EXPERIMENTAL EFFECT OF FEEDING ON RICINUS COMMUNIS AND BOUGAINVILLEA GLABRA ON THE DEVELOPMENT OF THE SAND FLY PHLEBOTOMUS PAPATASI (DIPTERA: PSYCHODIDAE) FROM EGYPT
RANIA
KALDAS
Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3 (NAMRU-3),
Cairo, Egypt.
author
AZZA
EL SHAFEY
Department of Botany, Faculty of Science, Ain Shams University,
Cairo, Egypt.
author
MAGDI
SHEHATA
Department of Entomology, Faculty of Science, Ain Shams University,
Cairo, Egypt.
author
ABDALLAH
SAMY
Department of Entomology, Faculty of Science, Ain Shams University,
Cairo, Egypt.+
author
JEFFREY
VILLINSKI
Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3 (NAMRU-3),
Cairo, Egypt.
author
text
article
2014
eng
Plants are promising sources of agents useful for the control of vectors of human diseases including leishmaniasis. The effect of Ricinus communis (Euphorbiaceae) and Bougainvillea glabra (Nyctaginaceae), on transmission of leishmaniasis was investigated using them as diets for Phlebotomus papatasi to monitor their effect on life-history traits. P. papatasi were allowed to feed separately on both plants then offered a blood-meal. Fed-females were observed daily for egg-laying and subsequent developmental stages. P. papatasi was able to feed on B. glabra (29.41% females and 46.30% males) and R. communis (5.80% females and 10.43% males). 34.28% of females died within 24-48 hours post-feeding on R. communis, whereas, it was 16.5% in females fed on B. glabra. Overall fecundity of surviving females was reduced compared to controls, reared on standard laboratory diet; however there was no effect on the sex ratio of progeny. FemaleP. papatasi in the control group had significantly longer life span compared to plant-fed group. Feeding on these plants not only decreased sand fly survival rates but incurred negative effects on fecundity. Findings indicate that planting high densities of R. communis and B. glabra in sand flies-endemic areas will reduce population sizes and reduce the risk of Leishmania major infections.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
1
12
https://jesp.journals.ekb.eg/article_90689_79eda485f623fda951a41a0b31613fa5.pdf
dx.doi.org/10.21608/jesp.2014.90689
ISOLATION OF STEM CELLS FROM THE MID GUT EPITHELIUM OF CULEX PIPIENS MOSQUITOES (DIPTERA: CULICIDAE)
N.
WASSIM
Molecular Biology Unit, Faculty of Science, Suez University, Suez, Egypt.
author
B.
SOLIMAN
Molecular Biology Unit, Faculty of Science, Suez University, Suez, Egypt.
author
M.
HUSSEIN
Molecular Biology Unit, Faculty of Science, Suez University, Suez, Egypt.
author
H.
METWALY
Department
of Hematology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt.
author
text
article
2014
eng
Stem cells are undifferentiated cells that remarkable ability to self-renew and produce differentiated cells that support normal development and tissue homeostasis. The authors isolated spherical stem cells from the midgut epithelium of the late third larval instars of Culex pipiens mosquitoes. In vitro, cultivated epithelial stem cells were able to proliferateand differentiate in fresh culture for 48 hr in absence of 20- hydroxyecdysone (20-E). The stem cells were the most common cells in the initial culture. One hundred of epithelial stem cells had been cultured. After 24 hr became 132 cells, 78% was undifferentiated stem cells and 8.3% &13.7% was enterocytes and goblet cells respectively. This ratio slightlydecreased after de 48 hr to be 74% undifferentiated stem cells, 4.5 % enterocytes and 5.6 % goblet cells. After 72 hr the undifferentiated stem cells were not able to proliferate .The most differentiated goblet and enterocytes survived few days then died.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
13
20
https://jesp.journals.ekb.eg/article_90691_cce505bf6ba71211f745f4b9678b169d.pdf
dx.doi.org/10.21608/jesp.2014.90691
PHENOLIC CONTENT OF SOME SELECTED LAMIACEOUS EGYPTIAN MEDICINAL PLANTS: ANTIOXIDANT POTENTIAL AND ECOLOGICAL FRIEND MOSQUITO-LARVICIDAL
NEVEIN
ABDEL-HADY
Departments of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar
University, Egypt.
author
ATEF
EL-HELA
Departments of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar
University, Egypt.
author
TOSSON
MORSY
Department of Parasitology, Faculty of Medicine, Ain-Shams University,
Cairo 11566, Egypt.
author
text
article
2014
eng
Phenol compounds are naturally occurring biologically active compounds existing in all plants had received major medical concern so development in research focused on their extraction, identification and quantification have occurred over the last 25 years; they constitute an important source of antioxidants and were used to help human body to reduce oxidativedamage. Mosquitos-borne diseases constitute one of the major health problems worldwide. Control strategies involving pinpointing natural ecological friend, cheap and safe mosquitocides, mainly larvicides to stop their life cycle. Quantitative estimation of total phenol, flavonoids, phenylethanoid and iridoid contents of sixteen selected Lamiaceous Egyptian plants for screening of their antioxidant and mosquito larvicidal effects was carried out. The results showed that the most suitable medicinal plants used as antioxidants were Lavendula dentata L., Thymus capitatus L. and Thymus boveiBenth., which contain adequate mixture of total phenol, flavonoid and phenylethanoid contents, with distinct larvicidal effect in a descending order was T. capitatus L., T. bovei Benth. and L. dentata L. by their adequate mixture of total phenol, flavonoid, iridoid and phenylethanoid glycoside content.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
21
24
https://jesp.journals.ekb.eg/article_90693_0f125465f47e26becf99151f6dabbe4c.pdf
dx.doi.org/10.21608/jesp.2014.90693
REDISCOVERY OF MERISTASPIS LATERALIS (KOLENATI) (ACARI: MESOSTIGMATA: SPINTURNICIDAE) PARASITIZING THE EGYPTIAN FRUIT BAT, ROUSETTUS AEGYPTIACUS (GEOFFROY) (MAMMALIA: CHIROPTERA), WITH A KEY TO MITES OF BATS IN EGYPT
MOHAMED
NEGM
Department of Plant Protection, Faculty of Agriculture, Assiut University, Assiut
71526, Egypt.
