ELECTRON MICROSCOPIC STUDY OF THE DEVELOPMENTAL STAGES OF EIMERIA INTESTINALIS CHEISSIN, 1948 IN DOMESTIC RABBIT (ORYCTOLAGUS CUNICULUS) WITH REFERENCE TO ENDODYOGENY
HODA
ELFAYOUMI
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef,
Egypt.
author
HEBA
ABDEL-HALEEM
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef,
Egypt.
author
text
article
2014
eng
The endogenous stages in Eimeria intestinalis were studied in experimentally infected coccidia- free rabbits by transmission electron microscopy. Four asexual generations were observed. Two types of merozoites were reported. Binucleated merozoites possess all the characteristics of apical complex indicating asexual reproduction by endodyogeny. While the second type, the mononuclear merozoites have one central nucleus. Gamonts were developed mainly from the third generation merozoites where the mature gamonts were recorded together with the fourth generation schizonts. Macro- and microgametogenesis were clearly observed. Development of microgametes and its fine structural characteristics were detected. Mature macrogametes with central large nucleus and the appearance of two types of wall forming body (I, II) were observed. Many reserve food materials including amylopectin granules and lipids dropletswere very characteristic. Control experimentally infected rabbits shed unsporulated oocysts on the day eight p.i.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
531
538
https://jesp.journals.ekb.eg/article_90135_b126dc0c9e80638d60821f6f63d62a5f.pdf
dx.doi.org/10.21608/jesp.2014.90135
DIFFERENT TECHNIQUES FOR MANAGEMENT OF COMMON BILE DUCT STONES: A SINGLE CENTRE EXPERIENCE
MOHAMED
EL WAKIL
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
NADIA
ABDELKADER
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
HOSSAM EL-DEEN
SALEM
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
AHMED
ABO HALIMA
Department of Internal Medicine,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Local experience on the combined technique of endoscopic sphincterotomy followed by endoscopic balloon dilation is scarce. This study clarified whether this combined technique will offer any advantages, with respect to therapeutic outcome and complications rate, as compared with endoscopic sphincterotomy (ES) and endoscopic balloon dilatation (EBD) alone for the extraction of large and/or multiple common bile duct stones. For a total of 76 patients, extraction oflarge and/or multiple common bile duct (CBD) stones during endoscopic retrograde cholangiopancreatography was performed. According to the used technique, they were categorized into 3 groups; Endoscopic sphincterotomy, endoscopic balloon dilatation or combined technique. The success rate of complete stone removal and the incidence of procedure-related complications were compared among the three groups. Success rate after one session was recorded to be comparable among the three groups. Relative Risk Ratio assessment of success rate after single session among the three groups showed no statistically significant difference. Regarding bleeding, only 3 (10%) cases were recorded in the ES group with no cases in the other 2 groups. No significant difference was noted among the three groups regarding other complication. The combined technique of ES followed by EBD is an effective and safe technique enables extraction of multiple and/or relatively large stones. It could be a reasonable alternative option when standard techniques are inadequate to remove bile duct stones.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
539
546
https://jesp.journals.ekb.eg/article_90139_7ec72bd409868e83b441fa9e097251ea.pdf
dx.doi.org/10.21608/jesp.2014.90139
ENCYSTED METACERCARIAE OF FAMILY HETEROPHYIDAE IN INFECTED FISH IN DAKAHLIA GOVERNORATE, AN ENDEMIC FOCUS IN EGYPT
MAI
HEGAZI
Departments of Parasitology and Community Medicine & Public Health,
Faculty of Medicine for Girls Al-Azhar University, Cairo, Egypt.
author
AISHA
HASSAN
Departments of Parasitology and Community Medicine & Public Health,
Faculty of Medicine for Girls Al-Azhar University, Cairo, Egypt.
author
THANAA
AL-NASHAR
Departments of Parasitology and Community Medicine & Public Health,
Faculty of Medicine for Girls Al-Azhar University, Cairo, Egypt.
author
OMAIMA
ABO-ELKHEIR
Departments of Parasitology and Community Medicine & Public Health,
Faculty of Medicine for Girls Al-Azhar University, Cairo, Egypt.
author
FATMA
EL-LESSI
Departments of Parasitology and Community Medicine & Public Health,
Faculty of Medicine for Girls Al-Azhar University, Cairo, Egypt.
