ORIGINAL_ARTICLE
CYTOKINES AND MOTHER SPOROCYSTS IN SUSCEPTIBLE AND RESISTANT BULINUS TRUNCATUS SNAILS INFECTED WITH SCHISTOSOMA HAEMATOBIUM
The presence of immunoreactive interleukin (IL-2), interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in addition to the citation of mother sporscytes in cephalopodal musculature in the susceptible and resistance Bulinus truncatus the specific intermediate host for the trematode Schistosoma haematobium were investigated,. Using ELISA tests, Results indicated that the concentration of IL-2-like activity in the susceptible and resistant snails decreased significantly after infection then persisted at low levels until the 4th week post exposure (WPE) in susceptible snails, while in resistant snails elevated during the second WPE, and returned to initial level at 3 and 4 WPE. Susceptible snails had low detectable levels of TNF-α and INF- like-activity after infection. However, the resistant snails had significant low levels of TNF-α and INF- like-activity from 3 WPE until the 4th WPE without any sign of normalization. Histological sections in the head- foot region of susceptible and resistance B. truncatus infected with S. haematobium, mother sporocysts exists from1 to 7(day post exposure) DPE, in the susceptible snail the mother sporocysts were found as single, multipleand mature types. No mother sporocysts were appear in the lip and mantle of the snail on 2, 5, 7 DPE and on 1-3 , 6 DPE respectively. In the resistant snails few mother sporocysts were found in the lip, mantle and tentacles. The results showed that schistosome-resistant Bulinus can be an alternative strategy for the control of schistosomiasis.
https://jesp.journals.ekb.eg/article_90379_fec2aa5f5fd949ad557493ef7af31d95.pdf
2014-08-01
285
293
10.21608/jesp.2014.90379
Bulinus truncatus
cytokine Schistosoma haematobium
Sporocyst
Susceptible
resistant
ABDEL
SAAD EL-DIN
1
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
AUTHOR
FATHIYA
GAWISH
2
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, PO Box 30, Imbaba, Giza, Egypt.
AUTHOR
HANAA
ABU EL EININ
3
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, PO Box 30, Imbaba, Giza, Egypt.
AUTHOR
SHEREEN
MANSOUR
4
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, PO Box 30, Imbaba, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
PHARMACOLOGICAL AND ANTIOXIDANT ACTIONS OF GARLIC AND/OR ONION IN NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD) IN RATS
Non-alcoholic fatty liver disease (NAFLD) includes a broad spectrum of fat-induced liver injury, ranging from mild steatosis to cirrhosis and liver failure. This study investigates the hepatoprotective properties of garlic and onion in NAFLD rat model. Ninety male Sprague- Dawley rats were randomly divided into 9 groups; normal (I), NAFLD induced with high fat diet (HFD; II), NAFLD switched to regular diet (RD; III), NAFLD-HFD or NAFLD-RD treated with garlic (IV, V), onion (VI, VII) or the combined garlic+onion (VIII, IX) respectively. A NAFLD rat model was established by feeding the animals with a high-fat diet for 12 wk. These animals were then treated with garlic or/and onion or vehicle for 8 wk (weeks 13-20) and then killed to obtain serum samples and liver tissues. Liver histology, lipids, parameters of oxidative stress, TNF- and TGF- were measured. The liver in NAFLD-HFD showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration. Serum levels of ALT, AST, ALP, leptin, cholesterol, triglycerides, TNF-α, TGF- and hepatic MDA were significantly increased (P<0.05) compared with normal group. This was accompanied with reduction of hepaticGSH, GR, GPx, GST, SOD and serum adiponectin. These changes were to a less degree in NAFLD-RD group. Combined administration of garlic+onion produced a better and significant decrease in liver steatosis, serum liver enzymes, oxidative markers and lipid peroxidation versus each one alone. In the same time, NAFLD-induced inflammation was also mitigated via reduction of TNF- and TGF-. In addition, these results were better in the group IX versus group VIII.
https://jesp.journals.ekb.eg/article_90380_d197496dbf3f4724ff995da12844f10b.pdf
2014-08-01
295
308
10.21608/jesp.2014.90380
Fatty Liver
Garlic
onion, leptin
adiponectin
TGF-
TNF-
liver enzymes and antioxidants
SAYED
SEIF EL-DIN
1
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El- Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
AUTHOR
ABDEL-NASSER
SABRA
2
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El- Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
AUTHOR
OLFAT
HAMMAM
3
Department of Pathology, Theodor Bilharz Research Institute, Warak El- Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
AUTHOR
FATMA
EBEID
4
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El- Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
AUTHOR
NAGLAA
EL-LAKKANY
5
Department of Pharmacology, Theodor Bilharz Research Institute, Warak El- Hadar, Imbaba, P.O. Box 30, Giza 12411, Egypt.
AUTHOR
ORIGINAL_ARTICLE
ANATOMICAL AND SURFACE MORPHOLOGY OF HEDRURIS LUTJANENSES SP. N. (NEMATODA: HEDRURIDAE) FROM THE COMMON MARINEWATER FISH LUTJANUS SYNAGRIS IN DAMIETTA, EGYPT
The description and tegumental ultrastructure of nematode Hedruris lutjanenses sp. n. collected from marine Lane Snapper fish Lutjanus synagris Linnaeus, 1758 collected at Manzala Lake, Damietta, Egypt was studied by light and Scanning Electron Microscopy (SEM). The new species is mainly characterized by that the buccal capsule is surrounded by four pseudolapia; one small and three larger pseudolapia, each one bears a number of papillae arranged irregularly on its surface and the presence of one pair of precloacal papillae and nine pairs of postcloacal ones in males.
https://jesp.journals.ekb.eg/article_90381_61a5e02155ab343e279207fdc3cc029b.pdf
2014-08-01
309
320
10.21608/jesp.2014.90381
Egypt
Damietta
Hedruris Lutjanenses
Sp. N. Lutjanus synagris
M.
RAMADAN,
1
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
AUTHOR
E.
AWAD
2
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
AUTHOR
R.