author
MAHMOUD
FAKEER
Department of Plant Protection, Faculty of Agriculture, Assiut University, Assiut
71526, Egypt.
author
text
article
2014
eng
Faunistic information about bat mites in Egypt is scarce. Collection records of parasitic mites, Meristaspis lateralis (Kolenati, 1856) (Mesostigmata: Spinturnicidae), are reported from the Egyptian fruit bat, Rousettus aegyptiacus (Geoffroy, 1810) (Mammalia: Chiroptera) in Assiut Governorate, Egypt. Seven species of bat mites are recognized from Egypt to date. A hostparasite checklist and an identification key to these species are presented.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
25
32
https://jesp.journals.ekb.eg/article_90694_2117ae8a1b615f03893a48c44e14e6f1.pdf
dx.doi.org/10.21608/jesp.2014.90694
ULTRASTRUCTURAL STUDY OF THE LATERAL LINE NEUROMASTS IN TADPOLES OF SAUDI BUFO DHUFARENSIS AND RANA RIDIBUNDA
GEHAN
FAHMY
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.
author
GAMAL
BEKHET
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.
author
text
article
2014
eng
Neuromast (hair cells) structure in Bufo dhufarensis and Rana ridibunda larvae was observed by Scanning Electron Microscopy (SEM). Neuromasts were found arranged in one well-defined line in the head, body, and tail regions forming the lateral line and also found haphazardly scattered in most of the body parts. Their number was significantly high in the head region, and then it gradually decreased along the posterior end of the body. The structure of neuromasts in these three regions was basically similar for each species. In Rana, neuromasts were found few in number, either spherical or oval in shape lacking hair-like structure except in the tail region where hair cells were found. While in Bufo, neuromasts were numerous. Long kinocilia and many stereocilia were found in the neuromasts. Kinocilia were either solitary or in clusters. In addition to the main functions of the neuromasts we discovered a new function which was not found in previous researches, neuromasts were also used to remove any attached object on the tadpole's skin, by directing thekinocilium to the object thing and rolling onto it then detaching it outwards.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
33
39
https://jesp.journals.ekb.eg/article_90697_d48ac8fca9558d4b413e6cb0a8174200.pdf
dx.doi.org/10.21608/jesp.2014.90697
SELECTED INFECTIOUS DISEASE DISASTERS FOR NURSING STAFF TRAINING AT EGYPTIAN EASTERN BORDER
MAMDOUH
EL-BAHNASAWY
Military Medical Academy, Egypt.
author
NARGIS
LABIB
Department of Public Health, Faculty of Medicine, Cairo
University, Egypt.
author
MAGDA
ABDEL-FATTAH
Department of Nursing
Administration, and Nursing, Cairo
University, Egypt.
author
ABEER
IBRAHIM
Military Medical Academy, Faculty of Medicine, Egypt.
author
TOSSON
MORSY
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Infectious disease disasters are events that involve a biological agent, disease and that result in mass casualties, such as a bioterrorism attack, an emerging outbreak of infectious disease; all disasters pose a risk of infection transmission. But, infectious disease disasters pose the great-risk to illness or death from an infectious disease. This study raised theawareness and improved knowledge by educational program for Military Nursing Staff on selected infectious disease disasters acquired at Egyptian Eastern Border. The selected arthropod- borne diseases were Anthrax, Tick borne relapsing, Louse borne replasing fever and liver fluke; Clonorchis sinensis. An interventional study was used, for 125 staff nursewho accepted to participate. The tools dealt with four questionnaires: (1) Some sociodemographic characteristics data (2) Educational needs assessment a structured questionnaire. (3) Knowledge test (pre/ post-test) and (4) Participants' reactions questionnaire. The results showed that educational intervention significantly improvements the nursing staff knowledge, which were achieved at the immediate post intervention phase, and retained via three months post-test phase. In the service training programs about infectious disease disasters at Egyptian Eastern Border must be established and continued on regular basis. This would improve their knowledge about the epidemiology of these infectious diseasedisasters.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
41
54
https://jesp.journals.ekb.eg/article_90700_b016a0749d7717a9893dad61b39bfbef.pdf
dx.doi.org/10.21608/jesp.2014.90700
HELMINTH PARASITIC INFECTIONS OF THE CENTRAL NERVOUS SYSTEM: A DIAGNOSTIC APPROACH
AHMAD
OTHMAN
Department of Medical Parasitology, Faculty of Medicine, Tanta University, Egypt.
author
FABRIZIO
BRUSCHI
Department of Translational Research and New Technologies in Medicine and Surgery,
University of Pisa, 56126 Pisa, Italy.
author
AHMED
GANNA
Department of Neurosurgery, Faculty of
Medicine, Tanta University, Egypt.
author
text
article
2014
eng
Helminth parasitic infections of the central nervous system (CNS) occur worldwide with high prevalence in tropical and subtropical countries. Clinical evaluation of patients is mandatory, and it is convenient to group the clinical manifestations into syndromes: for example space-occupying lesions, meningitis, and encephalitis. The history should focus on residence or travel to endemic areas, diet, activities, intercurrent medical conditions, and associated clinical clues. Direct parasitological diagnosis can be reached by cerebrospinal fluid and cerebral tissue examination either by microscopy, culture, or immunological techniques. Immunodiagnosis by detection of parasite antibodies or antigens in serum couldprovide indirect evidence of parasitic infections. In addition, various imaging and radiological techniques e.g., computed tomography (CT) scan and magnetic resonance imaging (MRI) complement the diagnostic work-up of CNS diseases. Finally, the helminthic CNS infections of global impact, such as schistosomiasis, neurotoxocariasis, Strongyloides infection,neurotrichinosis, neurocysticercosis, and echinococcosis will be briefly discussed as regards the principal clinical and diagnostic features.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
55
70
https://jesp.journals.ekb.eg/article_90704_51e0a7ae8ad24f9084e5d82006719829.pdf
dx.doi.org/10.21608/jesp.2014.90704
DISTINGUISHING THE TWO FORMS OF EGYPTIAN AEDES (OCHLEROTATUS) CASPIUS PALIAS SPECIES (DIPTERA: CULICIDAE) BY ULTRA STRUCTURE MICROGRAPHS OF EGGS
B.