author
text
article
2014
eng
The study re-evaluated the status of encysted metacercariae (EMC) of Family Heterophyidae in fresh and brackish water fish in an endemic focus in Egypt, as well as to study their morphological pattern. Seasonal variation of EMC was matched with their prevalence and intensity in infected fish. The study covered a period of one year from first of April 2011 to end of March 2012. The total percent of infection in the examined fish was 71.9% and 42.6% for brackish and fresh water respectively. The highest prevalence of the recovered EMC in both sources of water was for Heterophyes heterophyes (57.9%), followed by Pygidiopsis genata (39.7%), other Heterophyes spp. (21.09%), Haplorchis pumilio (19.5%), Phagicola spp (19.5%) and Stictodora tridactyla (6.2%). The highest mean intensity of the recovered EMC in both sources of water was for Pygidiopsis genata (10.27±2.83), followed H. heterophyes (9.45±7.13), Haplorchis pumilio (1.76±2.03),Phagicola spp. (1.59±1.71), other Heterophyes spp. (0.82±0.99) and Stictodora tridactyla (0.48±0.92). By using ANOVA test, there was significant difference in sizes of EMC of Pyigidiopsis genata (P=0.04) and Phagicola spp. EMC (P=0.03) in comparison to corresponding ones in all fish species. By using Student T-test, there was significant higher size of Haplorchis pumilio EMC in Tilapia species in comparison to the corresponding one in Mugil species. Monthly variation showed that summer was the season with the highest prevalence and highest mean intensity of EMC in both brackish and fresh water fish. Also, undifferentiated EMC most properly of genus Centrocestus was demonstrated.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
547
558
https://jesp.journals.ekb.eg/article_90140_6570188c35fb561a46da8abd4e39f667.pdf
dx.doi.org/10.21608/jesp.2014.90140
PRESENT STATUS OF PROTOZOAN PATHOGENS CAUSING WATER-BORNE DISEASE IN NORTHERN PART OF EL-MINIA GOVERNORATE, EGYPT
REFAAT
KHALIFA
Departments of Parasitology, Faculty of Medicine, Assuit University, Egypt.
author
AZZA
AHMAD
Departments of Parasitology, Faculty of Medicine, El- Minia University, Egypt.
author
EKHLAS
ABDEL-HAFEEZ
Departments of Parasitology, Faculty of Medicine, El- Minia University, Egypt.
author
FADIA
MOSLLEM
Department of Public Health and Preventive Medicine, El-Minia University, Egypt.
author
text
article
2014
eng
Little is known about the role of different water supplies in the diversity and public health significance of pathogenic protozoan parasites. Most of these organisms have been ubiquitous in waters worldwide. The numbers of waterborne infections indicate a significant risk for their transmission even by drinking water. Hence, a total of 336 water sampleswere collected during 2009-2013 from different water sources from different areas of northern part of El-Minia Governorate, Egypt and were investigated for pathogenic protozoa. They were examined by direct microscopy followed by Modified Ziehl-Neelsen and Giemsa stains. 140 samples (41.7 %) were positive (statistically significant; P value P<0.0003). Prevalence rates were in Summer (66.7%), Spring (51.1%), Fall (26.2 %) and Winter (22.6%). These data were statistically significant (P<0.0001). The commonest protozoa detected as a single infection was Cryptosporidium sp. (53.17%) found in all water supplies, followed by Blastocystis hominis (15.87%), Cyclospora caytenensis (11.9%), Entamoeba histolytica/ dispar (8.73%) Giardia lamblia (6.35%) and Naegleria sp., (3.97%). Moreover, there were 14 samples with mixed parsitic infection: they were Cryptosporidium sp. and B. hominis in six samples, Cryptosporidium sp. and C. caytenensisin five samples and Cryptosporidium sp. and E. histolytica/dispar in three samples. The most common contaminated water source was ponds where 32 samples (66.7%) were positive followed by canal water 30 samples (62.5%). The results were discussed and the recommendations were offered.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
559
566
https://jesp.journals.ekb.eg/article_90143_567f67f83f12b544aa6673a7c27104db.pdf
dx.doi.org/10.21608/jesp.2014.90143
FARMERS HEALTH AND SAFETY AT SHARKIA GOVERNORATE AND THE INFLUENCING ENVIRONMENTAL FACTORS
WAGIDA
KAMEL
Department of Community Health, Faculty of Nursing, Zagazig University, Egypt.