TAHA
3
Department of Biology, Faculty of Education, Ain Shams University, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
MOLECULAR CLONING AND EXPRESSION OF THE LEISHMANIA TROPICA KMP-11 GENE
Kinetoplastid membrane protein-11 (KMP-11) is a small protein of 11 kDa present in all kinetoplastid protozoa studded so far. This protein which is highly expressed in all stages of the Leishmania life cycle is considered a potential candidate for a leishmaniasis vaccine against many leishmania species. KMP-11 has been recently described in Leishmania tropica.In the present study, the KMP-11 gene was extracted from L. tropica by PCR using two oligonucleotide primers designed to amplify the entire coding region of this gene. Then, the purified PCR products were successfully ligated into a high expression vector the pRSETGFP. This expression vector provides the opportunity to clone the desired insert as a fusionprotein with a GFP and a tag, polyhistidine region. The GFP use as a carrier to improve immune response and the polyhistidine tag facilitates detection of the expressed protein with anti-His antibodies and also purification of the protein using affinity purification. After wards KMP-11 coding region was sequenced and the recombinant protein was induced andpurified from Escherichia coli cultures. The results of the present study will increase our knowledge about molecular cloning and expression of the L. tropica KMP-11 gene, and this may be used as an effective target for controlling cutenous leishmaniasis.
https://jesp.journals.ekb.eg/article_90383_0ff495e321e9cc145fa75f1d8930a199.pdf
2014-08-01
321
328
10.21608/jesp.2014.90383
Leishmania tropica
Molecular cloning
expression
MOUAYAD
MERIEE
1
Department of Animal Biology, Faculty of Sciences, Damascus University, Syria.
AUTHOR
CHADI
SOUKKARIEH
2
Department of Animal Biology, Faculty of Sciences, Damascus University, Syria.
AUTHOR
ABDUL QADER
ABBADY
3
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), Damascus, Syria.
AUTHOR
ORIGINAL_ARTICLE
SCREENING OF TOXOPLASMA GONDII INFECTION AMONG CHILDBEARING AGE FEMALES AND ASSESSMENT OF NURSES’ ROLE IN PREVENTION AND CONTROL OF TOXOPLASMOSIS
Toxoplasmosis, caused by Toxoplasma gondii is an obligate intracellular zoonotic protozoan parasite, with a worldwide distribution particularly in Arab countries including Egypt. The study evaluated toxoplasmosis infection among childbearing age Egyptian females and assessed the military nursing staff knowledge, attitude and compliance to toxoplasmosis prevention and control measures. The study was conducted in a general military hospital. Research design: cross-section descriptive research design was used to conduct this study. The subjects consisted of 14 young females (11 were in-patients undergoing gynecological treatment in a military hospital and 3 were staff nurses. On the other hand, 44 staff nurses were available for assessment who met the inclusion criteria Tools: 4 tools were used for data collection: first consisted of self-administered questionnaires to assess nurses’ socio-demographic data and knowledge, second rating scale to assess nurses’ attitude towards toxoplasmosis infection and its prevention, third performance check list tomeasure nurses’ compliance to infection control measures, and fourth measured the anti- Toxoplasma antibodies by commercial indirect hemagglutination test (IHAT). The results showed that almost half of the nurses had satisfactory levels of knowledge, attitude, and compliance to toxoplasmosis infection control measures. 22.2% of the pregnant womenand 20% of non-pregnant ones showed antibodies against T. gondii. Thus health education about toxoplasmosis should be tailored to women whether married or single to help in avoiding the risk of infection. Frequent periodic IHAT should be done for people who continuously contact with cats. Adherence to strict infection prevention measures is a must to eliminate exposure to toxoplasmosis infection. Training intervention should be implemented to achieve successful improvement in knowledge, attitude, and compliance of toxoplasmosis control measures.
https://jesp.journals.ekb.eg/article_90384_95ec7efbdb199423282dd12095539701.pdf
2014-08-01
329
342
10.21608/jesp.2014.90384
Toxoplasmosis
Knowledge
Attitude
Compliance
Nursing Staff
Military hospital
AHMED
SALEH
1
Military Institute of Health and Epidemiology.+
AUTHOR
HISHAM
ALI
2
The Military Medical Academy, Egypt.
AUTHOR
SALWA
AHMED
3
Military Institute of Health and Epidemiology and Medical Surgical Nursing, The Military Medical Academy, Egypt.
AUTHOR
SAMAH
HOSNY
4
Military Institute of Health and Epidemiology, The Military Medical Academy, Egypt.
AUTHOR
TOSSON
MORSY
5
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
ORIGINAL_ARTICLE
INITIAL EXPERIENCE OF SURGICAL MICROWAVE TISSUE PRECOAGULATION IN LIVER RESECTION FOR HEPATOCELLULAR CARCINOMA IN CIRRHOTIC LIVER
Surgical hepatic resection has been considered as the first-line treatment which is most effective and radical treatment for HCC, however, HCC is usually associated with poor liver function owing to chronic hepatitis or liver cirrhosis. Techniques that can eradicate the tumor and also preserve liver function are needed. Moreover, hepatic resection, in the presence of cirrhosis, raises special problem of high risk as hemorrhage and liver failure, thus, good clinical results can only be achieved by minimizing operative blood loss, time of the intervention as well as the hepatic reserve. The tremendous progress in microwave technology has recently attracted considerable attention. This study evaluated the feasibility of this new liver transection technique demonstrating the high performance of this procedure, the accuracy in terms of squeeze effect on veins and portal branch and in terms of reducing the intra operative blood loss, and minimizing the operative time for safe hepatectomy. Twenty-six consecutive patients a first-time diagnosis of hepatocellular carcinoma (HCC) ontop of liver cirrhosis were recruited for the study, from August 2011 to January 2013.All patients were subjected to full clinical examination, laboratory investigations, abdomen ultrasound (U/S), triphasic computed tomographic liver scan (CT) and dynamic magnetic resonance imaging (MRI) in some doubtful cases. Inclusion requirements were presence of resectable disease without vascular invasion or extrahepatic spread at imaging, Child-Pugh class A & B (Score 7) liver cirrhosis, (INR) < 1.6or platelet count ›60 000/mm3 with no previous treatment. Patients were treated by applying pre-coagulation of the liver transection lines using microwave probe positioned in parallel to the line of resection by open approach after intraoperative U/S assessment for localization of the tumor and line of resection. The procedures were performed under general anesthesia. Mobilization of the liver was not necessary to be done in all cases. Intra-operative ultrasound (Aloka, Tokyo, Japan) was used to exclude lesions not detected pre-operatively (operative surprise), to define the location, number and size of the tumor and to identify large intra-hepatic large vascular and biliary structures,and guide insertion of the microwave applicator. Intraoperative ultrasound was used again, to assess that the line of resection was done. Primary endpoints documented were total operative time, time for liver parenchyma transection, intraoperative blood loss and blood transfusion requirements. Secondary endpoints included postoperative complications, mortality and intensive care unit (ICU) together with hospital stay.
https://jesp.journals.ekb.eg/article_90385_4013aef3d8a0241dabe8eb997f5c4c58.pdf
2014-08-01
343
350
10.21608/jesp.2014.90385
microwave
Hepatectomy - liver - Cirrhosis - Hepatocellular carcinoma
A.