SOLIMAN
Department of Zoology, Suez University, Suez, Egypt.
author
N.
WASSIM
Department of Zoology, Suez University, Suez, Egypt.
author
J.
LINLEY
Florida Medical
Entomology Laboratory, Institute of Food and Agricultural Sciences,
University of Florida, USA.
author
text
article
2014
eng
Ultrastructure of the two forms autogenous and anautogenous eggs of Aedes (Ochlerotatus ) caspius of Egypt are described using Scanning Electron Microscope (SEM). The eggs of the two forms are slightly boat shape with quite difference in width. Chorionic cells of the ventral surface are ultimately different in both forms in shape, width of reticulum , number and size of tubercles. The chorionic cells of the autogenous form's egg are elongate, narrow and almost curved with unusually wide, outer reticulum contain 2 - 13 large tubercles along with a few number in small size. However, the anautogenous form's egg , the chorionic cells of the ventral surface fairly distinct, very regular in outline with thin reticulum and usually hexagonal, each cell contain one or two large tubercles with many small scattered peripheral tubercles. Fine structure micrographic work of eggs of the Egyptian Ae. caspius provides new morphological evidence that both autogenous and unautogenous forms are certainly different and suggests that those forms are two distinct species.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
71
77
https://jesp.journals.ekb.eg/article_90705_8deefe0f3f49f0784ba5c9b5bc85ea8c.pdf
dx.doi.org/10.21608/jesp.2014.90705
ZOONOSES FROM PETS HORSES, DONKEYS AND MULES: WITH SPECIAL REFERENCES TO EGYPT
AYMAN
MORSY
Consultant of Tropical Medicine, The Ministry of Interior Hospitals, Cairo, Egypt.
author
MOSTAFA
SALEH
Consultant
of Parasitology, Military Medical Academy, Egypt.
author
TOSSON
MORSY
Department of Parasitology, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
A zoonosis is an animal disease that is transmissible to humans. Humans are usually an accidental host that acquires disease through close contact with an infected animal, who may or may not be symptomatic. Children are at highest risk for infection because they are more likely to have close contact with pets. Pets are responsible for transmission of an extensive array of bacterial, fungal, and parasitic zoonotic pathogens. The route of transmission can be through the saliva (e.g., bites or contaminated scratches), feces, respiratory secretions, direct contact, or by the animal acting as a vehicle and source of tick or flea exposure. Although pets have been implicated in transmission of zoonoses to their owners, riskof transmission from contact with pets is low and may be further reduced by simple precautions.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
79
96
https://jesp.journals.ekb.eg/article_90707_3a2a84eaa0b9f65d93d0740351d90733.pdf
dx.doi.org/10.21608/jesp.2014.90707
DIAGNOSTIC EVALUATION OF HEPATOPULMONARY SYNDROME IN EGYPTIAN CHILDREN WITH CHRONIC LIVER DISEASE
MOHIEE EL-DEEN
AWAD
Department of Pediatrics, Faculty of Medicine, Tanta University, Egypt.
author
HANAA
EL-ARABI
Department of Pediatric Hepatology, Faculty of Medicine, Menoufia
University, Egypt.
author
KHALED
EL-SHARNOUBY
Department of Cardiology, Faculty of Medicine, Tanta University, Egypt.
author
KHALED
ABO DEWAN
Department of Radiology,
Faculty of Medicine, Tanta University, Egypt.
author
text
article
2014
eng
There are few data on prevalence of hepatopulmonary syndrome (HPS) in children with chronic liver disease (CLD). This prospective study evaluated the prevalence and diagnostic procedures of HPS in Egyptian children with CLD. One hundred twenty (120) children with CLD were subjected to room-air pulse oximetry in supine and upright position, contrast enhanced echocardiography (CEE) and technetium-99m–labeled macroaggregated albumin (99mTc-MAA) perfusion lung scan. Arterial blood gas (ABG) analysis in upright position was performed for all children with identified intrapulmonaryvascular dilatation (IPVD). Clinical, laboratory, imaging and endoscopic data were recorded and analyzed. Results: Hypoxemia was found in 14 cases (11.7%) of the total cohort all of them had IPVD, whereas 6 cases (5%) of the patients had IPVD without hypoxemia. Therefore, HPS and subclinical HPS were diagnosed in 11.7% and 5% of CLD patients, respectively. Only 10 HPS patients had a pathological arterial oxygen saturation (SaO2) in the supine position (≤97%) but all showed a pathological SaO2 decrease (≥4%) after changing from supine to upright position.99mTc-MAA perfusion lung scan revealed IPVD in 16.7% whereas CEE detected IPVD in 10% only of enrolled patients. There were strong correlations between shunt index estimated by lung scintiscan and oxygenation parameters in HPS patients. The characteristics of HPS patients were similar to that of non-HPS patients except for clubbing, dyspnea, cyanosis, orthodoexia and bleeding varices that were more associated with HPS patients as well as well as the Child-Pugh grades, which tended toward higher scoresin HPS patients.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
97
112
https://jesp.journals.ekb.eg/article_90709_9829f24ffe45b65accb8f9d068c054d9.pdf
dx.doi.org/10.21608/jesp.2014.90709
ULTRASTRUCTURAL STUDY ON BIOMPHALARIA ALEXANDRINA HAEMOCYTES INFECTED WITH SCHISTOSOMA MANSONI IN EGYPT AND ITS CORRELATION WITH NITRIC OXIDE LEVEL
EMAN
HELAL
Department of Zoology, Faculty of Science, Al-Azhar University (Girls), Egypt.