author
EKRAM
AHMED
Specialist
Nursing, General Hospital Faqous, Ministry of Health and Population, Egypt.
author
TOSSON
MORSY
Department
of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Agriculture is one of the most hazardous of all economic sectors and many agricultural workers suffer occupational accidents and ill health each year.This study explored the most significant factors that impact on the health of farmers and the interrelationship between these factors. Cross-sectional descriptive study was conducted on 521 of farmers at Dawar-Gihenna village, Faqous District, Sharkia Governorate. Tools were used for data collection are an interview questionnaire consists of six parts to collect data socio-demographic characteristics of farmers, Economical status and home environment, Medical history, Knowledge regarding health and safety, Attitude regarding health and safety and Practice regarding health and safety. The fieldwork was executed in three months. It started in March 2013 to till the end of June2013. The results revealed that (58.2%) of participants had unsatisfactory knowledge, while (61.4%) had Positive attitude towards agricultural hazards and (51.1%) of participants had adequate practices towards agricultural hazards.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
567
590
https://jesp.journals.ekb.eg/article_90145_8057a0b898e3e8661a5b51faaf8b0c34.pdf
dx.doi.org/10.21608/jesp.2014.90145
INCIDENCE OF LEISHMANIASIS IN AL BAHA PROVINCE, SAUDI ARABIA: PAST AND PRESENT SITUATION (OBSERVATIONAL AND DESCRIPTIVE STUDY)
KHAIRY
MOHAMMAD
Department of Parasitology, Faculty of Medicine, Al-Baha University, Saudi Arabia,
(Al-Azhar University, Egypt)
author
NABEEL
ALHUSSAINY
Department of Medical Microbiology and
Parasitology, Faculty of Medicine, King Abdulaziz University, Saudi Arabia.
author
text
article
2014
eng
Cutaneous leishmaniasis (CL) is a worldwide skin protozoan infectious disease. This study evaluated the past and present situation of CL in Al-Baha Province. The incidence of CL patients over a total of (250,000) mid-year population was recorded from January to December in both years of 2012 and 2013. The statistical analyses were carried out with SPSS version 12.0 for windows. The highest number of patients was in 2012 with 11cases in this year. 37.5% of patients agedyounger than 15-year and 62.5% were older than 15 year. The highest incidence of disease was observed in Almandak Governorate in 2012 (8 over 250,000 mid-year population), and the lowest incidence rate was in Al-Tawlah Governorate in the same year. The disease was observed with the incidence rate over 250,000 mid-year population of 3,3,2,1,1and 1 for January, May , December, March, June and September 2012 respectively and 3,1, and 1 for June, January and April 2013 respectively. The highest incidence of disease was observed among males, Saudi patient who are resident in Al-Baha in both years of 2012 and 2013.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
591
596
https://jesp.journals.ekb.eg/article_90149_1d1b4328b839a5a02d4a425641fb7241.pdf
dx.doi.org/10.21608/jesp.2014.90149
CUTANEOUS LEISHMANIASIS IN BASRAH VILLAGES, SOUTH IRAQ
HIND
JARALLAH
Marine Science Center, University of Basrah, Basrah, Iraq.
author
text
article
2014
eng
CL in Iraq is caused by both Leishmania major and Leishmania tropica. Marshes Villages of Basrah province south Iraq are regarded as a rural area. A total of 35 cutaneous leishmaniasis cases 12 (34.28%) females and 23(65.71%) males were clinically registered from April 2013 to March 2014 in Health Care Center of Basrah Marshes Villages, South of Iraq are reported and investigated. All patients were parasitologically confirmed by stained smears and culture, the main clinical features were ulcerations. Number of cutaneous lesions per patients ranged from 1 to 5 with different lesions’ size from 3×3 mm to 25×25 mm in diameter. Amastigotes form were detected in all cases 35 (100%), while promastigotes from were found in culture sample of 29 (82.8%) cases. The results were analyzed and evaluated. Cutaneous leishmaniasis in Basrah marshes villages may differ from other regions because of the difference of the Leishmania strains, people life style, behaviors and difference in vectors and reservoir hosts.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
597
603
https://jesp.journals.ekb.eg/article_90150_dbe699657068c0db66f11e6ae3e6c235.pdf
dx.doi.org/10.21608/jesp.2014.90150
ASSESSMENT OF NURSES' PERFORMANCE RELATED TO CONTROL OF SOME PARASITES ACQUIRED FROM FRESH VEGETABLES AS A PATIENT SAFETY MEASURE IN A MILITARY HOSPITAL
AHMED
SALEH
Military Institute of Health and Epidemiology, The Military Medical Academy, Egypt.
