROM THE COMMON MARINEWATER FISH LUTJANUS SYNAGRIS IN DAMIETTA, EGYPT
309
320
EN
M.
M.
RAMADAN,
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
E.
R.
AWAD
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
R.
G.
TAHA
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
10.21608/jesp.2014.90381
The description and tegumental ultrastructure of nematode Hedruris lutjanenses sp. n. collected from marine Lane Snapper fish Lutjanus synagris Linnaeus, 1758 collected at Manzala Lake, Damietta, Egypt was studied by light and Scanning Electron Microscopy (SEM). The new species is mainly characterized by that the buccal capsule is surrounded by four pseudolapia; one small and three larger pseudolapia, each one bears a number of papillae arranged irregularly on its surface and the presence of one pair of precloacal papillae and nine pairs of postcloacal ones in males.
Egypt,Damietta,Hedruris Lutjanenses,Sp. N. Lutjanus synagris
https://jesp.journals.ekb.eg/article_90381.html
https://jesp.journals.ekb.eg/article_90381_61a5e02155ab343e279207fdc3cc029b.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
MOLECULAR CLONING AND EXPRESSION OF THE LEISHMANIA TROPICA KMP-11 GENE
321
328
EN
MOUAYAD
MERIEE
Department of Animal Biology, Faculty of Sciences, Damascus University, Syria.
CHADI
SOUKKARIEH
Department of Animal Biology, Faculty of Sciences, Damascus University, Syria.
ABDUL QADER
A.
ABBADY
Department
of Molecular Biology and Biotechnology, Atomic Energy Commission of
Syria (AECS), Damascus, Syria.
10.21608/jesp.2014.90383
Kinetoplastid membrane protein-11 (KMP-11) is a small protein of 11 kDa present in all kinetoplastid protozoa studded so far. This protein which is highly expressed in all stages of the Leishmania life cycle is considered a potential candidate for a leishmaniasis vaccine against many leishmania species. KMP-11 has been recently described in Leishmania tropica.<br />In the present study, the KMP-11 gene was extracted from L. tropica by PCR using two oligonucleotide primers designed to amplify the entire coding region of this gene. Then, the purified PCR products were successfully ligated into a high expression vector the pRSETGFP. This expression vector provides the opportunity to clone the desired insert as a fusion<br />protein with a GFP and a tag, polyhistidine region. The GFP use as a carrier to improve immune response and the polyhistidine tag facilitates detection of the expressed protein with anti-His antibodies and also purification of the protein using affinity purification. After wards KMP-11 coding region was sequenced and the recombinant protein was induced and<br />purified from Escherichia coli cultures. The results of the present study will increase our knowledge about molecular cloning and expression of the L. tropica KMP-11 gene, and this may be used as an effective target for controlling cutenous leishmaniasis.
Leishmania tropica,Molecular cloning,expression
https://jesp.journals.ekb.eg/article_90383.html
https://jesp.journals.ekb.eg/article_90383_0ff495e321e9cc145fa75f1d8930a199.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
SCREENING OF TOXOPLASMA GONDII INFECTION AMONG CHILDBEARING AGE FEMALES AND ASSESSMENT OF NURSES’ ROLE IN PREVENTION AND CONTROL OF TOXOPLASMOSIS
329
342
EN
AHMED
MEGAHED AHMED
SALEH
Military Institute of Health and Epidemiology.+
HISHAM
ABD EL-RAOUF
ALI
The
Military Medical Academy, Egypt.
SALWA
ABDALLA MOHAMED
AHMED
Military Institute of Health and Epidemiology and Medical Surgical Nursing, The
Military Medical Academy, Egypt.
SAMAH
MOSTAFA
HOSNY
Military Institute of Health and Epidemiology, The
Military Medical Academy, Egypt.
TOSSON
A.
MORSY
Department of Parasitology, Faculty of Medicine,
Ain Shams University, Cairo 11566, Egypt.
10.21608/jesp.2014.90384
Toxoplasmosis, caused by Toxoplasma gondii is an obligate intracellular zoonotic protozoan parasite, with a worldwide distribution particularly in Arab countries including Egypt. The study evaluated toxoplasmosis infection among childbearing age Egyptian females and assessed the military nursing staff knowledge, attitude and compliance to toxoplasmosis prevention and control measures. The study was conducted in a general military hospital. Research design: cross-section descriptive research design was used to conduct this study. The subjects consisted of 14 young females (11 were in-patients undergoing gynecological treatment in a military hospital and 3 were staff nurses. On the other hand, 44 staff nurses were available for assessment who met the inclusion criteria Tools: 4 tools were used for data collection: first consisted of self-administered questionnaires to assess nurses’ socio-demographic data and knowledge, second rating scale to assess nurses’ attitude towards toxoplasmosis infection and its prevention, third performance check list to<br />measure nurses’ compliance to infection control measures, and fourth measured the anti- Toxoplasma antibodies by commercial indirect hemagglutination test (IHAT). The results showed that almost half of the nurses had satisfactory levels of knowledge, attitude, and compliance to toxoplasmosis infection control measures. 22.2% of the pregnant women<br />and 20% of non-pregnant ones showed antibodies against T. gondii. Thus health education about toxoplasmosis should be tailored to women whether married or single to help in avoiding the risk of infection. Frequent periodic IHAT should be done for people who continuously contact with cats. Adherence to strict infection prevention measures is a must to eliminate exposure to toxoplasmosis infection. Training intervention should be implemented to achieve successful improvement in knowledge, attitude, and compliance of toxoplasmosis control measures.
Toxoplasmosis,Knowledge,Attitude,Compliance,Nursing Staff,Military hospital
https://jesp.journals.ekb.eg/article_90384.html
https://jesp.journals.ekb.eg/article_90384_95ec7efbdb199423282dd12095539701.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
INITIAL EXPERIENCE OF SURGICAL MICROWAVE TISSUE PRECOAGULATION IN LIVER RESECTION FOR HEPATOCELLULAR CARCINOMA IN CIRRHOTIC LIVER
343
350
EN
A.
