The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
SEROTYPES OF DENGUE VIRUSES CIRCULATING IN JAZAN REGION, SAUDI ARABIA
235
246
EN
ADEL
A.
ALSHEIKH
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
O.
M.
DAFFALLA
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
E.
M.
NOURELDIN
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
W.
S.
MOHAMMED
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
K.
J.
SHRWANI
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
Y.
A.
HOBANI
National Center for Vector-Borne Diseases, MoH, Jazan, Saudi Arabia.
A.
D.
ABKAR
Department of Medical Parasitology, Saudi Arabia.
F.
A.
ALSHEIKH
Faculty of Medical Laboratory Sciences, University of Gezira, Sudan,
Faculty of Public Health, Jazan University, Saudi Arabia.
A.
M.
ASSIRI
Minister Deputy Assistant for Preventive
Health, MoH-Riyadh, Saudi Arabia.
10.21608/jesp.2017.77745
Dengue fever is considered to be the most important mosquito-borne disease and considered as endemic disease in Jazan region, Saudi Arabia. The present study aimed to analyze the prevailing dengue virus serotypes in the region. Serum samples of 220 suspected dengue cases were collected throughout 2016 and tested by one step Reverse Transcription Polymerase Chain Reaction (RT-PCR) with a set of specific primers for detection of four DV serotypes followed by sequencing the PCR products to confirm the results. Out of the 220 serum samples, 124 were found positive for dengue infection (56.4%). Three dengue virus serotypes were detected; DEN- 1, DEN-2 and DEN-3. DEN-2 is the most common and predominant type in the region rating 83.9% (104/124), followed by DEN-1 8.9% (11/124), and then DEN-3 7.2% (9/124). The high seroprevalence of dengue virus infections in Jazan region indicates its endemicity. The present study highlights the importance of tracking the spread of dengue virus types and its implication for analyzing changes in dengue endemicity in specified areas over time.
Dengue fever,Serotypes,1,2 & 3,Jazan region,Saudi Arabia
https://jesp.journals.ekb.eg/article_77745.html
https://jesp.journals.ekb.eg/article_77745_bf35cc57c1ad830e18b5aa2fe43ab7c1.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
MOLECULAR DETECTION OF ANAPLASMA MARGINALE IN THE EGYPTIAN WATER BUFFALOES (BUBALUS BUBALIS) BASED ON MAJOR SURFACE PROTEIN 1α
247
252
EN
MAHMOUD
D.
ELHARIRI
Departments of Microbiology, Faculty of Veterinary Medicine, Cairo
University, Giza 12613, Egypt.
REHAB
A.
ELHELW
Departments of Microbiology, Faculty of Veterinary Medicine, Cairo
University, Giza 12613, Egypt.
DALIA
A.
HAMZA
Departments of Zoonosis, Faculty of Veterinary Medicine, Cairo
University, Giza 12613, Egypt.
DOAA
E.
SOLIMAN
Department of Entomology, Faculty of Science, Ain
Shams university, Cairo 11566, Egypt.
10.21608/jesp.2017.77758
Anaplasmosis is an infectious, non-contagious disease caused by the rickettsial pathogen Anaplasma marginale. The organism is globally distributed and infects erythrocytes, resulting in anemia, jaundice, fever, abortions and death. Once infected, animals remain carriers for life. In developing countries anaplasmosis is of great economic losses as it is highly endemic. The present study was designed to determine the prevalence of A. marginale in blood samples of buffaloes since they are important reservoir hosts for A. marginale and can serve as a source of infection for tick. A total of 150 buffalo blood samples was randomly collected from four governorates and was analyzed using PCR assay based on msp1α. Anaplasma marginale DNA was detected in 69.3% (104/150) of the sampled buffaloes, and 86.6% (130/150) of collected<br />ticks. As anaplasmal infection is endemic in Egypt, it was recommended screening herds to detect A. marginale even when the signs and symptoms of infection were not visible.
Egypt,Anaplasma marginale,buffaloes,Ticks,msp1α gene,PCR
https://jesp.journals.ekb.eg/article_77758.html
https://jesp.journals.ekb.eg/article_77758_835f35cb001cf2341f703b551dea5591.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
AN INNOVATIVE REPURPOSING OF MEFLOQUINE; ASSESSMENT OF ITS THERAPEUTIC EFFICACY IN TREATING CRYPTOSPORIDIUM PARVUM INFECTION OF BOTH IMMUNOCOMPETENT AND IMMUNOCOMPROMIZED MICE
253
262
EN
NAGWA
SHABAN MOHAMED
ALY
Department of Parasitology, Faculty of Medicine, Benha University, Egypt.
RABAB
FAWZY
SELEM
Department of Parasitology, Faculty of Medicine, Benha University, Egypt.
RABAB
SAYED
ZALAT
Department of Parasitology, Theodore Bilharz Research Institute,
Imbaba P. O. Box 30, Giza, Egypt.
HEBA
KHALIL
Departments of Pathology, Theodore Bilharz Research Institute,
Imbaba P. O. Box 30, Giza, Egypt.
BOSHRA
EL-SAYED TALHA
HUSSIEN
Department of Tropical Medicine, Faculty of
Medicine, Tanta University, Egypt.
10.21608/jesp.2017.77763
Cryptosporidium parvum is a protozoan parasite can affect humans, worldwide, causing asymptomatic infections or diarrheal disease, which may be life-threatening in immunocompromised and neonatal individuals. Mefloquine is one of the most promising anti-parasitic drugs. The present report aimed to study the in-vivo efficacy of Mefloquine when applied inimmunocompetent and immunocompromised cryptosporidiosis-infected mice groups, each of the them was subclassified into the following groups :non-infected non-treated group (normal control), infected non-treated group (infected control), nitazoxanide treated group and Mefloquine treated group. One week post infection, treated groups received either Nitazoxanide (100mg\Kg daily for 5 days) or single dose of Mefloquine (400mg/ Kg). Two weeks post treatment, all mice were scarified. Stool samples and intestinal histopathological specimens were examined. For both drugs, immunocompetent groups showed better parasitological clearance than immunocomporomized one. Cryptosporidium oocyst reduction rates with Nitazoxanide (NTZ) and Mefloquine were 53.3%, and 61.6% respectively in the immunocompetent groups. The corresponding rates in immunocompromised groups were 49.93% and 60.03%.for NTZ and Mefloquine respectively. A single dose of Mefloquine treatment (400mg/kg) resulted in higher oocyst reduction rates than the approved anti-cryptosporidiosis drug (Nitazoxanide with five days application regimen). The histopathological study supported the parasitological findings as mefloquine treated mice tissue showed mild to moderate inflammatory changes while that of other groups ranged from moderate to severe alterations in the mice tissue. These results showed that Mefloquine which is FDA approved, already marketed and commercially available on a global scale has an excellent anti parasitic activity against C. parvum infection with single dose application; which saves time, cost and efforts to<br />search for additional or alternative drugs for treating cryptosporidiosis. More large scale studies are needed to illustrate its dose response relationship using multiple doses regimens, performance and limitations on immunocompromized population, synergistic effect with already approved drugs , mechanism of action on Cryptosporidium parasite and its possible role in chemoprophylaxis specially for high risk individuals.