ABDELRAOUF
1
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
AUTHOR
H.
HAMDY
2
Department of General Surgery, Theodor Bilharz Research Institute, Giza 12411, Egypt.
AUTHOR
A.
EL ERIAN
3
National Institute of Endocrinology, National Institute of Diabetes & Endocrinology, Cairo, Egypt.
AUTHOR
M.
ELSEBAE
4
Department of General Surgery, Theodor Bilharz Research Institute, Giza 12411, Egypt.
AUTHOR
S.
TAHA
5
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egpt.
AUTHOR
H.
ELSHAFEY
6
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
AUTHOR
S.
ISMAIL
7
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
AUTHOR
M.
HASSANY
8
Department of Hepato-Bilio-Pancreatic Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
A DESCRIPTION OF HAEMOGREGARINA SPECIES NATURALLY INFECTING WHITE-SPOTTED GECKO (TARENTOLA ANNULARIS) IN QENA, EGYPT
The present study describes the developmental stages of Haemogregarina species in the blood and tissues of naturally infecting white-spotted gecko Tarentola annularis collected from Qena, Egypt. Different parasite’s forms were observed infecting the erythrocytes. The gamonts enclosed within parasitophorous vacuole and seems to have no clear effect on thehost cell especially in case of immature parasite forms. But in the presence of mature gamonts the host cell nucleus displaced. The parasitaemia level is up to 280 per 10,000 erythrocytes counted. Trophozoites and gamonts have been recognized in the blood smears. The rounded trophozoite diameter is 3.84±0.87 μm, while the elongated trophozoite measured 4.42±0.69×2.8±0.56 μm. The mature gamonts were differentiated into two forms; short gamont measuring 10.82±0.82×3.30±0.73 μm (range: 10-12.1×2.2-4.4 μm) and the long gamont measured 14.67±0.83×3.96±0.77 μm (range: 14.1-16.5×3.3-5.5 μm). Merogony carried out only in the endothelial cells of the blood capillaries in the lung. Different merogonic stages have been recognized and differentiated in two forms; micromeront measured 13.250.50×120.0 μm and produces a few number of large merozoites, macromeront measured 19.750.87×13.250.50 μm and produces more small sized merozoites. The gamonts and merozoites have the general characteristic ultrastructures of the Apicomplexa containing components of the apical complex, e.g. pellicle, micronemes, rhoptries, and few dense bodies and subpellicular microtubules.
https://jesp.journals.ekb.eg/article_90387_c9e85d0c5d6822dadf45de76d0eb8560.pdf
2014-08-01
351
359
10.21608/jesp.2014.90387
Haemogregarina
Tarentola annularis
Parasitaemia
light and electron microscope
Egypt
SOHEIR
RABIE
1
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt.
AUTHOR
ABDEL-NASSER
HUSSEIN
2
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt.
AUTHOR
ORIGINAL_ARTICLE
HYDROCARBON INSECTICIDES: THEIR RISKS FOR ENVIRONMENT AND HUMAN HEALTH
Insecticides are used to control diseases spread by arthropods, but theys vary greatly in toxicity. Toxicity depends on the chemical and physical properties of a substance, and may be defined as the quality of being poisonous or harmful to animals or plants. Poisons have many different modes of action, but in general cause biochemical changes which interferewith normal body functions. Toxicity can be either acute or chronic. Acute toxicity is the ability of a substance to cause harmful effects which develop rapidly following absorption, i.e. a few hours or a day. Chronic toxicity is the ability of a substance to cause adverse health effects resulting from long-term exposure to a substance. There is a great range in the toxicity of insecticides to humans. The relative hazard of an insecticide is dependent upon the toxicity of the pesticide, the dose received and the length of time exposed. A hazard can be defined as a source of danger. The great majority of insecticides are poisonous to man and his beneficial insects and animals and are carcinogenic agents particularly, the halogenated hydrocarbons containing benzene ring.
https://jesp.journals.ekb.eg/article_90389_8d95ab6b521408de6189921ae5a3b2ab.pdf
2014-08-01
361
372
10.21608/jesp.2014.90389
Halogenated hydrocarbons
environmental pollution
Human health
MAMDOUH
EL-BAHNASAWY
1
Military Medical Academy, Egypt.
AUTHOR
AMINA
MOHAMMAD
2
Military Medical Academy, Egypt.
AUTHOR
TOSSON
MORSY
3
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
ORIGINAL_ARTICLE
DESCRIPTION OF TWO NEW CERCARIAE (AN ECHINOSTOME CERCARIA AND A XIPHIDIOCERCARIA) PROCURED FROM BIOMPHALARIA PFEIFFERI (KRAUSS) FROM NIGERIA
During parasitological examination of Biomphalaria pfeifferi snails obtained from Niger state (Nigeria), 2 new types of cercariae were found. They are identified to the level of referring to the major group and described here for the first time. They were examined viable and stained with vital stains as well as fixed in 70% alcohol. They were drawn with a camera lucida and photographed. They are identified as an echinostome cercaria and a xiphidiocercaria. The echinostome is characterized by having a ventral sucker almost double in size the oral one. It has a semicircular structure located beyond the oral sucker. Three pairs of penetration glands are found at the anterior portion of the body. The number of collar spines is relatively large (44-46). The flame cells are 17x2 in number. Two main lateral excretory ducts extend anteriorly,form two typical echinostome loops then pass posteriorly to open together in a diverticulated excretory vesicle. Its tail is relatively long and flattened with 3 fin folds. The tail (640 μm) is longer than the body (475μm). The xiphidiocercaria belongs to the "ornatae" group. It is relatively small (180.5x110μm) with a long stylet (30μm). Its oral sucker is one and half times the size of the ventral sucker. Two excretory ducts extend posteriorly in both sides and become dilated and unite to open in a circular excretory vesicle. Tail is slender shorter than the body and has a dorso-ventral fin fold.
https://jesp.journals.ekb.eg/article_90391_d0bcbd30d2cc2e3e3af1f73848a78ea0.pdf
2014-08-01
373
380
10.21608/jesp.2014.90391
Echinostome cercaria
xixphidiocercaria
Biomphalaria pfeifferi
Nigeria
FOUAD
YOUSIF
1
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, Imbaba, Egypt.