ABDELRAOUF
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical
Medicine Research Institute, Cairo, Egypt.
H.
HAMDY
Department of General Surgery,
Theodor Bilharz Research Institute, Giza 12411, Egypt.
A.
M.
EL ERIAN
National Institute of Endocrinology, National
Institute of Diabetes & Endocrinology, Cairo, Egypt.
M.
ELSEBAE
Department of General Surgery,
Theodor Bilharz Research Institute, Giza 12411, Egypt.
S.
TAHA
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical
Medicine Research Institute, Cairo, Egpt.
H.
E.
ELSHAFEY
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical
Medicine Research Institute, Cairo, Egypt.
S.
ISMAIL
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical
Medicine Research Institute, Cairo, Egypt.
M.
HASSANY
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical
Medicine Research Institute, Cairo, Egypt.
10.21608/jesp.2014.90385
Surgical hepatic resection has been considered as the first-line treatment which is most effective and radical treatment for HCC, however, HCC is usually associated with poor liver function owing to chronic hepatitis or liver cirrhosis. Techniques that can eradicate the tumor and also preserve liver function are needed. Moreover, hepatic resection, in the presence of cirrhosis, raises special problem of high risk as hemorrhage and liver failure, thus, good clinical results can only be achieved by minimizing operative blood loss, time of the intervention as well as the hepatic reserve. The tremendous progress in microwave technology has recently attracted considerable attention. This study evaluated the feasibility of this new liver transection technique demonstrating the high performance of this procedure, the accuracy in terms of squeeze effect on veins and portal branch and in terms of reducing the intra operative blood loss, and minimizing the operative time for safe hepatectomy. Twenty-six consecutive patients a first-time diagnosis of hepatocellular carcinoma (HCC) on<br />top of liver cirrhosis were recruited for the study, from August 2011 to January 2013.All patients were subjected to full clinical examination, laboratory investigations, abdomen ultrasound (U/S), triphasic computed tomographic liver scan (CT) and dynamic magnetic resonance imaging (MRI) in some doubtful cases. Inclusion requirements were presence of resectable disease without vascular invasion or extrahepatic spread at imaging, Child-Pugh class A & B (Score 7) liver cirrhosis, (INR) < 1.6or platelet count ›60 000/mm3 with no previous treatment. Patients were treated by applying pre-coagulation of the liver transection lines using microwave probe positioned in parallel to the line of resection by open approach after intraoperative U/S assessment for localization of the tumor and line of resection. The procedures were performed under general anesthesia. Mobilization of the liver was not necessary to be done in all cases. Intra-operative ultrasound (Aloka, Tokyo, Japan) was used to exclude lesions not detected pre-operatively (operative surprise), to define the location, number and size of the tumor and to identify large intra-hepatic large vascular and biliary structures,<br />and guide insertion of the microwave applicator. Intraoperative ultrasound was used again, to assess that the line of resection was done. Primary endpoints documented were total operative time, time for liver parenchyma transection, intraoperative blood loss and blood transfusion requirements. Secondary endpoints included postoperative complications, mortality and intensive care unit (ICU) together with hospital stay.
microwave,Hepatectomy - liver - Cirrhosis - Hepatocellular carcinoma
https://jesp.journals.ekb.eg/article_90385.html
https://jesp.journals.ekb.eg/article_90385_4013aef3d8a0241dabe8eb997f5c4c58.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
A DESCRIPTION OF HAEMOGREGARINA SPECIES NATURALLY INFECTING WHITE-SPOTTED GECKO (TARENTOLA ANNULARIS) IN QENA, EGYPT
351
359
EN
SOHEIR
A.H.
RABIE
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt.
ABDEL-NASSER
A.
HUSSEIN
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt.
10.21608/jesp.2014.90387
The present study describes the developmental stages of Haemogregarina species in the blood and tissues of naturally infecting white-spotted gecko Tarentola annularis collected from Qena, Egypt. Different parasite’s forms were observed infecting the erythrocytes. The gamonts enclosed within parasitophorous vacuole and seems to have no clear effect on the<br />host cell especially in case of immature parasite forms. But in the presence of mature gamonts the host cell nucleus displaced. The parasitaemia level is up to 280 per 10,000 erythrocytes counted. Trophozoites and gamonts have been recognized in the blood smears. The rounded trophozoite diameter is 3.84±0.87 μm, while the elongated trophozoite measured 4.42±0.69×2.8±0.56 μm. The mature gamonts were differentiated into two forms; short gamont measuring 10.82±0.82×3.30±0.73 μm (range: 10-12.1×2.2-4.4 μm) and the long gamont measured 14.67±0.83×3.96±0.77 μm (range: 14.1-16.5×3.3-5.5 μm). Merogony carried out only in the endothelial cells of the blood capillaries in the lung. Different merogonic stages have been recognized and differentiated in two forms; micromeront measured 13.250.50×120.0 μm and produces a few number of large merozoites, macromeront measured 19.750.87×13.250.50 μm and produces more small sized merozoites. The gamonts and merozoites have the general characteristic ultrastructures of the Apicomplexa containing components of the apical complex, e.g. pellicle, micronemes, rhoptries, and few dense bodies and subpellicular microtubules.
Haemogregarina,Tarentola annularis,Parasitaemia,light and electron microscope,Egypt
https://jesp.journals.ekb.eg/article_90387.html
https://jesp.journals.ekb.eg/article_90387_c9e85d0c5d6822dadf45de76d0eb8560.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
HYDROCARBON INSECTICIDES: THEIR RISKS FOR ENVIRONMENT AND HUMAN HEALTH
361
372
EN
MAMDOUH
M.
EL-BAHNASAWY
Military Medical Academy, Egypt.
AMINA
EL-HOSINI
MOHAMMAD
Military Medical Academy, Egypt.
TOSSON
A.
MORSY
Department of Parasitology, Faculty of Medicine, Ain
Shams University, Cairo 11566, Egypt.