Cryptosporidium parvum,Mefloquine,Nitazoxanide,In-Vivo
https://jesp.journals.ekb.eg/article_77763.html
https://jesp.journals.ekb.eg/article_77763_5b62eeaa221ddbf72c46b3b829a2ddf2.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
CHANGES IN PROTEIN CONTENT OF ARGAS (PERSICARGAS) PERSICUS (ACARI: ARGASIDAE) OVARY AFTER TREATMENT WITH ALLIUM SATIVUM EXTRACT
263
272
EN
OLA
H.
ZYAAN
Department of Entomology, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.
SHIMAA
S.
AHMED
Department of Entomology, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.
MOHAMED
A.
ABDOU
Department of Entomology, Faculty of Science, Ain Shams University,
Cairo 11566, Egypt.
10.21608/jesp.2017.77770
Argas (Persicargas) persicus is a parasitic feeder on blood of poultry birds. After each blood meal a female is capable of producing a large numbers of eggs, the hatched larva remains attached to its bird host for seven or more days and draw numerous bulky blood meals. The ovary is the main factory part for the oogenesis (production of eggs). The targeting treatment with ecofriendly acaricide is required to disrupt the normal physiology of the ovary. This study aimed to analyse and quantify total and fractionated protein in the ovary of A. persicus females treated with Allium sativum extracts from fresh garlic bulbs. Group of engorged females were topically treated with garlic extract at 1.44% concentration, and another group with water (control). All females were crossed independently with normal males. In order to analyze the proteins of the ovary, untreated females were dissected 0, 1, 2, 3, 4, 5, 6, 7, & 20 days after feeding (DAF), represented<br />different physiological states of tick and the treated females were dissected 3, 4, 5, 6, 7, & 20 DAF. The ovaries were homogenated and subjected to standard total and fractionated protein techniques. The protein concentration in ovary was significantly higher (P<0.05) in controls than in treated ones. Fractionated protein showed five characteristic bands to vitellogenesis process in controls. They reached highest concentration level at 7th days after feeding (DAF). These five bands disappeared in the treated samples except for one band in the corresponding time.
Argas (Persicargas) persicus,Allium sativum (garlic bulbs extracts),Vitellogenesis,Total protein,SDS-PAGE protein
https://jesp.journals.ekb.eg/article_77770.html
https://jesp.journals.ekb.eg/article_77770_a4b7a6ece3e21d14eb6bc61cd589d7e3.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
AN OVERVIEW ON MALARIA IN SUB-SAHARAN WITH SPECIAL REFERENCE TO TANZANIA
273
292
EN
MAHFOUZ
AHMAD
AL-AGROUDI
Military Medical Academy, Egypt.
LAILA
ABD EL-MAWLA
MEGAHED
Military Medical Academy, Egypt.
LAWRENCE
TIA
BANDA
Tanzania People’s Defence Forces.
TOSSON
ALY
MORSY
Department
of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
10.21608/jesp.2017.77777
Malaria is Anopheles-borne protozoan disease of worldwide distribution. Infection may result in a wide variety of symptoms, ranging from absent or very mild symptoms to severe disease and even death. Malaria can be categorized as uncomplicated or severe. It is a curable disease if diagnosed and treated promptly and correctly. All the clinical symptoms associated with malaria are caused by the asexual erythrocytic or blood stage parasites. When the parasite develops in<br />the erythrocyte, numerous known and unknown waste substances such as hemozoin pigment and other toxic factors accumulate in the infected red blood cell. These are dumped into the bloodstream when the infected cells lyse and release invasive merozoites. The hemozoin and other toxic factors such as glucose phosphate isomerase (GPI) stimulate macrophages and other cells to produce cytokines and other soluble factors which act to produce fever and rigors and probably influence other severe pathophysiology associated with malaria. After infective bite by Anopheles-vector, a period of time (incubation period) goes by before the first symptoms appear. Incubation period in most cases varies from 7 to 30 days. The shorter periods are most frequently with P. falciparum and the longer ones with P. malariae. Antimalarial drugs taken for prophylaxis by travelers can delay the appearance of malaria symptoms by weeks or months, long after the traveler has left the malaria-endemic area. This can happen particularly with P. vivax and P. ovale, both of which can produce dormant liver stage parasites; the liver stages may reactivate and cause disease months after the infective mosquito bite. Besides, malaria-co-infection with HIV/AIDS and others is another serious issue.
Africa,Sub-Sahara,Malaria,Tanzania
https://jesp.journals.ekb.eg/article_77777.html
https://jesp.journals.ekb.eg/article_77777_883b3b7c32cf469b459c425f190e71bb.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
EVALUATION OF THE PROTECTIVE VALUE OF SOME TOXOCARA VITULORUM ANTIGENS IN RATS
293
301
EN
OLFAT
A.
MAHDY
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University,
Giza, Egypt.
WAHEED
M.
MOUSA
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University,
Giza, Egypt.
SAHAR
Z.
ABDEL-MAOGOOD
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University,
Giza, Egypt.
SHIMAA
ABDEL-RADI
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University,
Giza, Egypt.