author
SHADIA
EL- DAFRAWY
Department
of Medical Malacology, Theodor Bilharz Research Institute
P.O. Box 30, Imbaba, Giza, Egypt.
author
AMIRA
MOHAMED
Department
of Electron Microscopy, Theodor Bilharz Research Institute
P.O. Box 30, Imbaba, Giza, Egypt.
author
BASMA
ABOU-EL-NOUR
Department of Zoology, Faculty of Science, Al-Azhar University (Girls), Egypt.
author
SAMAH
IBRAHIM
Department
of Medical Malacology, Theodor Bilharz Research Institute
P.O. Box 30, Imbaba, Giza, Egypt.
author
text
article
2014
eng
Some snails of Biomphalaria alexandrina can resist the infection of Schistosoma mansoni so this study aimed to clearly this mechanism by using light and electron microscopy (EM) and determine the role of Nitric oxide in this mechanism. B. alexandrina snails used in this study were exposed individually to S. mansoni infection according to their response theywere classified into susceptible group (shed cercariae) and resistant group (failed to shed cercariae). Snails not exposed to infection were included in this study as control group. Nitric oxide (NO) level was assayed directly in the soluble fraction of B. alexandrina haemolymph supernatants collected from each group of B. alexandrina snails were subjected to NO assay by the Greiss reaction. The level of NO in haemolymph of infected snails was significantly increased (p<0.001) than both control and non infected snails groups, however, in non infected snails group had significantly (p<0.05) compared to control group. This study when correlated the changes recognized by EM with NO level the pro apoptotic effect of highlevel of NO on the haemocytes. Characterization and identification of cell shape of haemocytes in both haemolymph and tissue were examined by light and electron microscopy. Examination of B. alexandrina snail’s haemocytes revealed three types of different cells classified according to their shape and granular contents. These cells are granulocytes, amoebocytes and hyalineocytes. Electron microscope study also revealed the important role of granulocytes and amoebocytes as defense mechanism against snail infection. NO is considered an important anti parasite molecule; intra-molluscan stages of parasites switch off host NO defense response
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
113
124
https://jesp.journals.ekb.eg/article_90714_9aa0a225b877d2e396f4d860e50cba8d.pdf
dx.doi.org/10.21608/jesp.2014.90714
THE USING OF LUCILIA CUPRINA MAGGOTS IN THE TREATMENT OF DIABETIC FOOT WOUNDS
MOSTAFA
HASSAN
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr
City, Cairo, Egypt.
author
KOTB
HAMMAD
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr
City, Cairo, Egypt.
author
MOHAMAD
FOUDA
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr
City, Cairo, Egypt.
author
MOHAMMAD
KAMEL
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr
City, Cairo, Egypt.
author
text
article
2014
eng
In the present study, Lucilia cuprina maggots were used for the treatment of diabetic foot wounds. An artificial wound was made in diabetic foot of rabbit. The maggots were sterilized and put directly on the wound after dressing the wound without using any antibiotics. Several cycles of maggots were put on the wound. The present results showed that the treatment of the diabetic foot was observed after 13 days. After this period the wound was completely healed and become free of microbial contamination. The new tissues were observed to close the wound.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
125
129
https://jesp.journals.ekb.eg/article_90716_57ec581fe32ff5fb4ed39f36b621b9c0.pdf
dx.doi.org/10.21608/jesp.2014.90716
UNUSUAL LOCALIZATION AND PRESENTATION OF NONVISCERAL HYDATID CYSTS: REVIEW ARTICLE
REFAAT
KHALIFA
Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.
author
RAGAA
OTHMAN
Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.
author
text
article
2014
eng
Echinococcosis is a major parasitic zoonosis of public health importance worldwide. This is particularly true in sheep-raising countries including Egypt. Therefore, it is very important to identify the significant risk factors of the diseases by reviewing studies done in the region in the past decade to help policy makers design appropriate control strategies.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
131
140
https://jesp.journals.ekb.eg/article_90719_c330ea197bd32b2e38306c880a2528cb.pdf
dx.doi.org/10.21608/jesp.2014.90719
MODULATION OF TUBERCULOSIS-RELATED IMMUNE RESPONSES BYHELMINTHS
P.
MÉNDEZ-SAMPERIO
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Prol.
Carpio y Plan de Ayala, México, D.F. 11340 México.
author
text
article
2014
eng
Tuberculosis (TB) continues to be a major worldwide health problem, with 9.4 million newly emerging active tuberculosis cases and causes nearly 2 million deaths annually. Currently, experimental evidence for an strong association between helminths and diminished T helper (Th)1 immunity against Mycobacterium tuberculosis infection is based on studieswhich show that helminth-induced Th2, T regulatory (Treg) responses and alternatively activated macrophages contribute to enhanced susceptibility to TB. In this context, it has been shown that Th1 response is reduced in helminth coinfected TB patients. This article discusses what is presently known about the types of immune responses modulated by helminths to diminish the protective immune response to TB.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
141
144
https://jesp.journals.ekb.eg/article_90720_a2f06388ba3ec322931ca1f766733b38.pdf
dx.doi.org/10.21608/jesp.2014.90720
COMPARISON BETWEEN OMENTOPLASTY AND PARTIAL CYSTECTOMY AND DRAINAGE (PCD) TECHENQUES IN SURGICAL MANAGEMENT OF HYDATID CYSTS LIVER IN ENDEMIC AREA (YEMEN)
MARWAN
BORHAM
Department of Surgery, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
text
article
2014
eng
Hydatisosis caused by Echinococcus granulosus is more or less endemic in all sheep raising countries. This study evaluated omentoplasty versus partial cystectomy with drainage in surgical management of hydatid cysts of the liver in endemic area (Yemen). A total of 60 patients with hydatid cyst in the liver was divided into two groups; G1 (32 patients) treated with omentoplasty and G2 (28patients) treated with partial cystectomy with drainage (PCD). The results showed that the wound sepsis was seen in 6.25 %, of G1and in 14.2% of G2, biliary leakage occurred in 3.5% of G2, intra-abdominal abscess formation occurred in 3,5% of G2, atelectasis was in 3.1% of G1 and in 3.5% of G2. The total morbidity was12.5% in G1 and 32.1% in G2. There was a significant difference between the two groups as regard to the mean post-operative hospital stay, (6.5 days in G1 versus 15.6 days in G2).