10.21608/jesp.2014.90389
Insecticides are used to control diseases spread by arthropods, but theys vary greatly in toxicity. Toxicity depends on the chemical and physical properties of a substance, and may be defined as the quality of being poisonous or harmful to animals or plants. Poisons have many different modes of action, but in general cause biochemical changes which interfere<br />with normal body functions. Toxicity can be either acute or chronic. Acute toxicity is the ability of a substance to cause harmful effects which develop rapidly following absorption, i.e. a few hours or a day. Chronic toxicity is the ability of a substance to cause adverse health effects resulting from long-term exposure to a substance. There is a great range in the toxicity of insecticides to humans. The relative hazard of an insecticide is dependent upon the toxicity of the pesticide, the dose received and the length of time exposed. A hazard can be defined as a source of danger. The great majority of insecticides are poisonous to man and his beneficial insects and animals and are carcinogenic agents particularly, the halogenated hydrocarbons containing benzene ring.
Halogenated hydrocarbons,environmental pollution,Human health
https://jesp.journals.ekb.eg/article_90389.html
https://jesp.journals.ekb.eg/article_90389_8d95ab6b521408de6189921ae5a3b2ab.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
DESCRIPTION OF TWO NEW CERCARIAE (AN ECHINOSTOME CERCARIA AND A XIPHIDIOCERCARIA) PROCURED FROM BIOMPHALARIA PFEIFFERI (KRAUSS) FROM NIGERIA
373
380
EN
FOUAD
YOUSIF
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, Imbaba, Egypt.
MAGDA
AYOUB
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, Imbaba, Egypt.
MENERVA
TADROS
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, Imbaba, Egypt.
SAMIA
EL BARDICY
Department of Environmental Research and Medical Malacology, Theodor Bilharz
Research Institute, Imbaba, Egypt.
SIMON
ABOLARINWA
Department of Biological Sciences, Federal
University of Technology. Minna, Niger State, Nigeria.
10.21608/jesp.2014.90391
During parasitological examination of Biomphalaria pfeifferi snails obtained from Niger state (Nigeria), 2 new types of cercariae were found. They are identified to the level of referring to the major group and described here for the first time. They were examined viable and stained with vital stains as well as fixed in 70% alcohol. They were drawn with a camera lucida and photographed. They are identified as an echinostome cercaria and a xiphidiocercaria. The echinostome is characterized by having a ventral sucker almost double in size the oral one. It has a semicircular structure located beyond the oral sucker. Three pairs of penetration glands are found at the anterior portion of the body. The number of collar spines is relatively large (44-46). The flame cells are 17x2 in number. Two main lateral excretory ducts extend anteriorly,<br />form two typical echinostome loops then pass posteriorly to open together in a diverticulated excretory vesicle. Its tail is relatively long and flattened with 3 fin folds. The tail (640 μm) is longer than the body (475μm). The xiphidiocercaria belongs to the "ornatae" group. It is relatively small (180.5x110μm) with a long stylet (30μm). Its oral sucker is one and half times the size of the ventral sucker. Two excretory ducts extend posteriorly in both sides and become dilated and unite to open in a circular excretory vesicle. Tail is slender shorter than the body and has a dorso-ventral fin fold.
Echinostome cercaria,xixphidiocercaria,Biomphalaria pfeifferi,Nigeria
https://jesp.journals.ekb.eg/article_90391.html
https://jesp.journals.ekb.eg/article_90391_d0bcbd30d2cc2e3e3af1f73848a78ea0.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
BRONCHIAL ASPIRATES GLUCOSE LEVEL AS INDICATOR FOR METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN INTUBATED MECHANICALLY VENTILATED PATIENTS
381
388
EN
SHERIF
ALSAYED
Department of Chest, Faculty of Medicine, El Fayoum University, Egypt.
SAMAR
MARZOUK
Department of
Biochemistry, Faculty of Medicine, Cairo University, Egypt.
ESSAM
MOUSA
Department of Internal Medicine,
Faculty of Medicine, Al Azhar University, Egypt.
ASHRAF
RAGAB
Department of Anesthesia,
Faculty of Medicine, Cairo University, Egypt.
10.21608/jesp.2014.90392
This study evaluated if the level of glucose in bronchial aspirate serves as indicator for the risk of MRSA infection in intubated mechanically ventilated ICU patients. A total of 50 critically ill patients was enrolled and were under tight glycemic control to abolish the effect of hyperglycemia on bronchial secretion, if they were expected to require mechanical<br />ventilation for more than 48 hours. Bronchial aspirates were detected for glucose and sent twice weekly for microbiological analysis and whenever an MRSA was expected. The results showed that all the patients had glucose tested in bronchial aspirates. Glucose was detected in bronchial aspirates of 28 of the 50 patients. Glucose in bronchial aspirates in these<br />patients ranged between (2.9-5.1mmol/l). MRSA was detected in 22 patients where 28 were MRSA free of the MRSA patients 19 had positive glucose where glucose was positive in 28 patients of them 19 (86.4%)where MRSA positive to 9 with no MRSA (32.1%).The risk of having MRSA present markedly increased significantly in the presence of glucose: (p value .001)
Glucose level,Indicator,Methicillin-resistant Staphylococcus aureus,Ventilated patients
https://jesp.journals.ekb.eg/article_90392.html
https://jesp.journals.ekb.eg/article_90392_478f4d043eb9cda0fc2f9e14ab2a3ef3.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PRELIMINARY STUDY ON CRYPTOSPORIDIOSIS IN LIVESTOCK FROM KUWAIT
389
392
EN
QAIS
A. H.
MAJEED
College of Basic Education, The Public authority for Applied Education and Training,
Kuwait.
MAHA
S. H.
ALAZEMI
Department of Criminal Evidences, Ministry of Interior, Kuwait.