10.21608/jesp.2017.77780
Toxocara vitulorum is a nematode parasite of small intestine of buffalo, particularly young age calves, causing high morbidity and mortality. The control of this infection produces considerable economic benefits. Immunization of Wistar rats with different T. vitulorum antigens; (execratory/ secretory antigen (ESAg), peri-enteric fluid antigen (peAg) and embryonated eggs antigen (EEAg)) were evaluated for induction of protection against a challenge oral infection with embryonated eggs of T. vitulorum. It was found that peAg induce the highest protection level (100%) followed by ESAg<br />(96.4%-97.5%) then EEAg (93.7%-96.7%). Mean number of larvae extracted from immunized rats was significantly (P≤ 0.05) lower than that extracted from the control non-immunized one. The number of larvae collected after digestion of the liver at 3rd day post challenge (dp.c.) was decreased from 998.5 in control rats to 26 and 41.5 in rats immunized with ESAg and EEAg respectively. In the same time no significant (P≤0.05) difference in size of larvae extracted from immunized and control positive rats. For conclusion, the study demonstrated the value of rats as experimental model for investigation T. vitulorum infection. It characterized PeAg as a valuable immunogenic and protective antigen in minimize the infection by T. vitulorum between mother and calves in infected farms.
Toxocara vitulorum,Rats,Experimental study,ELISA
https://jesp.journals.ekb.eg/article_77780.html
https://jesp.journals.ekb.eg/article_77780_aa089b6a752e6d29f5b666d69b25e55a.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
MOLECULAR DIAGNOSIS OF CRYPTOSPORIDIUM SPP. VERSUS MICROSCOPY IN DIARRHEIC PATIENTS IN CAIRO
303
308
EN
OLFAT
M.
ELMATRAWY
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo
University, Egypt.
MARWA
A.
HASSAN
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo
University, Egypt.
SALWA
M.
MORSY
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo
University, Egypt.
JOSE
M.
RUBIO
Malaria & Emerging Parasitic Diseases Laboratory.
AYMAN
A.
EL-BADRY
National
Microbiology Centre, Institute de Salud Carlos III Madrid, Spain, and Department
of Microbiology-Medical Parasitology Section, Faculty of Medicine, University of
Dammam, Dammam, Saudi Arabia.
10.21608/jesp.2017.77781
Cryptosporidium spp. is considered one of the most common diarrhea-causing protozoa. This cross-sectional study was designed for molecular detection of Cryptosporidium spp., comparing results with microscopy using Acid Fast (AF) staining, and to determine predominance of cryptosporidiosis according to patients’ age and gender. Methodology: Stool samples were collected from 150 diarrheic patients attending outpatient clinics of Kasr Al-Ainy School of Medicine, Cairo University. Samples were examined microscopically by direct wet mount before and after sample concentration, and then subjected to copro-PCR assays. The results showed that out of 150 samples subjected to nPCR, only 9 (6%) samples were<br />positive for Cryptosporidium spp. and of those, 2 (1.3%) were positive by microscopy using AF stain. Microscopy showed high specificity but lower sensitivity (22%) compared to nPCR. Six of the 9 molecularly positive cases (66.7%) were children under 9 years and predominantly females with statistical significance.
patients,Cryptosporidium spp,AF stain,nested PCR
https://jesp.journals.ekb.eg/article_77781.html
https://jesp.journals.ekb.eg/article_77781_c27e55aa06ffcbb3553451b64ebd84fc.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
AN INTERVENTION PROGRAM ON BLOOD PROTOZOA ACQUIRED BY NEEDLE STICK INJURY AND INFECTION CONTROL
309
322
EN
AMR M.
EL-SAYED
ABDEL-MOTAGALY
Military Medical Academy, Cairo 11291, Egypt.
ABEER
MOHAMMAD ABDALLAH
IBRAHIM
Military Medical Academy, Cairo 11291, Egypt.
TOSSON
A.
MORSY
Faculty of Medicine,
Ain Shams University, Cairo 11566, Egypt.
10.21608/jesp.2017.77782
Needle stick injuries are a common event in the healthcare environment. When drawing blood, administering an intramuscular or intravenous drug, or performing other procedures involving sharps, needle can slip and injure the healthcare worker. This allows for transmission of pathogens. Injuries also occur during needle recapping or failure to place used needles in the sharps containers. Also, lack of access to or failure to use appropriate personal protective equipment can cause needle stick injuries. Night shifts also put practitioners at risk for needle stick injuries. The study assessed the effect of an intervention program for nursing staff knowledge, performance and attitude related to blood protozoa acquired by needle stick injury. Design an interventional study (pre-post study) was used for 90 nursing staff, ten of them pilot study were excluded. The study tools composed of educational needs assessment tool, knowledge questionnaire sheet (pre/post-test), observation check list and attitude Results showed significant improvements in nursing staff knowledge, performance and attitude.
Nurses,Intervention,Blood protozoa,needle stick injury,Infection control
https://jesp.journals.ekb.eg/article_77782.html
https://jesp.journals.ekb.eg/article_77782_1317029940c49a3e8c174eb5ab8d1767.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
ASSOCIATION BETWEEN POST TREATMENT EXISTENCE OF HCV/ RNA POSITIVE STRAND AND/OR NEGATIVE STRAND IN THE PBMNCS AND HCV RELAPSE
323
330
EN
AMGAD
ALI ABD ELHADI
ELZAHABY
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
GAMAL
MOHAMMAD MOHAMMAD
SOLIMAN
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
MOHAMMAD
ABDALLAH
KORANY
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
DIAA
MOHAMED
ELTIBY
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
MOSTAFA
KAMEL
EL AWADY
Department of Microbial Biotechnology, National Research Center, Cairo, Egypt.
10.21608/jesp.2017.77783
Hepatitis C virus (HCV) has been found to infect peripheral blood mononuclear cells (PBMCs), using them as a reservoir, which might contribute to development of resistance to treatment. The study evaluated the predictive value of existence of HCV RNA in PBMCs of chronic hepatitis C patients and its association with HCV seroconversion following the completion of therapy with direct antiviral agents (DAAs). Methods: 58 Egyptian patients were included with chronic HCV infection<br />who achieved negative serum HCV RNA after completion of therapy with DAAs. HCV RNA in PBMCs and serum was investigated at the end of treatment and 12 weeks later. At the end of treatment patients were subdivided into three groups. GA: included 23 patients with negative (positive & negative strands of HCV RNA) in PBMCs. GB: included 24 patients with positive (positive strand) of HCV RNA in PBMNCs. GC: included 11 patients with positive (positive &<br />negative strands of HCV RNA) in PBMCs. The results showed that 16 relapsers out of 58 patients (27.59 %). GA only one patient out of 23 (4.3 %) failed to achieve SVR, so the absence of HCV RNA in PBMNCs is associated with high SVR rate (95.7%).GB 10/24 patients failed to achieve SVR, so the presence of HCV RNA positive strand in PBMNCs is associated with high relapse rate (41.7 %), GC 5/11 patients failed to achieve SVR, where the presence of HCV RNA (negative replicative and positive genomic strands) in PBMNCs is associated with higher rate of relapse (45.45%).
patients,HCV,PBMNCs,relapse,DAAs
https://jesp.journals.ekb.eg/article_77783.html
https://jesp.journals.ekb.eg/article_77783_4817248eb8540c993b90015ba30073ab.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
WHAT IS THE HISTORY OF MAGGOT THERAPY?