AUTHOR
MAGDA
AYOUB
2
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, Imbaba, Egypt.
AUTHOR
MENERVA
TADROS
3
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, Imbaba, Egypt.
AUTHOR
SAMIA
EL BARDICY
4
Department of Environmental Research and Medical Malacology, Theodor Bilharz Research Institute, Imbaba, Egypt.
AUTHOR
SIMON
ABOLARINWA
5
Department of Biological Sciences, Federal University of Technology. Minna, Niger State, Nigeria.
AUTHOR
ORIGINAL_ARTICLE
BRONCHIAL ASPIRATES GLUCOSE LEVEL AS INDICATOR FOR METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN INTUBATED MECHANICALLY VENTILATED PATIENTS
This study evaluated if the level of glucose in bronchial aspirate serves as indicator for the risk of MRSA infection in intubated mechanically ventilated ICU patients. A total of 50 critically ill patients was enrolled and were under tight glycemic control to abolish the effect of hyperglycemia on bronchial secretion, if they were expected to require mechanicalventilation for more than 48 hours. Bronchial aspirates were detected for glucose and sent twice weekly for microbiological analysis and whenever an MRSA was expected. The results showed that all the patients had glucose tested in bronchial aspirates. Glucose was detected in bronchial aspirates of 28 of the 50 patients. Glucose in bronchial aspirates in thesepatients ranged between (2.9-5.1mmol/l). MRSA was detected in 22 patients where 28 were MRSA free of the MRSA patients 19 had positive glucose where glucose was positive in 28 patients of them 19 (86.4%)where MRSA positive to 9 with no MRSA (32.1%).The risk of having MRSA present markedly increased significantly in the presence of glucose: (p value .001)
https://jesp.journals.ekb.eg/article_90392_478f4d043eb9cda0fc2f9e14ab2a3ef3.pdf
2014-08-01
381
388
10.21608/jesp.2014.90392
Glucose level
Indicator
Methicillin-resistant Staphylococcus aureus
Ventilated patients
SHERIF
ALSAYED
1
Department of Chest, Faculty of Medicine, El Fayoum University, Egypt.
AUTHOR
SAMAR
MARZOUK
2
Department of Biochemistry, Faculty of Medicine, Cairo University, Egypt.
AUTHOR
ESSAM
MOUSA
3
Department of Internal Medicine, Faculty of Medicine, Al Azhar University, Egypt.
AUTHOR
ASHRAF
RAGAB
4
Department of Anesthesia, Faculty of Medicine, Cairo University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
PRELIMINARY STUDY ON CRYPTOSPORIDIOSIS IN LIVESTOCK FROM KUWAIT
Out of 225 fecal samples (40 of calves, 128 of lambs and 57 of goat kids) received in the Veterinary Laboratories, Kuwait, 43 (19.1%) were found to be positive for Cryptosporidium using the conventional method, modified acid-fast stain, and the immunologic technique, the immuno-chromatography assay. Calf fecal samples showed the highest infection rate (37.5%),followed by goat kids (21%) and then Lambs (12.5%). Results obtained by conventional method were found to be consistent with those detected using the immunologic technique.
https://jesp.journals.ekb.eg/article_90393_24bc860f84bf136d9939a9f69c46b9cc.pdf
2014-08-01
389
392
10.21608/jesp.2014.90393
cryptosporidiosis
livestock
Diagnosis microscopic
Immunology
QAIS
MAJEED
1
College of Basic Education, The Public authority for Applied Education and Training, Kuwait.
AUTHOR
MAHA
ALAZEMI
2
Department of Criminal Evidences, Ministry of Interior, Kuwait.
AUTHOR
ORIGINAL_ARTICLE
PREVALENCE OF ANTI-TOXOPLASMA GONDII ANTIBODIES IN ABORTED EWES IN KUWAIT
Serum samples from 528 aborted ewes from different sheep flocks in Kuwait were tested for Toxoplasma gondii. The indirect hemagglutination test (IHAT) was used with a titer of 1:80 considered positive. Antibodies to T. gondii were found in 94 (17.8%). The highest number of positive ewes (23; 24.5%) had a titer of 1:640. Only one aborted ewe had a titer of1:2560. This study is the first to report Toxoplasma infection in sheep which may pose risk to humans who consume under cooked mutton. The detection of Toxoplasma antibodies in aborted ewes refers to the implication of toxoplasmosis in abortion among sheep in Kuwait.
https://jesp.journals.ekb.eg/article_90394_cbc12dcf39f983a081dc9ba8bb17af48.pdf
2014-08-01
393
396
10.21608/jesp.2014.90394
Kuwait
Toxoplasmosis
sheep
Abortion
IHAT
MAHA
ALAZEMI
1
Department of Criminal Evidences, Ministry of Interior, Kuwait.
AUTHOR
ORIGINAL_ARTICLE
PREVALENCE OF SCHISTOSOMIASIS IN AL-BAHA PROVINCE, SAUDI ARABIA IN YEARS 2012 AND 2013 (PROSPECTIVE AND COMPARATIVE STUDY)
The rate of human schistosomiasis increased in Al- Baha Province, Saudi Arabia in the last few years. So, it was necessary to study the prevalence of human schistosomiasis in Al- Baha Province over the period of the two years (2012-2013).In general, there was a decline in the prevalence of schistosomiasis in Al- Baha over the study period and it was significantly less in 2013 than it was in 2012. Schistosomiasis infection rate was affected by host sex as males had higher infection rate than females. Age group of 15-44 showed the highest infection rate. Prevalence of schistosomiasis in Saudipeople was significantly higher than it was in non-Saudi or immigrant ones. Thus, more attention should be paid to Al-Baha area in the future schistosome control programmes.
https://jesp.journals.ekb.eg/article_90395_79a28fd05a5981e3fb206a5414b13b4f.pdf
2014-08-01
397
404
10.21608/jesp.2014.90395
Saudi Arabia
Al-Baha
Schistosomiasis
Prospective study
KHAIRY
MOHAMMAD
1
Departments of Parasitology and Faculties of Medicine, Al-Azhar University, Cairo, Egypt and Al-Baha University, Al-Baha, Saudi Arabia.