10.21608/jesp.2014.90393
Out of 225 fecal samples (40 of calves, 128 of lambs and 57 of goat kids) received in the Veterinary Laboratories, Kuwait, 43 (19.1%) were found to be positive for Cryptosporidium using the conventional method, modified acid-fast stain, and the immunologic technique, the immuno-chromatography assay. Calf fecal samples showed the highest infection rate (37.5%),<br />followed by goat kids (21%) and then Lambs (12.5%). Results obtained by conventional method were found to be consistent with those detected using the immunologic technique.
cryptosporidiosis,livestock,Diagnosis microscopic,Immunology
https://jesp.journals.ekb.eg/article_90393.html
https://jesp.journals.ekb.eg/article_90393_24bc860f84bf136d9939a9f69c46b9cc.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PREVALENCE OF ANTI-TOXOPLASMA GONDII ANTIBODIES IN ABORTED EWES IN KUWAIT
393
396
EN
MAHA
S. H.
ALAZEMI
Department of Criminal Evidences, Ministry of Interior, Kuwait.
10.21608/jesp.2014.90394
Serum samples from 528 aborted ewes from different sheep flocks in Kuwait were tested for Toxoplasma gondii. The indirect hemagglutination test (IHAT) was used with a titer of 1:80 considered positive. Antibodies to T. gondii were found in 94 (17.8%). The highest number of positive ewes (23; 24.5%) had a titer of 1:640. Only one aborted ewe had a titer of<br />1:2560. This study is the first to report Toxoplasma infection in sheep which may pose risk to humans who consume under cooked mutton. The detection of Toxoplasma antibodies in aborted ewes refers to the implication of toxoplasmosis in abortion among sheep in Kuwait.
Kuwait,Toxoplasmosis,sheep,Abortion,IHAT
https://jesp.journals.ekb.eg/article_90394.html
https://jesp.journals.ekb.eg/article_90394_cbc12dcf39f983a081dc9ba8bb17af48.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PREVALENCE OF SCHISTOSOMIASIS IN AL-BAHA PROVINCE, SAUDI ARABIA IN YEARS 2012 AND 2013 (PROSPECTIVE AND COMPARATIVE STUDY)
397
404
EN
KHAIRY
ABDELHAMID
MOHAMMAD
Departments of Parasitology and Faculties of Medicine, Al-Azhar University, Cairo,
Egypt and Al-Baha University, Al-Baha, Saudi Arabia.
10.21608/jesp.2014.90395
The rate of human schistosomiasis increased in Al- Baha Province, Saudi Arabia in the last few years. So, it was necessary to study the prevalence of human schistosomiasis in Al- Baha Province over the period of the two years (2012-2013).<br />In general, there was a decline in the prevalence of schistosomiasis in Al- Baha over the study period and it was significantly less in 2013 than it was in 2012. Schistosomiasis infection rate was affected by host sex as males had higher infection rate than females. Age group of 15-44 showed the highest infection rate. Prevalence of schistosomiasis in Saudi<br />people was significantly higher than it was in non-Saudi or immigrant ones. Thus, more attention should be paid to Al-Baha area in the future schistosome control programmes.
Saudi Arabia,Al-Baha,Schistosomiasis,Prospective study
https://jesp.journals.ekb.eg/article_90395.html
https://jesp.journals.ekb.eg/article_90395_79a28fd05a5981e3fb206a5414b13b4f.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
AN INTERVENTIONAL PROGRAM FOR NURSING STAFF ON SELECTED MASS GATHERING INFECTIOUS DISEASES AT HAJJ
405
424
EN
MAMDOUH
M.
EL-BAHNASAWY
Consultant of Endemic Diseases and Fevers, Military Medical Academy, Egypt.
NAGWA
ZEIN EL ABDEEN A.
ELMENIAWY
Military
Medical Institute, Maadi Military Hospital, Egypt.
TOSSON
A.
MORSY
Department of Parasitology,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
10.21608/jesp.2014.90396
This work improved military nursing staff knowledge on selected mass gathering infectious diseases at Hajj. The results showed that only (20%) of the participating nurses attended training program about health hazard during pilgrim. But only (40.0%) of them found the training programs were specific to nurses. Majority found the program useful (70.0%), and the average duration of this training program in weeks was 3.5+1.1. There was significant improvement P=<0.001, of correct knowledge about meningitis regarding causes, organisms, mode of spread, people at risk, transmission,<br />prevention and treatment, the highest improvement was in causes of meningitis the lowest was in adult vaccination. 25% of participants had adequate knowledge (>60% from total score) in pre-test 93% in post-test 72% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement of correct knowledge P=<0.001 about seasonal influenza and respiratory diseases during pilgrim, the highest improvement was in influenza vaccine strains the lowest was in antiviral drugs. 23% of nurses had adequate knowledge (>60% from total score) in pre-test 94% in post-test 66% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement P=<0.001 of correct knowledge about gastrointestinal diseases and food poisoning during pilgrim among nurses at military hospital, the highest improvement was in risk factors of food poisoning the lowest was in what GE patient should do. 22% of participants had adequate knowledge (>60% from total score) in pretest 91% in post-test 58% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement P=<0.001 of correct knowledge about heat exhaustion during pilgrim among nurses at military hospital, the highest improvement was in non-communicable diseases the lowest was in sun stroke prevention. 27% of participant had<br />adequate knowledge (>60% from total score) in the pre-test 94% in the post-test 74% after 3 month with significant difference among pre, post and FU regarding adequate knowledge. Also, there were significant improvement P=<0.001 of correct knowledge about hypertension, dengue fever, skin scalding & others diseases during pilgrim among nurses at military hospital, the highest improvement was in skin scalding prevention the lowest was in first aid bag. 28% of participant had adequate knowledge (>60% from total score) in the pre-test 92% in the post-test 61% after 3 month with significant difference among pre, post and FU regarding adequate knowledge. There was a significant difference between total knowledge score according to education, and work experience (P>0.05) in the pre, post and after 3 month in age and in all intervention time in department the highest was ICU then ward then operation room.