331
332
EN
TOSSON
A.
MORSY
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566,
Egypt.
10.21608/jesp.2017.77784
https://jesp.journals.ekb.eg/article_77784.html
https://jesp.journals.ekb.eg/article_77784_07e6f460a2e47c47194444f9cd86bd5c.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
CRYPTOSPORIDIUM AMONG ASYMPTOMATIC FOOD HANDLERS
333
340
EN
AFAF
A.
TAHA
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
FATEN
A.
MOHAMMED
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
SABAH
M. A.
MOHAMED
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
10.21608/jesp.2017.77785
Cryptosporidiosis in food handlers is an important public health problem. Cryptosporidium infection is transmitted by ingestion of Cryptosporidium oocysts through consumption of a contaminated food or water. Workers in food sector can play a role in cryptosporidiosis outbreak. This study aimed to detect the existence of Cryptosporidium among asymptomatic food handlers using microscopical examination and commercially available antigen capture ELISA. Stool samples collected from asymptomatic 237 food handlers, aged from 17-50 years old and worked at different branches of food sector within Zagazig and its surrounding. Fecal samples were examined microscopically to detect Cryptosporidium oocysts by modified Ziehl-Neelsen stain (MZN) and by ELISA for detection of Cryptosporidium copro-antigen. Among 237<br />food handlers asymptomatic cryptosporidiosis has been detected in 10 (4.2%) by MZN, 12 (5%) by ELISA and 13(5.5%) by both techniques. Out of 13 asymptomatic infected food handlers, three of them worked as fruits/vegetables sellers, two at restaurant, three butchers, two as fast food workers, one at Canteen, Café and two at supermarket. These findings indicate that food handlers can be a source of cryptosporidiosis infection. Thus, searching for the existence of asymptomatic cryptosporidiosis food handlers and taking the required measures in case of its determination are helpful in prevention of probable morbidity and protection of consumer health.
Asymptomatic,Cryptosporidium,Food handlers
https://jesp.journals.ekb.eg/article_77785.html
https://jesp.journals.ekb.eg/article_77785_d8693355c9dfe508d25e3a727b72f8be.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
DESCRIPTION OF TWO DIGENEAN PARASITES LECITHOCHIRIUM PRIACANTHI YAMAGUTI, 1953 (HEMIURIDAE) AND PSEUDOPECOELOIDES ARTHERINOMORI AKEN'OVA ET AL, 2009 (OPECOELIDAE) INFECTING ANGUILLIDAE AND SERRANIDAE MARINE HOSTS IN EGYPT
341
348
EN
MANAL
EL-GARHY
Department of Zoology, Faculty of Science, Cairo University, Egypt.
NESMA
MOSTAFA
Department of Zoology, Faculty of Science, Cairo University, Egypt.
ALI
AL GHAMDI
Department
of Biology, College of Science, Al Baha University, Saudi Arabia.
SALWA
A. H.
HAMDI
Department of Zoology, Faculty of Science, Cairo University, Egypt.
KAREEM
MORSY
Department of Zoology, Faculty of Science, Cairo University, Egypt.
10.21608/jesp.2017.77786
The study described of two parasitic digenean species isolated from pyloric portion of stomach and middle part of their host intestines. Lecithochirium priacanthi Yamaguti, 1953 (Hemiuridae) & Pseudopecoeloides artherinomori Aken'Ova et al, 2009 (Opecoelidae) respectively infecting two marine fish, the European sea eel Anguilla anguilla (Anguillidae) captured from Alexandria City coast, Mediterranean Sea and the spotted coral grouper Plectropomus maculatus<br />(Serranidae) from coasts of Suez Gulf and Hurghada of the Red Sea, both species captured during the year 2015. The morphology and morphometric characterization were carried out by light microscopy. L. priacanthi characterized by elongated body measured 2.11±0.20mm (1.93-2.54) in length with tapered conical anterior and cylindrical wide posterior ends, supplied with a very short retracted tail with blunt end and two large oval testes. This species is unique by two<br />compact, lobed masses of vitellaria just posterior to ovary. P. artherinomori characterized by elongated slender body measured 1.8±0.02mm (1.4-2.20) in length with a ventral sucker larger than oral sucker situated on a very short peduncle formed wrinkles around sucker. Both species were recorded for the first time from these host species in Egypt.
Lecithochirium priacanthi,Hemiuridae,Anguilla Anguilla,Pseudopecoeloides artherinomori,Opecoelidae,Plectropomus maculates,light microscopy
https://jesp.journals.ekb.eg/article_77786.html
https://jesp.journals.ekb.eg/article_77786_bed8afdd47a570bd1785bb26fc991a62.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
COMPARISON BETWEEN AURAMINE PHENOL STAIN, MODEFIED ZIEHIL-NEELSEN AND ELISA FOR DETECTION OF INTESTINAL COCCIDI
349
354
EN
REDA
L.
EL GAMAL
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
MAHA
S.
BADAWEY
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
MARWA
A.
SALAMA
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
MAI
E.
DAHROG
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
10.21608/jesp.2017.77787
Coccidia had been implicated as the most important opportunistic parasites in patients with acquired immune deficiency syndrome. It is transmitted via the feco-oral route. The study aimed to compare the efficacy of microscopic tests with ELISA assay for detection of oocysts in feces. This study assisted the presence of Coccidia oocyst in fecal samples of 300 patients attending Zagazig University Hospital by using modified Ziehl-Neelsen stain (MZN) ,Auramine phenol stain (AP) and Enzyme-linked immunosorbent assay. Cryptosporidium infection rate was the highest (20.7%) among other Coccidia. Mixed infection of Cryptosporidium and Cyclospora either alone or with Cytoisospora and that of Cryptosporidium and Cyclospora with concomitant Microsporidia were recorded (9.7%, 1.7% and 5% respectively) by AP and (17.7, 6.7, 1 and 3.3 % respectively) by MZN. The specificity of modified Ziehl-Neelsen was 100% for both, while the sensitivity was 77.5%, while they were 100% and 100% respectively for AP, and 100% and 91.9% for ELISA. Conclusion, this study shows that Auramine phenol is a simple fluorescent staining, promising technique in diagnosis of intestinal Coccidia as it has high sensitivity and specificity and it is less time-consuming method.
patients,Stool staining techniques,Apicomplexa,Cryptosporidium
https://jesp.journals.ekb.eg/article_77787.html
https://jesp.journals.ekb.eg/article_77787_ea36938807b6808f38cc224b9e8b5e13.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
SEROPREVALENCE AND RISK FACTORS OF TOXOPLASMA GONDIIINFECTION AMONG PREGNANT WOMEN IN AD-DAWADIMI GENERAL HOSPITAL, KINGDOM OF SAUDI ARABIA
355
362
EN
ABDULLAH
D.