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
145
150
https://jesp.journals.ekb.eg/article_90721_a85e778be85b395e479e2307f736fdc2.pdf
dx.doi.org/10.21608/jesp.2014.90721
FLUID OPTIMIZATION WITH HYDROXYETHYL STARCH 130/0.4 COMPARED WITH MODIFIED FLUID GELATIN GUIDED BY ESOPHAGEAL DOPPLER DURING MAJOR ABDOMINAL SURGERIES
GEHAN
EL-FANDY
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
SOHAILA
OMAR
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
AYMAN
EL-DESOUKY
Department of Anesthesiology, Faculty
of Medicine, Cairo University, Ministry of Higher Education, Egypt.
author
HANAN
KHAFAGY
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
MOHAMED
HUSSIEN
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
HEND
KAMEL
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
AHMED
REFAAT
Department of Anesthesiology, Theodor Bilharz Research Institute, Ministry of Scientific
Research, Imbaba P.O. 30, Giza, Egypt.
author
text
article
2014
eng
Perioperative fluid therapy has a direct bearing on patient outcome and accordingly should be tailored individually. Thus the efficacy of HES 130/0.4 was compared to modified fluid gelatin for volume expansion during major abdominal surgery guided by transesophageal Doppler (TED). Fifty adult patients ASA physical status I-II undergoing major abdominal surgery were anesthetized with standard technique. In addition to basal fluid requirement, patients were randomly allocated into two equal groups (25 patients each) to receive 200 cc of either 6% HES 130/0.4 (HES group) or 3% modified fluid gel (GEL group) as intraoperative colloid replacement guided by TED. Heart rate, mean arterial blood pressure, central venous pressure, and Doppler derived measurements were recorded at the following timings: T1; after induction, T2; after skin incision, T3; two hours after that and T4; at the end of surgery. Fluid administration and transfusion requirements were recorded. Laboratory tests for hemostasis, hepatic and renal functions were continued till the fifth postoperative day. Both groups were comparable regarding Doppler derived data and fluid balance. Platelet count showed a significant drop (p<0.05) in group GEL in all postoperative days compared with baseline and with the group HES. Prothrombin time and INR showed a significant increase while prothrombin concentration showed a significant drop, throughout 5 postoperative days in group HES while in the 3 postoperative days in group GEL (p<0.05) but comparable between groups. Both groups showed postoperative drop in creatinine level and postoperative rise of liver function tests. HES 130/0.4 and modified gelatin have comparable fluid optimization effect guided by TED in major abdominal surgeries. HES 130/0.4 has a more favorable effect on platelet counts than modified gelatin.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
151
160
https://jesp.journals.ekb.eg/article_90725_6b898f5e58adc30b6a9e55720e2268c1.pdf
dx.doi.org/10.21608/jesp.2014.90725
SOME STUDIES ON CAPILLARIA PHILIPINENSIS AND ITS MYSTERIOUS TRIP FROM PHILIPPINES TO EGYPT (REVIEW ARTICLE)
REFAAT
KHALIFA
Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.
author
RAGAA
OTHMAN
Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.
author
text
article
2014
eng
Capillaria philippinensis is a mysterious parasite and intestinal capillariasis is a mysterious disease. It is now more than half a century since the discovery of the first case in Philippines without answering many questions concerning the parasite's taxonomy, morphology, life cycle, diagnosis, pathology, clinical symptoms, mode of transmission as well as how it was transported to Egypt and how it started to spread and progressed in most Egyptian Governorates; particularly those of Middle Egypt. This article is a trial to overview all these aspects of the parasite.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
161
171
https://jesp.journals.ekb.eg/article_90728_358f59d6ae0f90221294cc9a20f700f6.pdf
dx.doi.org/10.21608/jesp.2014.90728
FAUNA OF MOSQUITO LARVAE (DIPTERA: CULICIDA) IN ASIR PROVENCE, KINGDOM OF SAUDI ARABIA
HAMDY
AL ASHRY
Environmental Balance Co., Alrawdah District, Jeddah, Kingdom of Saudi Arabia.
author
MOHAMED
KENAWY
Department of Entomology, Faculty of Science, Ain Shams University, Cairo 11566,
Egypt.
author
MOHAMMED
SHOBRAK
Biology Department, Science College, Taif University, Taif 21974, Kingdom of
Saudi Arabia.