Egypt,Military Nursing Staff,Mass gathering infectious diseases,pilgrimage
https://jesp.journals.ekb.eg/article_90396.html
https://jesp.journals.ekb.eg/article_90396_48fd1d0b655ce8f94ceb7e3301688b84.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
NATURAL AND EXPERIMENTAL EVIDENCE OF VISCEROTROPIC INFECTION CAUSED BY LEISHMANIA TROPICA FROM NORTH SINAI, EGYPT
425
434
EN
SAID
A.
DOHA
Research and Training Center on vectors of diseases, Faculty of Science building,
Ain Shams University, Egypt.
MAGDI
G.
SHEHATA
Entomology Department, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.
ADEL
R.
FAHMY
Entomology Department, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.
ABDALLAH
M.
SAMY
Entomology Department, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.+
10.21608/jesp.2014.90398
Cutaneous leishmaniasis (CL) is a neglected clinical form that is quite prevalent in Eastern North parts of the country in Sinai Peninsula. Leishmania tropica was identified by previous reports as the causative agent responsible for viscerotropic infections in patients and experimental animals. Here, we reported the viscerotropic infections from naturally infected rodent Gerbillus pyramidum floweri collected from North-Sinai. Footpad and tail lesions, spleenomegaly, and malformed dark-colored spleen were the characteristic CL symptoms. The spleen of the rodent found positive to amastigote impression smear. ITS-1 DNA was sequenced and revealed 100% identity of the strain in the current study to the other L. tropica sequences identified from the patients with the suspected CL and inhabited the same study area. The current findings confirmed the susceptibility of gerbil to L. tropica, and raise the concerns for the role of rodents as accidental host suffering the infections. The susceptibility of wild and experimental rodents to the same L. tropica strain was also investigated; BALB/c and G. pyramidum were more susceptible to L. tropica (24.33±4.37 and 25±4.58 days post-infection, respectively). Similar viscerotropic pathologies were reported in experimental infection of only golden hamster (≈ 120 days post-infection), and G. p. floweri (≈ 160 days post-infection).
Viscerotropic,L. tropica,North Sinai,ITS-1 DNA,Splenomegaly
https://jesp.journals.ekb.eg/article_90398.html
https://jesp.journals.ekb.eg/article_90398_3d6861d3eec113291459811d632d2ff4.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PFEIFFERINELLA SP. (PFEIFFERINELLIDAE, APICOMPLEXA) INFECTING THE FRESH WATER SNAIL PIRENELLA CONICA LIGHT AND ELECTRON MICROSCOPE STUDIES
435
446
EN
HODA
M.
EL-FAYOMI
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
HAYAM
MOHAMMED
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
THABET
SAKRAN
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
10.21608/jesp.2014.90399
Coccidian oocysts were proved to be found in 70 of 100 collected Pirenella conica snails, with a natural infection of 70%. It was observed that, Pfeifferinella sp. was transferred between hepatopancreas and small intestine of snail. The prepatent period of Pfeifferinella sp. infecting P. conica snails ranged from 14-18 days and the patent period was reached 50 days (P.I.). Merogony stages were the early stages observed in this study. These stages were observed in the hepatopancreas and in a large clear parasiteophorous vacuole (PV). In snails killed 4 days P.I. immature meronts were measured 12 х 10 μm containing 8 nuclei. Meanwhile, mature meronts with about 6 differentiated merozoites were detected as early as 6 days P.I., and measured 3.1х1.4μm. The earliest gametogonic stages were seen in the intestine of Pirenella conica snails<br />killed 12 days P.I. Microgamonts contained about 4 nuclei and measured 7.9х6.7μm. The macrogamonts measured 7.3х5.6μm. Macrogametes were characterized by the presence of the vaginal tube, this tube measured 4.3х1.1μm. Fertilization was occurred in the intestine of the infected snails at 12 days P.I. Zygotes developed into young oocysts after fertilization. Sporogony occurred in the intestine. In the earliest stage, the nucleus of young oocyst was occupied the central position that were observed through the examination of the intestine of infected Pirenella snails at 14 day P.I. These oocysts were found to be colorless and ellipsoid or spherical in shape measured 9.5 х 8.5μm. The oocyst wall consists of two layers, micropyle and micropyle cap were not observed in these oocysts, and wall forming bodies were arranged at the periphery of oocyst directly under the developed oocyst wall. Sporulated oocyst contains 8 sporozoites<br />filling the entire cavity of the oocyst without sporocyst formation. Fully sporulated oocysts were excreted in the faces of infected snails from 14-18 day P.I., these oocysts measured 9.5х8.5μm. Micropyle was absent and while a residual body was observed.
Egypt,Beni Suef Governorate,Pfeifferinella sp,Pirenella conica,Morphology
https://jesp.journals.ekb.eg/article_90399.html
https://jesp.journals.ekb.eg/article_90399_0ca8f97ddb427fb341fe30c41683af52.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
THE EFFECT OF LARVAL AND ADULT NUTRITION ON SURVIVAL AND FECUNDITY OF DENGUE VECTOR AEDES ALBOPICTUS SKUS (DIPTERA: CULICIDAE)
447
454
EN
ABEER
S.
YAMANY
Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt.
FATMA
K.
ADHAM
Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt.