AUTHOR
ORIGINAL_ARTICLE
AN INTERVENTIONAL PROGRAM FOR NURSING STAFF ON SELECTED MASS GATHERING INFECTIOUS DISEASES AT HAJJ
This work improved military nursing staff knowledge on selected mass gathering infectious diseases at Hajj. The results showed that only (20%) of the participating nurses attended training program about health hazard during pilgrim. But only (40.0%) of them found the training programs were specific to nurses. Majority found the program useful (70.0%), and the average duration of this training program in weeks was 3.5+1.1. There was significant improvement P=<0.001, of correct knowledge about meningitis regarding causes, organisms, mode of spread, people at risk, transmission,prevention and treatment, the highest improvement was in causes of meningitis the lowest was in adult vaccination. 25% of participants had adequate knowledge (>60% from total score) in pre-test 93% in post-test 72% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement of correct knowledge P=<0.001 about seasonal influenza and respiratory diseases during pilgrim, the highest improvement was in influenza vaccine strains the lowest was in antiviral drugs. 23% of nurses had adequate knowledge (>60% from total score) in pre-test 94% in post-test 66% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement P=<0.001 of correct knowledge about gastrointestinal diseases and food poisoning during pilgrim among nurses at military hospital, the highest improvement was in risk factors of food poisoning the lowest was in what GE patient should do. 22% of participants had adequate knowledge (>60% from total score) in pretest 91% in post-test 58% after 3 month with significant difference among tests regarding adequate knowledge. There was significant improvement P=<0.001 of correct knowledge about heat exhaustion during pilgrim among nurses at military hospital, the highest improvement was in non-communicable diseases the lowest was in sun stroke prevention. 27% of participant hadadequate knowledge (>60% from total score) in the pre-test 94% in the post-test 74% after 3 month with significant difference among pre, post and FU regarding adequate knowledge. Also, there were significant improvement P=<0.001 of correct knowledge about hypertension, dengue fever, skin scalding & others diseases during pilgrim among nurses at military hospital, the highest improvement was in skin scalding prevention the lowest was in first aid bag. 28% of participant had adequate knowledge (>60% from total score) in the pre-test 92% in the post-test 61% after 3 month with significant difference among pre, post and FU regarding adequate knowledge. There was a significant difference between total knowledge score according to education, and work experience (P>0.05) in the pre, post and after 3 month in age and in all intervention time in department the highest was ICU then ward then operation room.
https://jesp.journals.ekb.eg/article_90396_48fd1d0b655ce8f94ceb7e3301688b84.pdf
2014-08-01
405
424
10.21608/jesp.2014.90396
Egypt
Military Nursing Staff
Mass gathering infectious diseases
pilgrimage
MAMDOUH
EL-BAHNASAWY
1
Consultant of Endemic Diseases and Fevers, Military Medical Academy, Egypt.
AUTHOR
NAGWA
ELMENIAWY
2
Military Medical Institute, Maadi Military Hospital, Egypt.
AUTHOR
TOSSON
MORSY
3
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
ORIGINAL_ARTICLE
NATURAL AND EXPERIMENTAL EVIDENCE OF VISCEROTROPIC INFECTION CAUSED BY LEISHMANIA TROPICA FROM NORTH SINAI, EGYPT
Cutaneous leishmaniasis (CL) is a neglected clinical form that is quite prevalent in Eastern North parts of the country in Sinai Peninsula. Leishmania tropica was identified by previous reports as the causative agent responsible for viscerotropic infections in patients and experimental animals. Here, we reported the viscerotropic infections from naturally infected rodent Gerbillus pyramidum floweri collected from North-Sinai. Footpad and tail lesions, spleenomegaly, and malformed dark-colored spleen were the characteristic CL symptoms. The spleen of the rodent found positive to amastigote impression smear. ITS-1 DNA was sequenced and revealed 100% identity of the strain in the current study to the other L. tropica sequences identified from the patients with the suspected CL and inhabited the same study area. The current findings confirmed the susceptibility of gerbil to L. tropica, and raise the concerns for the role of rodents as accidental host suffering the infections. The susceptibility of wild and experimental rodents to the same L. tropica strain was also investigated; BALB/c and G. pyramidum were more susceptible to L. tropica (24.33±4.37 and 25±4.58 days post-infection, respectively). Similar viscerotropic pathologies were reported in experimental infection of only golden hamster (≈ 120 days post-infection), and G. p. floweri (≈ 160 days post-infection).
https://jesp.journals.ekb.eg/article_90398_3d6861d3eec113291459811d632d2ff4.pdf
2014-08-01
425
434
10.21608/jesp.2014.90398
Viscerotropic
L. tropica
North Sinai
ITS-1 DNA
Splenomegaly
SAID
DOHA
1
Research and Training Center on vectors of diseases, Faculty of Science building, Ain Shams University, Egypt.
AUTHOR
MAGDI
SHEHATA
2
Entomology Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
ADEL
FAHMY
3
Entomology Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
ABDALLAH
SAMY
4
Entomology Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.+
AUTHOR
ORIGINAL_ARTICLE
PFEIFFERINELLA SP. (PFEIFFERINELLIDAE, APICOMPLEXA) INFECTING THE FRESH WATER SNAIL PIRENELLA CONICA LIGHT AND ELECTRON MICROSCOPE STUDIES
Coccidian oocysts were proved to be found in 70 of 100 collected Pirenella conica snails, with a natural infection of 70%. It was observed that, Pfeifferinella sp. was transferred between hepatopancreas and small intestine of snail. The prepatent period of Pfeifferinella sp. infecting P. conica snails ranged from 14-18 days and the patent period was reached 50 days (P.I.). Merogony stages were the early stages observed in this study. These stages were observed in the hepatopancreas and in a large clear parasiteophorous vacuole (PV). In snails killed 4 days P.I. immature meronts were measured 12 х 10 μm containing 8 nuclei. Meanwhile, mature meronts with about 6 differentiated merozoites were detected as early as 6 days P.I., and measured 3.1х1.4μm. The earliest gametogonic stages were seen in the intestine of Pirenella conica snailskilled 12 days P.I. Microgamonts contained about 4 nuclei and measured 7.9х6.7μm. The macrogamonts measured 7.3х5.6μm. Macrogametes were characterized by the presence of the vaginal tube, this tube measured 4.3х1.1μm. Fertilization was occurred in the intestine of the infected snails at 12 days P.I. Zygotes developed into young oocysts after fertilization. Sporogony occurred in the intestine. In the earliest stage, the nucleus of young oocyst was occupied the central position that were observed through the examination of the intestine of infected Pirenella snails at 14 day P.I. These oocysts were found to be colorless and ellipsoid or spherical in shape measured 9.5 х 8.5μm. The oocyst wall consists of two layers, micropyle and micropyle cap were not observed in these oocysts, and wall forming bodies were arranged at the periphery of oocyst directly under the developed oocyst wall. Sporulated oocyst contains 8 sporozoitesfilling the entire cavity of the oocyst without sporocyst formation. Fully sporulated oocysts were excreted in the faces of infected snails from 14-18 day P.I., these oocysts measured 9.5х8.5μm. Micropyle was absent and while a residual body was observed.