author
HISHAM
ALI
The Military Medical Academy, Egypt.
author
SALWA
AHMED
Military Institute of Health and Epidemiology and Medical Surgical Nursing,
The Military Medical Academy, Egypt.
author
NAGLAA
MOHAMED
Military Institute of Health and Epidemiology and Medical Surgical Nursing,
The Military Medical Academy, Egypt.
author
TOSSON
MORSY
Department of Parasitology,
Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Foodborne parasitic infection in the hospital constitutes a major health problem particularly for patients who are more vulnerable than healthy subjects to parasitic risks. Parasitic infection represents an area of concern for advanced practice nurse. The work assessed the military nursing staff performance regarding the nosocomial food-borne parasitic infection control. Subjects and Methods: Research design; A descriptive research design was used to identify knowledge, attitudes and practice of nurses related to nosocomial food-borne parasitic infection control measures. Setting: The study was conducted at a general military hospital. Subjects: 50 nurses, the whole available number who covered the inclusion criteria. Data collection tools: It included; (1): Structured interview sheet was constructed after reviewing the relevant literature to elicit information, it included two parts a) subjects' sociodemographic characteristics b) nurses’ knowledge regarding nosocomial parasites infections. (2): Rating scale: to assess attitude of nursing staff towards nosocomial parasites infections and its prevention. (3): Observational Checklist: to assess the nurses’ practice of self-protection and control of nosocomial parasitic infections control measures. Results: A statistically insignificant difference between knowledge levels about nosocomial parasites infection among military nurses. More than half of participant nurses had positive attitude towards nosocomial parasite infection control and prevention without significant difference between all topics (P>0.056). That most participant nurses had unsatisfactory practice to infection control measures with significant lower score for procurement, storage and preparation of raw material followed by personal hygiene (P<0.05). Recommendations: Nurses in all roles and settings can demonstrate leadership in infection prevention and control by using their knowledge, skill and judgment to initiate appropriate and immediate infection control procedures.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
605
618
https://jesp.journals.ekb.eg/article_90151_66c873e29bf0b2e63ba78d7775e6c6fe.pdf
dx.doi.org/10.21608/jesp.2014.90151
HYDATID DISEASE OF THE LIVER: LAPAROSCOPIC APPROACH, INITIAL RESULTS IN EGYPT
AMR
ABDELAAL
Department Of General Surgery, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
author
HANY
DABBOUS
Department Of Tropical Medicine, Faculty of
Medicine, Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
This prospective study assessed the laparoscopic approach for treatment of HHC in Ain Shams University Hospitals from January 2010 to April 2012. Laparoscopic partial cystectomy was performed in all patients; no conversion to open technique or anaphylaxis was recorded. The mean operative time was 60 minutes with no perioperative mortality, while postoperative morbidity was recorded in 4 patients (36%). The mean length of hospital stay was 4.5 days. Radiological and serological tests showed no recurrences at a median follow up period of 18 months. Laparoscopic management of HHC is feasible gaining all the benefits of laparoscopy with no added morbidities or increased risk of recurrence. Careful patient selection is mandatory to achieve successful results.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
619
625
https://jesp.journals.ekb.eg/article_90152_488ea21ac4a70c45558c006f0f4bd191.pdf
dx.doi.org/10.21608/jesp.2014.90152
BIOLOGICAL AND HEMATOLOGICAL RESPONSES OF BIOMPHALARIA ALEXANDRINA TO MYCOBIOSYNTHSIS SILVER NANOPARTICLES
HODA
ABDEL-HAMID
Department OF Environmental Researches and Medical Malacology, Theodor
Bilharz Research Institute, Egypt.
author
AMAL
MEKAWEY
Regional Center for Mycology and
Biotechnology, Al-Azhar University, Cairo, Egypt.