10.21608/jesp.2014.90400
The effect of larval and adult nutrition on survival and fecundity of the dengue vector Aedes albopictus Skuse (Diptera: Culicidae) were studied under laboratory conditions, the energy for the physiological activities for both male and female mosquitoes is provided largely by their eserves during larval stage and affected by adult diets. Two groups of larvae (A, B) were reared at 27±3oC, 70-80% R.H. and DL. 12: 12. Group (A) with 200 larvae (high larval diet) and Group (B) with 600 larvae (low larval diet). Ae. albopictus exhibited increased fecundity and egg hatch success. Immature development was quick. Immature survival was high, with lowest rate in the pupal stage. Highest longevity was observed in large females fed water + 10% sucrose solution (29.571±0.415 days) while the lowest one was (1.3±0.132 days) in starved small females. Large females have significantly (P< 0.001) higher fecundity than smaller females, regardless of whether the females were provided 10% sucrose solution or not (524±0.203 eggs/group 159.714±0.1997 eggs/group), respectively. The addition of 10% sucrose solution significantly (P< 0.001) increase the fecundity regardless of whether large or<br />small females (657.9±0.2198 eggs/group, 242.429±0.119 eggs/group), respectively.
nutrition,longevity,fecundity,Aedes albopictus
https://jesp.journals.ekb.eg/article_90400.html
https://jesp.journals.ekb.eg/article_90400_4559c4edaaaceddb36554478f5faed88.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
UPDATE IN DIAGNOSIS AND TREATMENT OF CRYPTOSPORIDIOSIS AN UPDATE REVIEW
455
466
EN
OSAMA
H.
ABDALLA
Department of Medical Parasitology, Faculty of Medicine, South Valley
University, Qena, Egypt.
10.21608/jesp.2014.90402
Cryptosporidiosis represents a major public health problem which transmitted by contamination of food or water by sporulated Cryptosporidial oocyst. Causing self- limited diarrhea in immunocompetent person and chronic and life threatening diarrhea among immunocompromised individuals. It can be diagnosed by concentration and detection of its Oocyst in different environmental samples and water by microscopic and immunological examination such as enzyme immunoassay (ELISA) for parasite antigens and nucleic acid amplification assay as well as use of molecular techniques such as Polymerase Chain Reaction (PCR). Treatment is face challenges, Macrolides, Paramomycin, Nitazoxanide and Mirazid. All these drugs have partial efficacy in reducing disease severity in immunocompetent individuals. Nitazoxanide has partial efficacy in immunocompromised individuals. Resolution of Cryptosporidiosis can be maintained with effective Highly Active Antiretroviral Therapy (HAART).
Cryptosporidosis,C. parvum,C. hominis,diarrhea,Diagnosis,Treatment
https://jesp.journals.ekb.eg/article_90402.html
https://jesp.journals.ekb.eg/article_90402_a23e4e92b329d64010e03fb830dd2be0.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
UROTHELIAL CARCINOMA OF THE URINARY BLADDER MIXED WITH SQUAMOUS DIFFERENTIATION OR SQUAMOUS CELL CARCINOMA IN AREAS WITH SCHISTOSOMIASIS IS SHOWING HIGH RISK OF RECURRENCE AND POOR SURVIVAL
467
473
EN
MOHAMED
WISHAHI
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba,
Giza, POB 30, Egypt.
HOSSAM
ELGANZOURY
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba,
Giza, POB 30, Egypt.
AMR
ELKHOULY
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba,
Giza, POB 30, Egypt.
MOHAMED
BADAWI
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba,
Giza, POB 30, Egypt.
10.21608/jesp.2014.90403
In schistosomiasis haematobium areas endemic, bladder cancer is the first cause of malignancy in men and fourth in women. The chronic schistosomiasis would lead to variant histologic patterns which manifest in squamous cell carcinoma (SCC) or squamous differentiation (SqD). This study evaluated the clinical outcome after radical cystectomy (RC) in patients with urothelial carcinoma (UC) mixed with SCC or SqD, Comparison was done with two arms of pure UC and pure SCC, indication for RC was muscle-invasive-disease, and evaluation included recurrence, metastases, and overall survival. The data of patients treated with RC for muscleinvasive-disease, selection was revised for 127 patients with urothelial carcinoma mixed with SCC/SqD, two comparative arms were 100 patients with pure UC, and 100 patients had pure<br />SCC. Follow up was on 8 months, 3years, and 5 years to detect recurrence, metastasis, and overall survival in the three groups<br />The results showed that by comparison of disease aggressiveness in the three groups regarding<br />recurrence, metastasis, and overall survival was analysed. Overall survival with mixed tumours<br />was significantly lower than pure UC or SCC, recurrence and metastases were higher in mixed<br />tumour which was an independent factor for poor prognosis and low survival.
Egypt,Schistosomiasis,Bladder cancer,Urothelial carcinoma,Squamous cell carcinoma,differentiation,Mixed tumour,prognosis
https://jesp.journals.ekb.eg/article_90403.html
https://jesp.journals.ekb.eg/article_90403_ac3dee4b03ccd4b4939f93a11a092266.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
EFFICACY OF PENTOXIFYLLINE AS AN ANTIFIBROTIC DRUG IN EXPERIMENTAL MURINE SCHISTOSOMAL HEPATIC FIBROSIS
475
488
EN
EMAN
A.
KHALIFA
Department of Microbiology, Faculty of Medicine, Taif University, Taif,
Kingdom of Saudi Arabia.
DALAL
M.
NEMENQANI
Department of Pathology, Faculty of Medicine, Taif University, Taif,
Kingdom of Saudi Arabia.
10.21608/jesp.2014.90404
This study evaluated the possible antifibrotic effect of pentoxifylline on experimentally induced schistosomal hepatic fibrosis and its effect on serum leptin and transforming growth factor- β1 levels as possible antifibrotic mechanisms in correlation with the hepatic fibrosis indices. A total of ninety clean laboratories bred, males Swiss, albino mice were included, of which ten mice served as a control non-infected, non-treated group and sacrificed at one time. Eighty mice, each was subcutaneously infected with 50 Schistosoma mansoni cercariae and classified into groups: GI (infected & non-treated), GII (infected & treated with Mirazid®), GIII (infected & treated with Pentoxifylline®) and GIV (infected & treated with a combination of Mirazid and Pentoxifylline). Each group was further subdivided into 2 subgroups; subgroup ‘a’ which started treatment at 6th week post-infection (P.I.) and sacrificed at the end of 9th week P.I and subgroup ‘b’ which started treatment at 14th week P.I and sacrificed at the end of 17th week P.I. The efficacy of the treatment was assessed by histopathological examination of the liver with measurement of granuloma sizes, estimation of hydroxyproline content in the liver, and assessment of serum levels of leptin and transforming growth factor- ß1 (TGF- ß1) Mirazid (MZD) caused significant reductions in granuloma sizes and hepatic hydroxyproline content and caused non-significant reductions in serum levels of leptin and transforming growth factor- ß1 at 9th & 17th weeks P.I (GII). Pentoxifylline (PTX) caused significant reductions in granuloma sizes, hepatic hydroxyproline, and serum levels of leptin and transforming growth<br />factor- ß1 at the 9th & 17th weeks P.I (GIII). While combined therapy of both MZD & PTX in GIV caused more reductions in granuloma sizes, hepatic hydroxyproline, and serum levels of leptin and TGF- ß1 at the 9th & 17th weeks P.I when compared to the other groups.