author
text
article
2014
eng
An entomological survey was undertaken for one year to update the mosquito fauna of Asir Region, Kingdom of Saudi Arabia. A total of 31 species of 8 genera were reported of which genus Culex (55%) was the most common. Most of collected larvae (59%) belonged to genus Culex (+ Lutzia) followed by Culiseta (26%), Anopheles (13%) and Aedine spp.(2%). Cx. pipiens (39%) and Cs. longiareolata (26. %) were generally the most abundant of all collected larvae. Of the Anopheles spp., An. dthali was common (40%), of Culex spp., Cx. pipiens was predominating (66%) and of Aedine spp., St. aegypti was predominating (71%). Four species: An. fluviatilis, Cx. mattinglyi, Cx. arbieeni and Cx. mimeticus were new reports in Asir Region and Cx. wigglesworthi recorded for the first time from the kingdom. Larvae were more common in low- and highlands than in the moderately altitude areas. In general all species prefer stagnant water but with the exception of Aedine larvae (altogether), the other species prefer presence of algae, vegetation and shade and absence of turbidity (except Culex spp.). A total of 98 different forms of association were reported of which 9 forms were common. All genera breed year round with peaks of abundance during spring for Anopheles spp. and Culex spp. and during winter for Aedine spp. and Cs. longiareolata. A complete list of mosquito fauna of Asir Region comprising 45 spp. was presented based on the present and previous surveys. The study concluded that the occurrence and prevalence of mosquito species mainly the disease vectors in Asir carry the thread of maintaining and transmission of several mosquito-borne diseases.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
173
186
https://jesp.journals.ekb.eg/article_90729_1fe14f75686b8329786f8c9ab4e69467.pdf
dx.doi.org/10.21608/jesp.2014.90729
ASSESSMENT OF THE CO-INCIDENCE BETWEEN NON ALCOHOLIC FATTY LIVER DISEASE AND CAROTID ATHEROSCLEROSIS
SOHAIR
EL-SAYED
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11341, Egypt.
author
RUNIA
EL-FOLLY
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11341, Egypt.
author
AMR
AHMED
Department of Radiology,
Faculty of Medicine, Ain Shams University, Cairo 11341, Egypt.
author
text
article
2014
eng
Non-alcoholic fatty liver disease (NAFLD) is currently the most common cause of abnormal liver biochemistry and cryptogenic cirrhosis. Those with NAFLD have a higher prevalence of atherosclerosis, as shown by increased carotid artery intimal media thickness (CIMT). The aim of this study is to assess the co-incidence and prevalence between NAFLD and carotid atherosclerosis. In this study seventy-two subjects were categorized into 2 groups. GI: 52 patients diagnosed as NAFLD with diabetes mellitus type 2 or obesity or hyperlipedemia. GII: 20 diseased controls diagnosed as NAFLD without other predisposing factor. CIMT and plaque prevalence were estimated by carotid ultrasonography as a single trainedoperator who was blind to clinical characteristics of participants. The results showed that CIMT by carotid duplex ultrasonography was significantly higher in group A than group B but CIMT did not reveal any significant difference asregards to the etiology of NAFLD. CIMT was significantly higher in cases with bright liver than those with homogenous liver (by abdominal US) in group I and II. CIMT was significantly higher in those with moderate steatosis than those with mild steatosis (in GI & GII).
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
187
195
https://jesp.journals.ekb.eg/article_90732_265c9046967353dcdc2560abe1ac6909.pdf
dx.doi.org/10.21608/jesp.2014.90732
SECONDRAY STRUCTURE AND SEQUENCEOFITS2-rDNA OF THE EGYPTIAN MALARIA VECTORANOPHELES PHAROENSIS (THEOBALD)
NAHLA
WASSIM
Molecular Biology Unit, Faculty of Science, Suez University, Egypt.
author
text
article
2014
eng
Out of the twelve Anophelines present in Egypt, only five species known to be malaria vectors. Anopheles (An.) pharoensis proved to be the important vector all over Egypt, especially in the Delta. Anopheles sergenti proved to be the primary vector in the Oases of the Western Desert, An. multicolor in Faiyoum, An. stephensi in the Red Sea Coast, and An. superpictus in Sinai. Genomic DNA was isolated from single adult mosquito of An. pharoensis (Sahel Sudanese form), PCR was performed to amplify ITS2 region of rDNA using specific primers for 5.8S and 28S rDNA genes. The amplicons were purified, directly sequenced and aligned to the sequence of the same region of An. gambiae, using clustalw2. The length of ITS2-rDNA of An. pharoensis was 411bp. The GC content of the ITS2 reported 53% is consistent with spacer base composition in Anopheles species. The similarity between the two species was 52% and genetic distance was 0.46.Variable simple sequence repeats (SSRs) are found at low frequency. The secondary structure of rDNA-ITS2was predicted byMFOLD and was -192; 60 to-195.32 kilocalories/mole.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
197
204
https://jesp.journals.ekb.eg/article_90736_84765699778b43e51d480461d39b7131.pdf
dx.doi.org/10.21608/jesp.2014.90736
ROLE OF CEREBROSPINAL FLUID IL-8 AS A MARKER FOR DIFFERENTIATION BETWEEN ACUTE BACTERIAL AND ASEPTIC MENINGITIS
AMAL
ABDELMOEZ
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo11566, Egypt.
author
DOAA
ZAKY
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo11566, Egypt.
author
AMANY
MAHER
Medical Research
Center, Molecular Biology Unit, Ain Shams University, Cairo11566, Egypt.
author
text
article
2014
eng
No doubt, the distinguishing between bacterial and aseptic meningitis in the emergency department could help to limit unnecessary antibiotic use and hospital admissions. This study evaluated the role of cerebrospinal fluid IL-8 in differentiating acute bacterial meningitis (ABM) from aseptic meningitis (AM). A total of 80 hospitalized patients with clinical presentations of suspected acute meningitis were subjected to estimation of IL-8 CSF concentrations. The results showed that CSF IL-8 levels were higher in acute bacterial meningitis than in aseptic ones (p <0.05). The best cut-off value of CSF IL8 for early diagnosis of bacterial meningitis was 3.6ng/ml with a sensitivity of 82.5% and a specificity of85.0%.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
205
210
https://jesp.journals.ekb.eg/article_90739_01cb01b47d8f94b757483168ee92f1b7.pdf
dx.doi.org/10.21608/jesp.2014.90739
BOTULISM AS A FOOD POISONING: WHAT IS IT?