author
text
article
2014
eng
Silver nanoparticles (AgPNs) extracts were prepared from seven Seven fungal isolates were evaluated through measuring their toxicity against Biomphalaria alexandrina snails. The effects of the two promising Paecilomyces variotii and Aspergillus niger AgNPs sublethal concentrations (LC10 & LC25) on the levels of steroid sex hormones, liver enzymes, total protein, lipids, albumin, glucose, total and differential count of hemocytes and morphology of hemocytes, oocytes and sperms were studied in this work. The short period of snails’ exposure (24h) to the two fungal AgNPs resulted in significant decrease in the levels of progesterone in B. alexandrina. The level of testosterone hormone showed significant increase in snails exposed to P. variotii AgNPs while no significant change was recorded at the exposure to A. niger AgNPs. Also, estradiol hormone concentration increased significantly in this investigation with the increase of theconcentration of the two tested compounds. In addition, significant elevation in ALT, AST and Alkaline phosphatase was recorded. The total number of the hematocytes increased significantly by17.4-47.8%. Snails’ granulocytes were reduced by 19.1- 43.8%, while hyalinocytes increased by 63.6-354.5%. The exposure of B. alexandrina to LC25 of both P. variotii and A. niger AgNPs showed apoptotic hemolymph cells, fragmented, vacuolated and degenerated cytoplasm, shrunken nucleus and phagocytosis in the light microscopy photographs of the hemocytes. Besides, the photographs showed also, abnormal nuclear division, degeneration and large fat vacuoles in the cytoplasm and swallowed atretic oocytes. Also, the photographs showed dead sperm head separated from its tail, other sperms showed abnormal swallowed head with severely nodded tail, dead sperms with wrinkled tails, hyperplasia and necrotic sperm heads led to overlapping oftails. In conclusion, applying the biosynthesized compounds which led to destruction of blood cells (the immune system), ova and sperms (the reproductive system) of snails is an important effective step to control schistosomiasis.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
627
637
https://jesp.journals.ekb.eg/article_90153_26af19577d043962322adedd33d57253.pdf
dx.doi.org/10.21608/jesp.2014.90153
ZOONOTIC MYIASIS IN EGYPT: WITH REFERENCE TO NOSOCOMIAL OR HOSPITAL-ACQUIRED MYIASIS
TOSSON
MORSY
Department of Parasitology, Faculty of Medicine, Ain Shams University,
Cairo 11566, Egypt.
author
text
article
2014
eng
The most important insects from medical point of view are the blood suckers or the insectborne diseases. Still others, almost non-blood suckers, but may attack man and animal to deposit their eggs or larvae of myiasis producing flies in skin, nose, eye, lung, ear, anus, vagina, and oral cavity as well as accidental gastrointestinal ones causing pathogenic condition known as myiasis causing different pathogenic conditions. Nosocomial myiasis must be noted carefully,especially in case of hospitalized patients. Myiasis is a real welfare problem and many myiasis producers are zoonotic parasites. The nosocomial myiasis illustrates an unusual problem that may confront those responsible for infection control programs. However, still little is known about such an important subject in Egypt. This review would assist in designing appropriate prevention protocols and devising suitable control strategies to overcome zoonotic and nosocomialmyiasis and alleviate the economic losses.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
639
652
https://jesp.journals.ekb.eg/article_90154_4c70a1577ed7c184df73bf5ee8746c9e.pdf
dx.doi.org/10.21608/jesp.2014.90154
A COMPARATIVE STUDY OF HAEMOCYTES FROM RESISTANT AND SUSCEPTIBLE LYMNAEA NATALENSIS SNAILS EXPOSED TO FASCIOLA GIGANTICA MIRACIDIA
KAMELIA
EL-SAYED
Department of Medical Malacology, Theodor Bilharz Research Institute, Egypt.
author
ABDEL-HAKIM
SAAD EL-DIN
Department of Zoology, Faculty of Science, Cairo University, Egypt.
author
RASHA
GAD EL-KARIM
Department of Medical Malacology, Theodor Bilharz Research Institute, Egypt.