Mice,Schistosomal hepatic fibrosis,Mirazid,Pentoxifylline,Histo-pathology
https://jesp.journals.ekb.eg/article_90404.html
https://jesp.journals.ekb.eg/article_90404_cb1a726936530f880eddd94ab96ba103.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PREVALENCE OF SCHISTOSOMIASIS AMONG PATIENTS ATTENDING QENA GENERAL HOSPITAL OVER THE YEARS 2011 AND 2012
489
495
EN
FATMA
GALAL
SAYED
Departments of Parasitology, Faculty of Medicine,
Assuit University, Egypt.
MOHAMMED
ALSALAHY
MOHAMMED
Departments of Parasitology, Faculty of Medicine,
Assuit University, Egypt.
MOHAMMED
SAYED
ABD-EL-KADER
Departments of Parasitology, Faculty of Medicine,
Assuit University, Egypt.
OSAMA
HUSSEIN
ABDALLA
Departments of Parasitology, Faculty of Medicine,
Assuit University, Egypt.
SAMAR
S.
AHMED
Community Medicine, Faculty of Medicine, South Valley University, Egypt.
10.21608/jesp.2014.90406
Schistosomiasis is a public health problem in Egypt. To detect the prevalence of schistosomiasis in Qena Governorate, 1601 urine and stool samples of patients attending Qena General Hospital were examined of whom 1601 patients had urinary symptoms and 893 patients had dysentery and bloody stool. Sheets were filled out on each patient. Also, a total of 7590 Bulinus truncatus and Biomphalaria alexandrina snails were collected from water bodies in Qena Governorate. The<br />overall prevalence of S. haematobium was 13.9%; with maximum among 6-18 year-old age group and higher in males than in females. Risk factors for S. haematobium infection were this age group; particularly males. However, males swim and play in water bodies and women wash cloths and utensils there, and children swimming or playing in canals. The S. haematobium peak of infection was in winter correlated with the highest prevalence in Dandara City, Qena City, Awlad-<br />Amr and El Hijarat. S. mansoni were negative in the examined individuals. The infective rate among snails was 1.82% in B. truncatus and 0.0% among B. alexandrina and M. tuberculata.
Qena Governorate,Schistosomiasis,Snails intermediate hosts,Parasitologic examination
https://jesp.journals.ekb.eg/article_90406.html
https://jesp.journals.ekb.eg/article_90406_798b719d57173f7d76b1e4a007a13d9e.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PATIENT SAFETY ATTITUDE AMONG HEALTH CARE PROVIDERS IN FAMILY HEALTH CARE CENTERS IN CAIRO GOVERNORATE
497
508
EN
MOHSEN
ABDALHAMED
GADALLAH
Department of Community, Environmental and Occupational Medicine, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
HASNAA
ABDALAAL
ABOUSEIF
Department of Community, Environmental and Occupational Medicine, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
DINA
NABIH KAMEL
BOULOS
Department of Community, Environmental and Occupational Medicine, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
HANAA
HASSAN ABDALLAH
ELHARONI
Specialist in Family Medicine,
Ministry of Health and Population, Cairo, Egypt.
10.21608/jesp.2014.90408
Two hundred and eighty Health care; physicians, nurses and technicians were included in the study, 133 (47.5) from 6th discrete HCF, 54 (19.3%) from Duwaiqa and 93 (33.2%) from Al Haggana. Ages ranged between 22 and 59 years (35.14±10.13), years of experience in the study group ranged between 1 and 35 years (8.72±8.18) and years of work in group ranged between 1 & 40 years (10.43±8.33). Doctors and nurses were males (72.55%) & females (86%) respectively while technicians were mostly males (60%). The teamwork climate score was 3.98±0.87, 64.0% answered high or very high, 16.4% answered inadequate and 18% had answered few or very few. Safety climate score was 3.61±0.63, 49.28% had high or very high score, 17.14% answered inadequate and 32.15% had answered few or very few. Job satisfaction score was 3.91±0.80, 32.15% had answered few or very few, 17.14% answered inadequate and<br />49.28% answered high or very high. Stress recognition score was 3.61±0.79, 25% had answered few or very few, 28.6% answered inadequate and 45.7% answered high or very high. Perception of management score was 3.48±0.80, 23.2% had answered few or very few, 17.8% answered inadequate and 57.6% answered high or very high. Working condition score was 3.51±0.84, 46.8% had answered few or very few, 17.1% answered inadequate and 35.7% answered high or very high. A significant difference regarding team work score, safety climate score, perception of management score, working condition score with highest value in doctors and lowest in technicians. On the other hand no significant difference was detected regarding job satisfaction score and stress recognition score. A significant difference regarding team work score, safety climate score and perception of management score with high values among older groups. No significant difference was detected regarding job satisfaction score, stress recognition score and working condition score. Also, a<br />significant difference regarding team work score and safety climate score with higher value in males. Significant difference regarding job satisfaction score and working condition score was detected with higher value in females. No significant difference was detected regarding stress recognition score and perception of management scores.