https://jesp.journals.ekb.eg/article_90399_0ca8f97ddb427fb341fe30c41683af52.pdf
2014-08-01
435
446
10.21608/jesp.2014.90399
Egypt
Beni Suef Governorate
Pfeifferinella sp
Pirenella conica
Morphology
HODA
EL-FAYOMI
1
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
AUTHOR
HAYAM
MOHAMMED
2
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
AUTHOR
THABET
SAKRAN
3
Department of Zoology, Faculty of Science, Beni Suef University, Beni Suef, Egypt.
AUTHOR
ORIGINAL_ARTICLE
THE EFFECT OF LARVAL AND ADULT NUTRITION ON SURVIVAL AND FECUNDITY OF DENGUE VECTOR AEDES ALBOPICTUS SKUS (DIPTERA: CULICIDAE)
The effect of larval and adult nutrition on survival and fecundity of the dengue vector Aedes albopictus Skuse (Diptera: Culicidae) were studied under laboratory conditions, the energy for the physiological activities for both male and female mosquitoes is provided largely by their eserves during larval stage and affected by adult diets. Two groups of larvae (A, B) were reared at 27±3oC, 70-80% R.H. and DL. 12: 12. Group (A) with 200 larvae (high larval diet) and Group (B) with 600 larvae (low larval diet). Ae. albopictus exhibited increased fecundity and egg hatch success. Immature development was quick. Immature survival was high, with lowest rate in the pupal stage. Highest longevity was observed in large females fed water + 10% sucrose solution (29.571±0.415 days) while the lowest one was (1.3±0.132 days) in starved small females. Large females have significantly (P< 0.001) higher fecundity than smaller females, regardless of whether the females were provided 10% sucrose solution or not (524±0.203 eggs/group 159.714±0.1997 eggs/group), respectively. The addition of 10% sucrose solution significantly (P< 0.001) increase the fecundity regardless of whether large orsmall females (657.9±0.2198 eggs/group, 242.429±0.119 eggs/group), respectively.
https://jesp.journals.ekb.eg/article_90400_4559c4edaaaceddb36554478f5faed88.pdf
2014-08-01
447
454
10.21608/jesp.2014.90400
nutrition
longevity
fecundity
Aedes albopictus
ABEER
YAMANY
1
Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt.
AUTHOR
FATMA
ADHAM
2
Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
UPDATE IN DIAGNOSIS AND TREATMENT OF CRYPTOSPORIDIOSIS AN UPDATE REVIEW
Cryptosporidiosis represents a major public health problem which transmitted by contamination of food or water by sporulated Cryptosporidial oocyst. Causing self- limited diarrhea in immunocompetent person and chronic and life threatening diarrhea among immunocompromised individuals. It can be diagnosed by concentration and detection of its Oocyst in different environmental samples and water by microscopic and immunological examination such as enzyme immunoassay (ELISA) for parasite antigens and nucleic acid amplification assay as well as use of molecular techniques such as Polymerase Chain Reaction (PCR). Treatment is face challenges, Macrolides, Paramomycin, Nitazoxanide and Mirazid. All these drugs have partial efficacy in reducing disease severity in immunocompetent individuals. Nitazoxanide has partial efficacy in immunocompromised individuals. Resolution of Cryptosporidiosis can be maintained with effective Highly Active Antiretroviral Therapy (HAART).
https://jesp.journals.ekb.eg/article_90402_a23e4e92b329d64010e03fb830dd2be0.pdf
2014-08-01
455
466
10.21608/jesp.2014.90402
Cryptosporidosis
C. parvum
C. hominis
diarrhea
Diagnosis
Treatment
OSAMA
ABDALLA
1
Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, Egypt.
AUTHOR
ORIGINAL_ARTICLE
UROTHELIAL CARCINOMA OF THE URINARY BLADDER MIXED WITH SQUAMOUS DIFFERENTIATION OR SQUAMOUS CELL CARCINOMA IN AREAS WITH SCHISTOSOMIASIS IS SHOWING HIGH RISK OF RECURRENCE AND POOR SURVIVAL
In schistosomiasis haematobium areas endemic, bladder cancer is the first cause of malignancy in men and fourth in women. The chronic schistosomiasis would lead to variant histologic patterns which manifest in squamous cell carcinoma (SCC) or squamous differentiation (SqD). This study evaluated the clinical outcome after radical cystectomy (RC) in patients with urothelial carcinoma (UC) mixed with SCC or SqD, Comparison was done with two arms of pure UC and pure SCC, indication for RC was muscle-invasive-disease, and evaluation included recurrence, metastases, and overall survival. The data of patients treated with RC for muscleinvasive-disease, selection was revised for 127 patients with urothelial carcinoma mixed with SCC/SqD, two comparative arms were 100 patients with pure UC, and 100 patients had pureSCC. Follow up was on 8 months, 3years, and 5 years to detect recurrence, metastasis, and overall survival in the three groupsThe results showed that by comparison of disease aggressiveness in the three groups regardingrecurrence, metastasis, and overall survival was analysed. Overall survival with mixed tumourswas significantly lower than pure UC or SCC, recurrence and metastases were higher in mixedtumour which was an independent factor for poor prognosis and low survival.
https://jesp.journals.ekb.eg/article_90403_ac3dee4b03ccd4b4939f93a11a092266.pdf
2014-08-01
467
473
10.21608/jesp.2014.90403
Egypt
Schistosomiasis
Bladder cancer
Urothelial carcinoma
Squamous cell carcinoma
differentiation
Mixed tumour
prognosis
MOHAMED
WISHAHI
1
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba, Giza, POB 30, Egypt.
AUTHOR
HOSSAM
ELGANZOURY
2
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba, Giza, POB 30, Egypt.
AUTHOR
AMR
ELKHOULY
3
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba, Giza, POB 30, Egypt.