author
text
article
2014
eng
Effect of infection with Fasciola gigantica on total and differential haemocytes count of resistant and susceptible Lymnaea natalensis snails were studied. Exposure of L. natalensis resistant and susceptible strains to F. gigantica on miracidia caused gradual increase in the number of circulating haemocytes at the same time of exposure. In susceptible strain, the increase in the number of circulating haemocytes became significant at the second week post exposure being 2560 cell/ml (p<0.05) in comparison with control group being 1760 cell/ml. While in resistant strains, the increase became significant at second, third and sixth weeks post exposure being 2660 cell/ml, 2500 cell/ml and 2360 cell/ml respectively,then a gradual decrease occurred. Examination of haemocytes obtained from L. natalensis snails revealed that haemolymph contained three morphological types of haemocytes, designated as round small, round large (hyalinoctyes) and granulocytes spreading. Their average percentage was 12.3±5.5%, 81.0±4.6% and 6.7±2.1% of total cells respectively. Data indicated that by the second day post exposure infected snails had significantly higher percentage of granulocytes than controls.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
653
660
https://jesp.journals.ekb.eg/article_90155_dc2e2b72cd00636b836c9616f8ae304e.pdf
dx.doi.org/10.21608/jesp.2014.90155
INTRAMEDULLARY SPINAL NEUROCYSTICERCOSIS TREATED SUCCESSFULLY WITH MEDICAL THERAPY
SHAMEEM
AHMED
Department of Neurosurgery, Guwahati Medical College and Hospital, Guwahati
(India), and Department of Neonatal Medicine, South mead Hospital, Bristol
(United Kingdom).
author
SIBA
PAUL
Department of Neurosurgery, Guwahati Medical College and Hospital, Guwahati
(India), and Department of Neonatal Medicine, South mead Hospital, Bristol
(United Kingdom).
author
text
article
2014
eng
Neurocysticercosis caused by Taenia solium and is a common parasitic disease of the cental nervous system. It usually presents with seizures, headaches, progressively worsening focal neurologic symptoms, visual disturbances, loss of bladder control, etc. However, acute onset symptoms may also be seen. MRI scans can accurately diagnose spinal or cerebral lesions and is also helpful in monitoring progress while on treatment. Albendazole is currently the drug of choice along with steroids for medical management of neurocysticercosis. The case of intramedullary spinal neurocysticercosis was treated with praziquantel.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
661
664
https://jesp.journals.ekb.eg/article_90156_ef46dadb933c3526250b290ee3fb1a1f.pdf
dx.doi.org/10.21608/jesp.2014.90156
INSECTS ASSOCIATED WITH HOSPITAL ENVIRONMENT IN EGYPT WITH SPECIAL REFERENCE TO THE MEDICALLY IMPORTANT SPECIES
MOHAMED
KENAWY
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia,
Cairo 11566, Egypt.
author
HANAN
AMER
Infection Control Unit, Ain Shams University Specialized Hospital,
Abbassia, Cairo 11566, Egypt.
author
NADIA
LOTFY
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia,
Cairo 11566, Egypt.
author
NAGWA
KHAMIS
Infection Control Unit, Ain Shams University Specialized Hospital,
Abbassia, Cairo 11566, Egypt.
author
YOUSRYA
ABDEL-HAMID
Research Institute of Medical Entomology, The General
Organization for Institutes and Teaching Hospitals, Ministry of Health, Dokki, Giza
12311, Egypt.
author
text
article
2014
eng
A study was planned to examine the insect fauna associated with two hospitals: urban (A) in Cairo and rural (B) in Banha, Egypt with varying hygienic levels and their adjacent residential areas (AC) and (BC), respectively and to investigate the effect of hygienic level on species composition and relative abundance. A total of 22 species belonging to 7 orders and 15families were reported in the four study areas of which, Dipterous flies were the most common (8/22, 36.36% species). A total of 5257 adults were collected of which Dipterous flies were the abundant (3800, 72.28% insect) and Musca domestica was the most abundant species (3535, 67.24% insect) which was present in all areas where it was more common / predominant species (21.94%-90.91% insect). Moreover, higher densities of M. domestica were in (B) and BC than in (A) or (AC). The heavily infested area was AC (54.55% species) followed by (A), (BC) and (B) however, the total number of the collected insects was higher in (BC) and (B) than in (AC) and (A). This was confirmed by finding maximum diversity indices in (AC) and minimum ones in B. In all areas, means of M. domestica was more common during summer/ autumn and spring than in the winter. Periplaneta americana collected only during autumn in AC and was more common in autumn in (BC) while Blatella germanica collected only during summer in (AC) and was more common in autumn in (B). The prevalence and higher abundance of the medically important species mainly M. domestica, P. americana and B. germanica in rural hospital than in urban one attribute mainly to the lower hygienic level of rural hospital This require a control program based mainly on sanitation supplemented by other measures to overcome the risk of disease transmission by such insects.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
665
676
https://jesp.journals.ekb.eg/article_90157_e8f1818c1586b08a70e7f88b9f460a05.pdf
dx.doi.org/10.21608/jesp.2014.90157
EVALUATION OF RIFAXIMIN IN MANAGEMENT OF HEPATIC ENCEPHALOPATHY
ALI
WAHIB
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
M.