MAMDOUH
EL-BAHNASAWY
Military Medical Academy, Egypt.
author
NAGWA
ALY
Military Medical Academy, Egypt.
author
MAGDA
ABDEL-FATTAH
Department of Nursing Administration, Faculty of Nursing,
Cairo University, Egypt.
author
TOSSON
MORSY
Department of Parasitology, Faculty of Medicine, Ain
Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Botulism is a rare but potentially life-threatening neuroparalytic syndrome resulting from the action of a neurotoxin elaborated by the microorganism Clostridium botulinum. This disease has a lengthy history; the first investigation of botulism occurred in the 1820s with a case report on hundreds of patients with "sausage poisoning" in a southern Germantown. Several decades later in Belgium, the association was demonstrated between a neuromuscular paralysis and ham infected by a spore forming bacillus that was isolated from the ham. The organism was named Bacillus botulinus after the Latin word for sausage, botulus.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
211
220
https://jesp.journals.ekb.eg/article_90747_05aadb29e3b5e564ece56f805aa47d98.pdf
dx.doi.org/10.21608/jesp.2014.90747
ALLOGENIC HEPATOCYTE TRANSPLANTATION INIMMUNOMODULATED LEWIS RATS WITH ACUTE LIVER INSUFFICIENCY FOLLOWING HEPATECTOMY
MONA
MOHARIB
Departments of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Academy of
Scientific Research and Technology, Cairo, Egypt.
author
WALDEMAR,
OLSZEWSKI
Department of Surgical
Research and Transplantology Laboratories, Medical Research Center,
Polish Academy of Sciences, Warsaw, Poland.
author
NAGUI
MIKHAIL
Clinical and Experimental Surgery, Theodor Bilharz Research Institute, Academy of
Scientific Research and Technology, Cairo, Egypt.
author
MONA
NOSSIER
Department of Pathology, Theodor Bilharz Research Institute, Academy of
Scientific Research and Technology, Cairo, Egypt.
author
SHIMAA
ATTA
Department of Immunology, Theodor Bilharz Research Institute, Academy of
Scientific Research and Technology, Cairo, Egypt.
author
MOHAMED
SABER
Departments of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Academy of
Scientific Research and Technology, Cairo, Egypt.
author
text
article
2014
eng
Orthotropic Liver transplantation (OLT) is a conventional management for end-stage acute or chronic liver insufficiency, but the shortage of donor organs continues to be the restrictive factor throughout the world. Hepatocyte transplantation (HCTx) might be the promising treatment for several liver diseases and can be used as a "bridge" to OLT. Hepatocytes transplantation can protect and even save human lives, its’ applicability remains limited by the large deficiency of liver organs and hepatocytes (HC), and cellular loss after engraftment. Host elimination of grafted cells is called Early Graft Dysfunction. This study was developed for an efficient protocol of HCT. Several conditions have been met in order to achieve a high yield of harvested viable HC, overcome the detachedcell apoptosis, attenuation of innate immune reaction against transplanted cells and a receptive cell environment. HC were isolated from Lewis rats (n=8) weighing 250gm, bythe 2 step collagen a seper fusion technique, and bone marrow cells (BMCs) were obtained from the rats tibia and femur by centrifugation in a buffer solution. The mean viability of harvested HC and BMCs were 90% and 95% respectively.To minimize the rejection of HC, Lewis rat recipients (n=14) weighing 250gm, were irradiated with 6 Gy and received 0.1 mg of anti-aisle GM1 antiserum intravenously as immunosuppressive drug. The isolated HC were intra-splenically transplanted and 107 bone marrow cells were injected in a penile vein into the recipients on the third day. Simultaneously,70% hepatectomy and ligation of common bile duct were done. Thirty days later; the grafted spleen had areas with external appearance of a normal liver in ten out of 14surviving rats (71%). Hematoxlin and eosin (H & E) staining of sections from these fragments showed sinusoids and portal areas, an evidence of successful hepatocyte engraftmentand bile canaliculea formation. Large number of HC clusters of 15 to 20 cells and 2 to 4 distended small bile canaliculea were seen per50 HC. The intrasplenic route for transplanting freshly isolated HC in an immune-compromised animal model was found to give good results regarding cell engraftment and tissue formation.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
221
228
https://jesp.journals.ekb.eg/article_90750_86079ffc583fe8472b89218162ace48b.pdf
dx.doi.org/10.21608/jesp.2014.90750
THE USE OF MICROENCAPSULATED HEPATOCYTES TRANSPLANTATION REDUCES MORTALITY AND LIVER ALTERATIONS IN SCHISTOSOMA MANSONI INFECTED HAMSTERS
SOAD
SHERIF
Department of Biochemistry, Theodor Bilharz Research
Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.
author
MONA
MOHARIB
Department of Biochemistry, Theodor Bilharz Research
Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.
author
NAGLAA
EL-LAKKANY
Department of Pharmacology, Theodor Bilharz Research
Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.
author
OLFAT
HAMMAM
Department of Pathology, Theodor Bilharz Research
Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.
author
FATMA
SALMAN
Department of Biochemistry, Theodor Bilharz Research
Institute, Imbaba, P.O. Box 30, Giza 12411, Egypt.
author
MOHAMED
EL-NAGGAR
Department of Biochemistry,
Faculty of Science, Mansoura University, Egypt.