AUTHOR
MOHAMED
BADAWI
4
Department of Urology, Theodor Bilharz Research Institute, Kornish ELNil, Embaba, Giza, POB 30, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFICACY OF PENTOXIFYLLINE AS AN ANTIFIBROTIC DRUG IN EXPERIMENTAL MURINE SCHISTOSOMAL HEPATIC FIBROSIS
This study evaluated the possible antifibrotic effect of pentoxifylline on experimentally induced schistosomal hepatic fibrosis and its effect on serum leptin and transforming growth factor- β1 levels as possible antifibrotic mechanisms in correlation with the hepatic fibrosis indices. A total of ninety clean laboratories bred, males Swiss, albino mice were included, of which ten mice served as a control non-infected, non-treated group and sacrificed at one time. Eighty mice, each was subcutaneously infected with 50 Schistosoma mansoni cercariae and classified into groups: GI (infected & non-treated), GII (infected & treated with Mirazid®), GIII (infected & treated with Pentoxifylline®) and GIV (infected & treated with a combination of Mirazid and Pentoxifylline). Each group was further subdivided into 2 subgroups; subgroup ‘a’ which started treatment at 6th week post-infection (P.I.) and sacrificed at the end of 9th week P.I and subgroup ‘b’ which started treatment at 14th week P.I and sacrificed at the end of 17th week P.I. The efficacy of the treatment was assessed by histopathological examination of the liver with measurement of granuloma sizes, estimation of hydroxyproline content in the liver, and assessment of serum levels of leptin and transforming growth factor- ß1 (TGF- ß1) Mirazid (MZD) caused significant reductions in granuloma sizes and hepatic hydroxyproline content and caused non-significant reductions in serum levels of leptin and transforming growth factor- ß1 at 9th & 17th weeks P.I (GII). Pentoxifylline (PTX) caused significant reductions in granuloma sizes, hepatic hydroxyproline, and serum levels of leptin and transforming growthfactor- ß1 at the 9th & 17th weeks P.I (GIII). While combined therapy of both MZD & PTX in GIV caused more reductions in granuloma sizes, hepatic hydroxyproline, and serum levels of leptin and TGF- ß1 at the 9th & 17th weeks P.I when compared to the other groups.
https://jesp.journals.ekb.eg/article_90404_cb1a726936530f880eddd94ab96ba103.pdf
2014-08-01
475
488
10.21608/jesp.2014.90404
Mice
Schistosomal hepatic fibrosis
Mirazid
Pentoxifylline
Histo-pathology
EMAN
KHALIFA
1
Department of Microbiology, Faculty of Medicine, Taif University, Taif, Kingdom of Saudi Arabia.
AUTHOR
DALAL
NEMENQANI
2
Department of Pathology, Faculty of Medicine, Taif University, Taif, Kingdom of Saudi Arabia.
AUTHOR
ORIGINAL_ARTICLE
PREVALENCE OF SCHISTOSOMIASIS AMONG PATIENTS ATTENDING QENA GENERAL HOSPITAL OVER THE YEARS 2011 AND 2012
Schistosomiasis is a public health problem in Egypt. To detect the prevalence of schistosomiasis in Qena Governorate, 1601 urine and stool samples of patients attending Qena General Hospital were examined of whom 1601 patients had urinary symptoms and 893 patients had dysentery and bloody stool. Sheets were filled out on each patient. Also, a total of 7590 Bulinus truncatus and Biomphalaria alexandrina snails were collected from water bodies in Qena Governorate. Theoverall prevalence of S. haematobium was 13.9%; with maximum among 6-18 year-old age group and higher in males than in females. Risk factors for S. haematobium infection were this age group; particularly males. However, males swim and play in water bodies and women wash cloths and utensils there, and children swimming or playing in canals. The S. haematobium peak of infection was in winter correlated with the highest prevalence in Dandara City, Qena City, Awlad-Amr and El Hijarat. S. mansoni were negative in the examined individuals. The infective rate among snails was 1.82% in B. truncatus and 0.0% among B. alexandrina and M. tuberculata.
https://jesp.journals.ekb.eg/article_90406_798b719d57173f7d76b1e4a007a13d9e.pdf
2014-08-01
489
495
10.21608/jesp.2014.90406
Qena Governorate
Schistosomiasis
Snails intermediate hosts
Parasitologic examination
FATMA
SAYED
1
Departments of Parasitology, Faculty of Medicine, Assuit University, Egypt.
AUTHOR
MOHAMMED
MOHAMMED
2
Departments of Parasitology, Faculty of Medicine, Assuit University, Egypt.
AUTHOR
MOHAMMED
ABD-EL-KADER
3
Departments of Parasitology, Faculty of Medicine, Assuit University, Egypt.
AUTHOR
OSAMA
ABDALLA
4
Departments of Parasitology, Faculty of Medicine, Assuit University, Egypt.
AUTHOR
SAMAR
AHMED
5
Community Medicine, Faculty of Medicine, South Valley University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
PATIENT SAFETY ATTITUDE AMONG HEALTH CARE PROVIDERS IN FAMILY HEALTH CARE CENTERS IN CAIRO GOVERNORATE
Two hundred and eighty Health care; physicians, nurses and technicians were included in the study, 133 (47.5) from 6th discrete HCF, 54 (19.3%) from Duwaiqa and 93 (33.2%) from Al Haggana. Ages ranged between 22 and 59 years (35.14±10.13), years of experience in the study group ranged between 1 and 35 years (8.72±8.18) and years of work in group ranged between 1 & 40 years (10.43±8.33). Doctors and nurses were males (72.55%) & females (86%) respectively while technicians were mostly males (60%). The teamwork climate score was 3.98±0.87, 64.0% answered high or very high, 16.4% answered inadequate and 18% had answered few or very few. Safety climate score was 3.61±0.63, 49.28% had high or very high score, 17.14% answered inadequate and 32.15% had answered few or very few. Job satisfaction score was 3.91±0.80, 32.15% had answered few or very few, 17.14% answered inadequate and49.28% answered high or very high. Stress recognition score was 3.61±0.79, 25% had answered few or very few, 28.6% answered inadequate and 45.7% answered high or very high. Perception of management score was 3.48±0.80, 23.2% had answered few or very few, 17.8% answered inadequate and 57.6% answered high or very high. Working condition score was 3.51±0.84, 46.8% had answered few or very few, 17.1% answered inadequate and 35.7% answered high or very high. A significant difference regarding team work score, safety climate score, perception of management score, working condition score with highest value in doctors and lowest in technicians. On the other hand no significant difference was detected regarding job satisfaction score and stress recognition score. A significant difference regarding team work score, safety climate score and perception of management score with high values among older groups. No significant difference was detected regarding job satisfaction score, stress recognition score and working condition score. Also, asignificant difference regarding team work score and safety climate score with higher value in males. Significant difference regarding job satisfaction score and working condition score was detected with higher value in females. No significant difference was detected regarding stress recognition score and perception of management scores.