ALANAZI
Department of Biological Sciences, Faculty of Science and Humanities, Shaqra
University, P.O. Box 1040, Ad-Dawadimi 11911, Saudi
Arabia.
MOHAMMED
S.
ALYOUSIF
Department of Zoology, College of
Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi
Arabia.
SULIMAN
A.
ALOMAR
Department of Zoology, College of
Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi
Arabia.
IBRAHIM
O.
ALANAZI
The National Center
for Genomic Technology, King Abdulaziz for Science and technology, P.O. Box
6086, Riyadh 11442, Saudi
Arabia.
HAMDAN
I.
AL-MOHAMMED
Department of Microbiology and Medical Parasitology,
Faculty of Medicine, King Faisal University, P.O. Box 400, AlAhsa 31982, Saudi
Arabia.
10.21608/jesp.2017.77788
The present study investigated the seroprevalence of two antibodies (anti-T. gondii IgG and IgM) among the pregnant women in Ad-Dawadimi City, along with possible risk factors associated with Toxoplasma gondii. A total of 461 pregnant women were enrolled at various stages of pregnancy, either asymptomatic or having clinical signs and symptoms suggestive of toxoplasmosis (fever, lymphadenopathy, hepatomegaly, splenomegaly, and rash). A short face to face questionnaire interview for pregnant women was carried out to obtain information regarding T. gondii infection. Out of the 461 blood samples tested, 189 (40.9%) were seropositive for specific anti-T. gondii IgG antibodies. But, none of the women had positive IgM results. Seroprevalence of T. gondii antibodies was high among pregnant women and the prevalence showed a corresponding increase as the age of the pregnant women increases. This study shows that<br />Toxoplasmosis is an endemic disease in Ad-Dawadimi city. Thus, an awareness creation program on the modes of transmission and prevention of T. gondii among women of child bearing age in general and pregnant women in particular should be organized during the antenatal care follow up.
Saudi Arabia,Ad-Dawadimi City,toxoplasma gondii,seroprevalence,ELISA
https://jesp.journals.ekb.eg/article_77788.html
https://jesp.journals.ekb.eg/article_77788_5fc570891c8ec1ac15070fd44edb8c74.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
SCHSITOSOMA MANSONI CO-INFECTION WITH HEPATITIS C VIRUS IS ASSOCIATED WITH INCREASED INTERLEUKIN-28B PLASMA LEVELS IN EGYPTIAN POPULATION
363
374
EN
SAMIRA
Z. A.
EID
Department of Parasitology and Blood Research, National Organization for Drug
Control and Research (NODCAR), Cairo12654, Egypt.
NOURTAN
F.
ABDELTAWAB
Department of Microbiology
and Immunology, Faculty of Pharmacy, Cairo University, Cairo11562, Egypt.
SAMUEL
T.
MELEK
Department of Parasitology and Blood Research, National Organization for Drug
Control and Research (NODCAR), Cairo12654, Egypt.
MAGDY
A.
AMIN
Department of Microbiology
and Immunology, Faculty of Pharmacy, Cairo University, Cairo11562, Egypt.
10.21608/jesp.2017.77789
Intestinal schistosomiasis and hepatitis C viral (HCV) infections are endemic in Egypt with coinfections leading to increased severity of liver diseases. Previous studies characterized the immune responses to treatment in co-infection. However, little is known about the levels of interleukin 28B (IL-28B) in co-infection and its relation to endogenous gamma interferon (IFN-γ) levels. Therefore, a case-control study was performed comparing levels of IL-28B in relation to endogenous IFN-γ in Schistosoma mansoni / HCV co-infected Egyptian patients compared to HCV mono-infected patients. Patients attending Kasr Al-Aini Hospital, Cairo from 2012–2014 were recruited. Subjects recruited were S. mansoni/HCV co-infected (n=22), treatment-naïve chronic HCV-4 (n=50), and healthy controls (n=35). Clinical history and liver function markers were determined for each participant. IL-28B and IFN-γ plasma levels were assayed for all participants by ELISA and HCV load was quantified using Real-Time PCR. Plasma antischistosomal antibody titers were assayed along with viable egg identification in feces. Patients with high HCV viral load had significantly higher IFN-γ and IL-28B levels whether suffering from HCV mono- or co-infection. Moreover, IFN-γ levels were positively associated with IL-<br />28B plasma levels in HCV mono- and co-infection. The IL-28B levels were significantly higher in S. mansoni/HCV co-infected than HCV mono-infection patients (p < 0.05).Data suggested that co-infection of HCV with S. mansoni affected IL-28B levels and IL-28B plasma levels might prove with sufficient further studies to be an effective prognosis biomarker for S. mansoni / HCV co-infection in the Egyptian population.
Hepatitis C virus,Schistosoma mansoni,co-infection,IFN-γ,IL-28B,biomarkers,tropical diseases
https://jesp.journals.ekb.eg/article_77789.html
https://jesp.journals.ekb.eg/article_77789_f830a8315e03217584c3cd653d5512d5.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
INTESTINAL PARASITES IN CHILDREN RECEIVING CHEMOTHERAPY
375
380
EN
HANAA
AHMED
EL-HADY
Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
NOHA
SAMMER
AHMED
Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
MOHAMED
AHMED ALI
TAHA
Department
of Medical Parasitology, Faculty of Medicine, Al Azhar University, Egypt.
NASSER
MOHAMED
ABD EL-KAREEM
Department
of Medical Parasitology, Faculty of Medicine, Al Azhar University, Egypt.