Egypt,Family Health Care Centers. Patient Safety,Health Care Providers
https://jesp.journals.ekb.eg/article_90408.html
https://jesp.journals.ekb.eg/article_90408_47adaabb90c9a9d35054905c995e2a8c.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
COMPARISON BETWEEN ELISA AND VARIOUS STAINS TECHNIQUES IN LABORATORY DIAGNOSIS OF CRYPTOSPORIDIOSIS
509
516
EN
NABIL
S.
GABR
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519,
Egypt.
MANAL
Z. M.
ABDELLATIF
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519,
Egypt.
EKHLAS
H.
ABD EL-HAFEEZ
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519,
Egypt.
REHAM
A. M.
ABD RABOU
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519,
Egypt.
10.21608/jesp.2014.90410
Cryptosporidium spp. is an important parasitic protozoa causing diarrhea which is a severe life-threatening diarrhea especially in immunocompromised hosts. We aimed to evaluate the usefulness of detection of Cryptosporidium spp. copro-antigen from fecal specimens by using enzyme linked immunosorbent assay (ELISA) test and comparing its sensitivity and specificity with some staining methods. The results revealed that Modified Acid-Fast stain is considered better than Giemsa in detecting Cryptosporidium species oocysts in faecal smears as their sensitivity were 67.5% and 53.75% respectively. On contrary, ELISA technique is considered the best method used for detection of cryptosporidial infection as its sensitivity is 90%.
Egypt,Cryptosporidium parvum,persistent diarrhea and ELISA technique
https://jesp.journals.ekb.eg/article_90410.html
https://jesp.journals.ekb.eg/article_90410_7d98c9c3481ca303b6573d849dbc7ea5.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PREVALENCE OF INTESTINAL PARASITES AND ITS IMPACT ON NUTRITIONAL STATUS AMONG PRESCHOOL CHILDREN LIVING IN DAMANHUR CITY, EL-BEHERA GOVERNORATE, EGYPT
517
524
EN
ADHAM
MOHAMMAD
HEGAZY
Departments of Pediatric, Faculty of Medicine, Ain Shams
University, Cairo 11566, Egypt.
NEVEEN
TAWAKOL
YOUNIS
Departments of Pediatric, Faculty of Medicine, Ain Shams
University, Cairo 11566, Egypt.
HEBA
ABDELKADER
AMINOU
Department of Parasitology, Faculty of Medicine, Ain Shams
University, Cairo 11566, Egypt.
AYMAN
MOHAMED
BADR
Ministry of Health and Population, Cairo, Egypt.
10.21608/jesp.2014.90412
This cross sectional study was done in Damanhur City, the Capital of El-Behera Governorate to detect the prevalence of intestinal parasitic infection among preschool aged children and to find out its effect on their nutritional status. Five-hundreds children aged between 2-6 years were enrolled; a detailed questionnaire, complete clinical and anthropometric assessment as well as complete stool analysis and blood picture were done. The study revealed that 51.8% preschool children were infected; Cysts of Entamoeba histolytica and Giardia lamblia were found in 16.8%, and 14.8% respectively. In 1.8% of cases, both cysts were found together. Ova of Ascaris lumbricoides, Enterobius vermicularis, Ancylostoma duodenale and Hymenolepis nana were detected in 14%, 3.4%, 5% & 0.2% of cases, respectively. In 0.4% of cases, ova of A. lumbricoides and E. vermicularis were found together. Mixed infections were found in 3.6% of children. Significantly lower weight for age z-score (WAZ) and weight for height z-score (WHZ) were found among infested children compared to non-infested ones (P<0.05). Moreover, stunting was found in 44.1%, underweight in 39.1% and wasting in 11.5% of infested children. Prevalence of anemia among all studied population was 39%; 48.6% in infested group compared to 28.8% in non-infested children (x=20, P<0.001). Improper hand washing, and playing in the street bare footed, together with playing with animals and family history of parasitic infestation were considered the independent predictors of parasitic infestation by using binary logistic regression.
Egypt,Preschool-children,Intestinal parasites,nutrition,anthropometric measurements
https://jesp.journals.ekb.eg/article_90412.html
https://jesp.journals.ekb.eg/article_90412_3d01d5849a57a41ebe324d44fb3df7e1.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
44
2
2014
08
01
PREDICTIVE VALUE OF PHOSPHORYLATED INSULIN–LIKE GROWTH FACTOR BINDING PROTEIN-1(PIGFBP-1) (BEDSIDE TEST) IN PRETERM LABOR
525
530
EN
AMRO
ELSAYED
ABO EL-EZZ
Department of Obstetrics and Gynecology Faculty of Medicine, AL-Azhar University,
Nasr City, Egypt.
ALSAEED
ELSAYED A.
ASKAR
Department of Obstetrics and Gynecology Faculty of Medicine, AL-Azhar University,
Nasr City, Egypt.
10.21608/jesp.2014.90413
This study evaluated the predictive value of phosphorylated insulin like growth factor binding protein-1 in cervical secretion as bedside test for prediction of preterm labor in symptomatic women. A total of 57 patients with singleton pregnancy at 24-34 weeks gestation, with symptoms suggestive of preterm labor were included in this study. A rapid cervical sample for PIGFBP-1 determination (Actim partus test, Medix Biochemical, and Kaunianen, Finland) was taken by<br />means of a polyester-tipped swab during a speculum examination of the cervix, and extracted with specimen- extraction solution. We analyzed the prevalence of preterm labor in these patients within seven days upon admission. And calculate sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for PIGFBP-1. This study was done at Kuwait Oil Company hospital (KOC) and Taiba hospital (Kuwait) during the period between April 2011 and June 2012.<br />The test was positive in 50.9% of patients and negative in 49.1%, among those tests was positive<br />64% delivered less than a week, and among those tests was negative 35.7 delivered less<br />than one week, with 74.3% sensitivity, 61% spesivity, 76.3% PPV and 73.6% NPV.
phosphorylated insulin –like growth factor binding protein,bedside test,preterm labor
https://jesp.journals.ekb.eg/article_90413.html
https://jesp.journals.ekb.eg/article_90413_1914e037458fb0982e4588edb9d1e380.pdf