https://jesp.journals.ekb.eg/article_90408_47adaabb90c9a9d35054905c995e2a8c.pdf
2014-08-01
497
508
10.21608/jesp.2014.90408
Egypt
Family Health Care Centers. Patient Safety
Health Care Providers
MOHSEN
GADALLAH
1
Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
HASNAA
ABOUSEIF
2
Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
DINA
BOULOS
3
Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
HANAA
ELHARONI
4
Specialist in Family Medicine, Ministry of Health and Population, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
COMPARISON BETWEEN ELISA AND VARIOUS STAINS TECHNIQUES IN LABORATORY DIAGNOSIS OF CRYPTOSPORIDIOSIS
Cryptosporidium spp. is an important parasitic protozoa causing diarrhea which is a severe life-threatening diarrhea especially in immunocompromised hosts. We aimed to evaluate the usefulness of detection of Cryptosporidium spp. copro-antigen from fecal specimens by using enzyme linked immunosorbent assay (ELISA) test and comparing its sensitivity and specificity with some staining methods. The results revealed that Modified Acid-Fast stain is considered better than Giemsa in detecting Cryptosporidium species oocysts in faecal smears as their sensitivity were 67.5% and 53.75% respectively. On contrary, ELISA technique is considered the best method used for detection of cryptosporidial infection as its sensitivity is 90%.
https://jesp.journals.ekb.eg/article_90410_7d98c9c3481ca303b6573d849dbc7ea5.pdf
2014-08-01
509
516
10.21608/jesp.2014.90410
Egypt
Cryptosporidium parvum
persistent diarrhea and ELISA technique
NABIL
GABR
1
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519, Egypt.
AUTHOR
MANAL
ABDELLATIF
2
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519, Egypt.
AUTHOR
EKHLAS
ABD EL-HAFEEZ
3
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519, Egypt.
AUTHOR
REHAM
ABD RABOU
4
Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519, Egypt.
AUTHOR
ORIGINAL_ARTICLE
PREVALENCE OF INTESTINAL PARASITES AND ITS IMPACT ON NUTRITIONAL STATUS AMONG PRESCHOOL CHILDREN LIVING IN DAMANHUR CITY, EL-BEHERA GOVERNORATE, EGYPT
This cross sectional study was done in Damanhur City, the Capital of El-Behera Governorate to detect the prevalence of intestinal parasitic infection among preschool aged children and to find out its effect on their nutritional status. Five-hundreds children aged between 2-6 years were enrolled; a detailed questionnaire, complete clinical and anthropometric assessment as well as complete stool analysis and blood picture were done. The study revealed that 51.8% preschool children were infected; Cysts of Entamoeba histolytica and Giardia lamblia were found in 16.8%, and 14.8% respectively. In 1.8% of cases, both cysts were found together. Ova of Ascaris lumbricoides, Enterobius vermicularis, Ancylostoma duodenale and Hymenolepis nana were detected in 14%, 3.4%, 5% & 0.2% of cases, respectively. In 0.4% of cases, ova of A. lumbricoides and E. vermicularis were found together. Mixed infections were found in 3.6% of children. Significantly lower weight for age z-score (WAZ) and weight for height z-score (WHZ) were found among infested children compared to non-infested ones (P<0.05). Moreover, stunting was found in 44.1%, underweight in 39.1% and wasting in 11.5% of infested children. Prevalence of anemia among all studied population was 39%; 48.6% in infested group compared to 28.8% in non-infested children (x=20, P<0.001). Improper hand washing, and playing in the street bare footed, together with playing with animals and family history of parasitic infestation were considered the independent predictors of parasitic infestation by using binary logistic regression.
https://jesp.journals.ekb.eg/article_90412_3d01d5849a57a41ebe324d44fb3df7e1.pdf
2014-08-01
517
524
10.21608/jesp.2014.90412
Egypt
Preschool-children
Intestinal parasites
nutrition
anthropometric measurements
ADHAM
HEGAZY
1
Departments of Pediatric, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
NEVEEN
YOUNIS
2
Departments of Pediatric, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
HEBA
AMINOU
3
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
AUTHOR
AYMAN
BADR
4
Ministry of Health and Population, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
PREDICTIVE VALUE OF PHOSPHORYLATED INSULIN–LIKE GROWTH FACTOR BINDING PROTEIN-1(PIGFBP-1) (BEDSIDE TEST) IN PRETERM LABOR
This study evaluated the predictive value of phosphorylated insulin like growth factor binding protein-1 in cervical secretion as bedside test for prediction of preterm labor in symptomatic women. A total of 57 patients with singleton pregnancy at 24-34 weeks gestation, with symptoms suggestive of preterm labor were included in this study. A rapid cervical sample for PIGFBP-1 determination (Actim partus test, Medix Biochemical, and Kaunianen, Finland) was taken bymeans of a polyester-tipped swab during a speculum examination of the cervix, and extracted with specimen- extraction solution. We analyzed the prevalence of preterm labor in these patients within seven days upon admission. And calculate sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for PIGFBP-1. This study was done at Kuwait Oil Company hospital (KOC) and Taiba hospital (Kuwait) during the period between April 2011 and June 2012.The test was positive in 50.9% of patients and negative in 49.1%, among those tests was positive64% delivered less than a week, and among those tests was negative 35.7 delivered lessthan one week, with 74.3% sensitivity, 61% spesivity, 76.3% PPV and 73.6% NPV.
https://jesp.journals.ekb.eg/article_90413_1914e037458fb0982e4588edb9d1e380.pdf
2014-08-01
525
530
10.21608/jesp.2014.90413
phosphorylated insulin –like growth factor binding protein
bedside test
preterm labor
AMRO
ABO EL-EZZ
1
Department of Obstetrics and Gynecology Faculty of Medicine, AL-Azhar University, Nasr City, Egypt.
AUTHOR
ALSAEED
ASKAR
2
Department of Obstetrics and Gynecology Faculty of Medicine, AL-Azhar University, Nasr City, Egypt.
AUTHOR