RAFAAT
ABDELAAL
BAKHEET
Department
of Oncology, Faculty of Medicine, Sohag University, Egypt.
10.21608/jesp.2017.77790
Serious complications associated with some parasitic diseases in immunosuppressed patients. An extreme course might be connected with noteworthy morbidity and mortal sin in children having intestinal parasitic infections and getting chemotherapy for management from threatening tumors. This study pointed at figuring out the prevalence and type of intestinal parasites over stool specimens of children receiving chemotherapy contrasted with healthy children. A cross-sectional survey was calculated among 100 children receiving chemotherapy in Sohag Oncology Institute and 100 apparently healthy children as a control group. Stool examination by direct method and concentration by formol-ether sedimentation, then staining with Kinyoun’s modified acid-fast stain were done. Among those children receiving chemotherapy, 94% were diagnosed positive for parasitic infections, in contrast to 35% in the control group. Parasites that were detected in children receiving chemotherapy and healthy control group respectively were Cryptosporidium sp.(45% vs.10%), Giardia lamblia (19% vs. 7%), Entamoeba histolytica (14% vs. 4%). Statistically significant differences in these parasitic infections were detected between the two studied groups (p-value < 0.001), while infections with Entamoeba coli (12% vs. 4%), Hymenolepis nana (4% vs. 4%), Schistosoma mansoni (0% to 2%) were statistically not significant. Parasitic infection is common among children receiving chemotherapy. In order to get the suitable management clinicians treating children receiving chemotherapy should make mindful about these infections.
children,Chemotherapy,Cryptosporidium sp,Giardia lamblia,Entamoeba histolytica
https://jesp.journals.ekb.eg/article_77790.html
https://jesp.journals.ekb.eg/article_77790_bee467bcb3f7181927be799dd7acb35d.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
SEROPREVALENCE OF TOXOPLAMA GONDII AMONG PRIMIGRAVIDA WOMEN AND THEIR NEONATES IN SOHAG GOVERNORATE, EGYPT
381
388
EN
SALAH
M. M.
HUSSEIN
Departments of Parasitology, Faculty of Medicine, Al-Azhar University, Assuit Governorate, Egypt.
AHMED
SAAD
ELSHEMY
Departments of Pediatric, Faculty of Medicine, Al-Azhar University, Assuit Governorate, Egypt.
MOHAMED
MOUSA
ABD EL-MAWGOD
Departments of Public Health,
Faculty of Medicine, Al-Azhar University, Assuit Governorate, Egypt.
ALI
S.
MOHAMMED
Departments of Clinical Pathology,
Faculty of Medicine, Al-Azhar University, Assuit Governorate, Egypt.
10.21608/jesp.2017.77791
Infections with the Toxoplasma gondii parasite (T. gondii) occur worldwide and affect about one third of humanity. The study determined the prevalence of T. gondii among primigravidae Women and vertical transmission of T gondii in their newborns. Socio-demographic data and potential predisposing risk factors were studied using structured questionnaire. Venous blood samples (350 samples) were collected and tested for IgM & IgG anti-Toxoplasma gondii antibodies by enzymelinked immunosorbent assay (ELISA) during pregnancy period and at birth for primigravidae and their neonates, PCR test used as confirmatory test for IgM positive samples. Of the 350 pregnant women included in the study 165 (47.1%) cases were IgG seropositive of them 33 (9.4) cases were IgM seropositive. 185 (52.9%) cases were seropositive for Toxoplasma-specific IgG and IgM antibodies. At birth serological study of primigravidae and their neonates revealed that 142 (40.5%) cases were chronically infected, 25 (7.1%) cases showed active infection and 183 (52.2%) cases<br />were susceptible to infection; with prevalence of vertical transmission of 20% (5/25). Rural areas, contact with cats and ingestion of raw milk were statistically significantly associated with higher infection rates. Pigeon, sheep meat and ingestion of raw milk and egg represent the main source of infection in the studied area.
toxoplasma gondii,Primigravidae,Neonates,seroprevalence,ELISA assay,PCR,Sohag,Upper Egypt
https://jesp.journals.ekb.eg/article_77791.html
https://jesp.journals.ekb.eg/article_77791_2b2332076d4fba14cfa78776bc3602a0.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
TAENIA SAGINATA IN MAN AND CYSTICERCOSIS IN CATTLE AND BUFFALOES IN ASWAN GOVERNORATE, EGYPT
389
394
EN
AHMED
K.
DYAB
Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.
MOHAMMED
E.
MARGHANY
Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.
RAGAA
A.
OTHMAN
Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.
MAHMOUD
A.
AHMED
Department of Parasitology, Faculty of Veterinary Medicine, Aswan, Egypt.
OSAMA
H.
ABD-ELLA
Department
of Medical Parasitology, Faculty of Medicine, South Valley University, Egypt.
10.21608/jesp.2017.77792
Taeniasis is an important foodborne parasitic disease worldwide. This study determined the presence of cysticercosis in cattle and buffaloes and discussing their public health importance in Aswan Governorate, Upper Egypt. A total number of 45780 cattle, 223 buffaloes, were examined during the extended period from August 2015 till the end of July 2016. The existence of Cysticercus bovis infection among slaughtered Cattle and Buffaloes was studied at three different slaughterhouses (Aswan, Draw and Al-Sharq Al-Awsat) in Aswan Governorate. C. bovis in slaughtered cattle and buffaloes was (7.5% & 1.34%), respectively. Cattle and buffaloes infected with C. bovis it was for young and old animals, respectively (7.5% &12.12%); (0.51% & (7.14%). C. bovis in slaughtered cattle was commonly found in the tongue muscles (70.46%), Heart (2.8%), masseter muscles (0.43%) and gluteal muscles (0.29%). While for buffaloes heart (100%) was the most common site infected with C.bovis .Moreover, Viable C.bovis was (86%) in slaughtered cattle and buffaloes. Taeniasis was detected in (0.4%) of examined patients with gastroenteritis. The infection among male is (1.5%) while it not detected in females. The results confirmed that cysticercosis is endemic among cattle and buffaloes in Aswan Governorate. Meat inspection is a must.
Egypt,Aswan,Foodborne parasite,Cysticercosis,Taeniasis,Zoonosis
https://jesp.journals.ekb.eg/article_77792.html
https://jesp.journals.ekb.eg/article_77792_cf03242177415233ac58507bb50eae05.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
EVALUATIVE STUDY OF THE ERCP UNIT AT SAYED GALAL UNIVERSITY HOSPITAL IN COMPARISON WITH OTHER CENTERS
395
401
EN
YASSER
FOUAD
ALKEELANY
Department of Tropical Medicine, Faculty of Medicine, Al Azhar University, Nasr City,
Egypt.