author
text
article
2014
eng
Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotropic liver transplantation. The goal of this work was to study the adequacy of intrasplenic hepatocyte transplantation (HCTx) in fresh and microencapsulated forms, in a hamster model of liver fibrosis by Schistosoma mansoni infected hamsters were divided into 6 groups; untreated for 11 weeks (GI) and for 15 weeks (GII), treated with praziquantel (PZQ) 7 weeks PI, and killed 4 weeks (GIII) and 8 weeks (GIV) post-treatment. Treated with PZQ 7 weeks PI, and then treated orally with immunosuppressive drug "cyclosporine (4 weeks post PZQ treatment), 24 hr. before interasplenic injection with fresh hepatocytes (V). Treated with PZQ 7 weeks PI, and then injected interasplenically (4 weeks post-treatment) with microencapsulated hepatocytes (GVI). GI & GIII were killed 11 weeks PI for assessment the anti-schistosomal efficacy of PZQ. The other four groups were killed 15 weeks PI for investigation of liver and spleen histology, serum liver enzymes and hepatic oxidative markers before and after HCTx. Freshly isolated hepatocytes with a mean viability 92.971.2% were used for microencapsulation and transplantation. Histological study showed the presence of transplanted hepatocytes in spleen of recipient. PZQ accelerated healing of hepatic granulomatous lesions as evidenced parasitologically by the increase in the percentage of dead eggs and histologically showing more granuloma circumscription with more ova degeneration and less inflammatory cells. The 25-day survival rates in GII, GIV, GV& GVI were 5/15 (33.3%), 8/15 (53.3%), 10/15 (66.7%) and 9/15 (60%) respectively. In addition, there were significantly better outcomes in serum biochemical indexes such as ALT, AST, -GT, ALP, and hepatic SOD and MDA in the fresh and microencapsulatedgroups than in PZQ-treated group, without great differences between the microencapsulated and the fresh transplanted groups. Liver pathological staining supported these findings.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
229
242
https://jesp.journals.ekb.eg/article_90752_124163faf64100828edd291943d137c5.pdf
dx.doi.org/10.21608/jesp.2014.90752
REPELLENT EFFECT OF LAGENARIA SICERARIA EXTRACTS AGAINST CULEX PIPIENS
MOSTAFA
HASSAN
Department of Zoology, Faculty of Science , Al-Azhar University, Nasr City, Cairo,
Egypt.
author
MOHAMAD
FOUDA
Department of Zoology, Faculty of Science , Al-Azhar University, Nasr City, Cairo,
Egypt.
author
KOTB
HAMMAD
Department of Zoology, Faculty of Science , Al-Azhar University, Nasr City, Cairo,
Egypt.
author
MOHAMAD
TANANI
Department of Zoology, Faculty of Science , Al-Azhar University, Nasr City, Cairo,
Egypt.
author
AHMED
SHEHATA
Department of Zoology, Faculty of Science , Al-Azhar University, Nasr City, Cairo,
Egypt.
author
text
article
2014
eng
Ethanolic, acetone and petroleum ether extracts from leaves and stems of Lagenaria siceraria (Cucurbitaceae) were screened for their repellency effect against Culex pipiens L. mosquito. The repellent action of the present plant extracts were varied depending on the plant parts and the dose of extract. The petroleum ether extract of leaves showed thesame repellency percent (100%) of commercial formulation, N. N. diethyl toulamide (DEET) at the higher dose (3.33 mg/cm2), while petroleum ether extract from stems exhibiting the repellent action (89.6%) at the same dose, respectively. Ethanolic extracts of leaves and stems exhibited the lowest repellent activity as it recorded (81.3% and69.1%) at (6.67 mg/cm2), respectively. Results of this study may contribute to design an alternative way to control mosquitoes currently based on applications of synthetic insecticides. These extracts could be developed commercially as an effective personal protection measure against mosquito bites and thus to control diseases caused by mosquito–borne pathogens.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
243
248
https://jesp.journals.ekb.eg/article_90754_c3362b6a6a392fc5a8ce62ad37f98c9b.pdf
dx.doi.org/10.21608/jesp.2014.90754
THE IMPACT OF FISH PARASITES ON HUMAN HEALTH (REVIEW ARTICLE)
OMAR
AMER
Department of Zoology, Faculty of Science, Al-Azhar University
(Assiut Branch), Egypt.
author
text
article
2014
eng
One of the most important problems faced by world at the present time is food deficiency. Today the third world is facing protein deficiency as one of the major global challenges. I Egypt, the continuous population explosion requires more food production to meet the consequent increasing demands. However, there are many zoonotic fish parasites not onlu in Egypt but worldwide.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
249
274
https://jesp.journals.ekb.eg/article_90756_455c968b849382d8d715f4a4b1df2d90.pdf
dx.doi.org/10.21608/jesp.2014.90756
EVALUATION OF SALIVARY AND SERUM ANTI-HELICOBACTER PYLORI IN EGYPTIAN PATIENTS WITH H. PYLORI RELATED GASTRIC DISORDERS
EFFAT
EL-FAKHFAKH
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
IMAN
MONTASSER
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
REHAM
KHALIFA
Department of Microbiology and Immunology,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Helicobacter pylori is a common and important transmissible bacterial human pathogen. Although several diagnostic tests are available for the detection of H. pylori infection, all of them have both advantages and disadvantages, and none can be considered as a single gold standard. Serological methods analyzing (serum and saliva) by using enzyme immunoassays,which are simple, reproducible and inexpensive, can detect either antigen or antibody. This study evaluated the frequency of anti- H. pylori serum and salivary antibodies positivity among Egyptian patients with gastric disorders and the validity of salivary, serum serological tests for diagnosis of H. pylori, comparing this with gold standard tests performed on endoscopy biopsy. This prospective, case-controlled study included 45 Egyptian patients who attended Ain Shams University Hospitals Cairo, Egypt between January 2013 and June 2013. There were 29 males &16 females their mean age was 51.78±7 (range 18-60). Among the ulcerogenic drugs, Aspirin was the most common drug (46.7%).The evidence revealed the sensitivity of Rapid Urease Test (RUT) was 100%, specificity was 71.4%, Positive Predictive value (PPV) was 88.6% and Negative Predictive value (NPV) was 100%. The sensitivity of serum IgG was 68.97% and specificity was 42.86%; while the sensitivity of serum IgA was 89.6 % and the specificity was 50 %. Correlating the salivary IgG results with H. pylori status diagnosed by culture, salivary IgG succeeded to diagnose 19 cases from the 31 positive H. pylori patients with a sensitivity of 63.33% & specificity of 92.86% whereas the results of salivary IgA showed a sensitivity of 80% and specificity of 92.86% .
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
1
no.
2014
275
283
https://jesp.journals.ekb.eg/article_90759_5826cd8667e1f3b4c5e8b91fc76c416a.pdf
dx.doi.org/10.21608/jesp.2014.90759