MOHAMED
M.
ELAWADY
Department of Tropical Medicine, Faculty of Medicine, Al Azhar University, Nasr City,
Egypt.
DIAA
MOHMAED
ELTIBY
Department of Tropical Medicine, Faculty of Medicine, Al Azhar University, Nasr City,
Egypt.
SAYED
FAROUK
MOHAMED
Department of Tropical Medicine, Faculty of Medicine, Al Azhar University, Nasr City,
Egypt.
GALAL
ABD ALHAMED
ABUFARAG
Department of Tropical Medicine, Faculty of Medicine, Al Azhar University, Nasr City,
Egypt.
10.21608/jesp.2017.77793
Endoscopic retrograde cholangiopancreatography (ERCP) is a technique that combines the use of endoscopy and fluoroscopy to diagnose and treat certain problems of the biliary or pancreatic ductal systems. A successful ERCP requires the co-ordination and co-operation of dedicated and committed team of endoscopists, nurses, and assistants, as well as an organized and functioning unit. It takes many years to learn, and repeated practice, in order to master the skill<br />of ERCP and to do it safely. The study evaluated the ERCP unit of Sayed Galal University according to safety, efficacy and complications in diagnosis and management of biliary obstruction by a retrospective study in comparison with other centers. This study was conducted on 318 patients with obstructive jaundice who fulfilling the designed inclusion criteria. The study was carried out in outpatient clinics of Tropical Medicine Department Al-Azhar University Hospitals (Sayed Galal Hospital), during the period from January 2012 to December 2016. The results showed the cannulation gave 94.3% good findings as 300 cases were successfully relieved the obstruction using either ES only, ES and stone extraction or stent application The side effects were 16 (5%) cases making the ERCP relatively a safe procedure; however, with potential for<br />severe life-threatening complications.
Egypt,patients,Endoscopic retrograde cholangiopancreatography
https://jesp.journals.ekb.eg/article_77793.html
https://jesp.journals.ekb.eg/article_77793_6b87a6e351c926b57e29f0b13fe077e1.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
MOLECULAR DETECTION OF BORRELIA SP. IN ORNITHODOROS SAVIGNYI AND RHIPICEPHALUS ANNULATUS BY FlaB GENE AND BABESIA BIGEMINA IN R. ANNULATUS BY 18S rRNA GENE
403
414
EN
MOSTAFA
I.
HASSAN
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
HANAN
S. M.
GABR
Department of Zoology and Agricultural Nematology, Faculty of Agriculture,
Cairo University, Giza, Egypt.
SOBHY
ABDEL-SHAFY
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt.
KOTB
M.
HAMMAD
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
MOSTAFA
M.
MOKHTAR
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
10.21608/jesp.2017.77795
This study determined the ability of the soft tick, Ornithodoros savignyi and the hard tick, Rhipicephalus (formerly Boophilus) annulatus to serve as carrier for new genotypes of Borrelia and Babesia. Ornithodoros savignyi, was collected in the summer, 2015 from camel market at Shalatein, Red Sea Governorate, while R. annulatus was collected from cows at Salhia, Sharkia Governorate and Banha, Qalyoubia Governorate. Hemolymph smears were prepared and stained with Giemsa stain and examined by light microscope (LM) for the presence of spiral form of Borrelia and vermicule stage of<br />Babesia. The tick specimens that revealed positive infection with either Borrelia or Babesia by LM were screened by PCR using flaB gene for Borrelia and 18S rRNA gene for Babesia. The obtained amplicons were sequenced, registered in GenBank and the phylogenetic trees for the obtained sequences were constructed. Results showed that spirochetes (Borrelia) were found in O. savignyi and R. annulatus, while the vermicule form of Babesia was found in R. annulatus only. The PCR amplified Borrelia and Babesia at 350 &50 bp, respectively. The obtained amplicons were recorded in GenBank<br />with accession number MF084762, MF084761 & MF004418 for Borrelia sp. in O. savignyi, Borrelia sp. in R. annula-tus and B. bigemina in R. annulatus, respectively. The genotype of Borrelia sp. recorded in O. savignyi is very close to B. burgdroferi that causes Lyme disease in human. But, genotype of Borrelia sp. recorded in R. annulatus is close to B. theileri that causes a mild disease in animals. Genotype of Babesia in R. annulatus was 100% identical with B. bigemina that recorded before.
FlaB,18S rRNA,Borrelia,Babesia,Ornithodoros savignyi,Rhipicephalus annulatus
https://jesp.journals.ekb.eg/article_77795.html
https://jesp.journals.ekb.eg/article_77795_b512b9295a19ae43a7470e36b753cca9.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
A MINI-REVIEW ON SKEETER SYNDROME OR LARGE LOCAL ALLERGY TO MOSQUITO BITES
415
424
EN
AMR
M. EL-SAYED
ABDEL-MOTAGALY
Military Medical Academy, Cairo 11291, Egypt.
HANAA
MAHMOUD
MOHAMAD
Military Medical Academy, Cairo 11291, Egypt.
TOSSON
A.
MORSY
Department of Parasitology,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
10.21608/jesp.2017.77796
Skeeter Syndrome is an allergy to mosquito saliva secreted while taken a human blood meal. It is present with extreme swelling, itching, blistering, infection, fever and general malaise, some cases develop asthma and cellulitis and even threatening anaphylactic shock. Most people of all ages particularly small children, toddlers and seniors who suffer from skeeter syndrome experience a very extreme reaction showed some allergic reaction level, with itching and redness.<br />Sometimes, the swelling is painful and so extreme that the affected limb doubles in size, eyes swell shut, and the area feels hot and hard to the touch or the bite will blister and ooze.
Mosquito bite,Skeeter syndrome,Differential diagnosis,Treatment,prevention
https://jesp.journals.ekb.eg/article_77796.html
https://jesp.journals.ekb.eg/article_77796_5181ae9a3ae61b07e4ceff103e3ea745.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
PREVALENCE OF TICK-VECTORS OF THEILERIA ANNULATA INFESTING THE ONE-HUMPED CAMELS IN GIZA, EGYPT
425
432
EN
MOSTAFA
I.
HASSAN
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
HANAN
S. M.
GABR
Department of Zoology and Agricultural Nematology, Faculty of
Agriculture, Cairo University, Giza, Egypt.
SOBHY
ABDEL-SHAFY
Department of Parasitology and Animal
Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza,
Egypt.
KOTB
M.
HAMMAD
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
MOSTAFA
M.
MOKHTAR
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University,
Nasr City, Cairo, Egypt.
10.21608/jesp.2017.77797
Theileria are obligate intracellular protozoan parasites transmitted by hard ticks that cause severe and mild infections in their vertebrate hosts. The objective of the present study was to identify the possible tick vector of Theileria spp. and to determine the prevalence of camel theileriosis in Birqash market, Giza, Egypt. These camels are previously imported from Sudan and Somalia. A total of 298 nomadic one - humped camels in the study area were selected by simple random sampling technique during the period from December 2014 to November 2015. A total of 1540 ticks were collected, four ixodid tick species; Hyalomma dromedarii, Amblyomma lepidum, Amblyomma variegatum, and Rhipicephalus pulchellus were found on camels. It was observed that H. dromedarii recorded the highest prevalence (69%), followed by A. lepidum (8%), A. variegatum (3%) and R. pulchellus (2%). Using light microscope Hyalomma dromedarii was the most tick carrier of Theileria spp. which recorded 1.3% (20/1540) Theileria infection. The highly prevalence rates for all ticks were monitored during the summer season. The molecular detection of Theileria annulata in Geimsa-stain positive H. dromedarii ticks was performed by the PCR using primer set N516/N517 derived from the gene encoding the 30 kDa major merozoite surface antigen. This primer set amplified T. annulata in H. dromedarii at 750 bp. Thus the presence of H. dromedarii on camels in thefield have dangerous inclusive for animal health and to some extent humans. To reduce these<br />dangerous effects, strategic control methods could be used in the control of ticks.
Amblyomma, Hyalomma, Rhipicephalus, Ixodidae, Prevelance, Theileria,30KDa
https://jesp.journals.ekb.eg/article_77797.html
https://jesp.journals.ekb.eg/article_77797_2abe5f800c3b6784df04f55ef8681f7d.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
PREVALENCE OF REFLUX OESPHAGITIS IN EGYPTIAN PATIENTS WITH LIVER CIRRHOSIS
433
441
EN
ABDOU
MABROUK
EL-SHAFEI
Departments of Tropical Medicine, Faculty of Medicine,
Al-Azhar University, Cairo, Egypt.
DIAA
MOHAMMAD
EL-TIBY
Departments of Tropical Medicine, Faculty of Medicine,
Al-Azhar University, Cairo, Egypt.
SHERIF
ALI
ABDELAZIZ
Departments of Tropical Medicine, Faculty of Medicine,
Al-Azhar University, Cairo, Egypt.
AHMED
ATEF
ABDEL-FATAH
Departments of Tropical Medicine, Faculty of Medicine,
Al-Azhar University, Cairo, Egypt.
ABDULLAH
MUSTAFA
GAAFAR
Departments of Clinical Pathology, Faculty of Medicine,
Al-Azhar University, Cairo, Egypt.
10.21608/jesp.2017.77947
Reflux esophagitis (RE) is increasing in prevalence in Egypt. There are few studies on the prevalence and factors related to RE in patients with liver cirrhosis. This study determined the prevalence of RE in patients with liver cirrhosis and the possible related predictors of RE. This study was conducted on 150 patients with liver cirrhosis who underwent upper GI endoscopy for different indications. GERD, if present was classified according to Los Angeles Classification, Oseophageal biopsies taken to exclude Barret's oesophagus and microscopic reflux oesphagitis. The results showed that 36 patients had GERD (24 %). The most prevalent was GERD grade (B). Symptomatic GERD was highly prevalent in patients with liver cirrhosis, reported in about (48.67%). Heart burn was the chief symptom with a significant relationship between GERD and severity of the liver disease as assessed by Child-Turcotte-Pugh scoring system. A significant relationship between the severity of GERD and the degree of ascites was demonstrated, as GERD grade (C) was present more frequently in patients with marked ascites. The presence of GERD was significantly associated with the esophageal varices, which could be<br />amechanical factor contributing to esophageal dysmotility and predisposing to GERD, patients with no GERD by endoscopy (114) microscopically showing microscopic oesphagitis (29.8%), RE (36.8%), while patients with GERD by endoscopy(36) microscopically showing barrets oesphagus (22.3%),RE with mild activity (41.6%),RE with moderate activity (36.1%).
Egypt,patients,Reflux oesphagitis,Liver cirrhosis
https://jesp.journals.ekb.eg/article_77947.html
https://jesp.journals.ekb.eg/article_77947_80ef54f4f5409a9966594d22806bfdeb.pdf
The Egyptian Society of Parasitology
Journal of the Egyptian Society of Parasitology
1110-0583
2090-2549
47
2
2017
08
01
BEE VENOM LOADED CHITOSAN NANOPARTICLES AS TREATMENT FOR AMOEBIASIS IN MICE
443
458
EN
AMANY
EL SAYED
SABER
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
ABDELWAHAB
KHALIL
ABDELWAHAB
Department of
Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
AZZA
MOHAMED
EL AMIR
Department of
Zoology, Faculty of Science, Cairo University,Giza, Egypt.
MAMDOUH
IBRAHIM
NASSAR
Department of Entomology,
Faculty of Science, Cairo University, Giza, Egypt.
HUSSEIN
FOUAD
ZOHDI
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
10.21608/jesp.2017.77951
Entamoeba histolytica is an invasive parasite and results in intestinal inflammation and amebic liver abscess. Metronidazole is the main used drug for amoebiasis in both adults and children. However, metronidazole has some drawbacks such as having potential mutagenic effects in rats as well as the problem of the emergence of drug- resistance species makes the development of other safer drugs for amoebiasis is critical. In this study we assessed the ability of bee venom (BV) loaded chitosan (CS) nanoparticles to treat amoebiasis and that BV has antiamoebic effect as well as it is proved that orally administered BV loaded CS nanoparticles can be a more effective alternative drug for amoebiasis than subcutaneously injected BV.
Amoebiasis,Bee venom,Chitosan nanoparticles
https://jesp.journals.ekb.eg/article_77951.html
https://jesp.journals.ekb.eg/article_77951_cde6a5924e0ba4de12629e7ed99c7218.pdf