SALEM
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
MOSTAFA
AHMED
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
YASSER
EL-DESSOUKY
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
DIAA
EL-TIBY
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
KHALED
EL-MOLA
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Nasr City, Egypt.
author
ABEER
EL-SAYED
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University,
Giza, Egypt.
author
text
article
2014
eng
This study evaluated the efficacy of rifaximin versus lactulose in the treatment of hepatic encephalopathy (HE). The study population included 50 patients who were diagnosed to have signs of the first to third degree HE, according to the West Haven criteria and classified into two groups. GI: included 25 patients who had HE and were treated with lactulose syrup (laxolac) 90 ml daily divided into 3 doses for 7 days. GII: included 25 patients who had HE and were treated with rifaximin 1200 mg daily divided into 3 doses for 7 days. Patients in both groups also received other measures of treatment of HE as daily enema and protein restriction. Rifaximin significantly improved various subjective and measurable components of HE including mental status, behavior, asterixis, and serum ammonia concentration. In acute HE of grade 1 to grade 3, rifaximin may be a good alternative to non-absorbable disaccharides as it is broad spectrum, non-absorbable antibiotic with rapid action and little side effects. It is better tolerated, associated with less frequent and shorter hospitalization in comparison to lactulose.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
677
685
https://jesp.journals.ekb.eg/article_90158_dc6dd83fd47743e5c9668ca36be2f47e.pdf
dx.doi.org/10.21608/jesp.2014.90158
EFFECT OF DEXEMEDITOMEDINE AND PROPOFOL ON THE PREVENTION OF EMERGENCE AGITATION FOLLOWING SEVOFLURANE ANESTHESIA IN EGYPTIAN CHILDREN
MOFEED
ABDEL-MA’BOUD
Department of Anesthesia and ICU, Faculty of Medicine, Al-Azhar University,
Nasr City, Cairo, Egypt.
author
text
article
2014
eng
This study evaluated the effect of dexemeditomedine and propofol on the prevention of emergence agitation following sevoflurane anesthesia in children. Sixty children, aged 4-6 years, ASA I, who were undergoing inguinal hernia repair were enrolled after written informed consents were obtained from parents of all participants. The results showed that the incidence of EA showed statistical significant reduction in dexemedetomedine group (15%) than propofol group (20%) and much reduction than saline group (60%) with no difference in the discharge time, FLACC scale was clinically but not statistically lower in dexemedetomedine group than propofol group and much lower than saline group at all times with no significant difference in the number of patients administered fentanyl, Ramsay's sedation scale was clinically higher in dexemeditomedine group than propofol group and much higher than saline group at all times but without statistical difference, inspired sevoflurane concentration was statistically lower in dexemeditomedine group than the other two groups at all times except at T7, where it was reduced in both dexemeditomedine and propofol groups but the reduction was more in dexemeditomedine group and heart rate (HR) and mean arterial pressure (MAP) showed statistical significant reduction in dexemedetomedine group than the other two groups at all times compared to baseline value (T0) except at T7, where they were reduced in both dexemedetomedine and propofol groups. In dexemedetomedine group, eight cases (40%) showed reduction in HR and four cases (20%) showed reduction in MAP but only one case (5%) required atropine and other one (5%) required ephedrine while in propofol group, one case (5%) showed reduction in HR and other case (5%) showed reduction in MAP but did not require atropine or ephedrine respectively.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
687
694
https://jesp.journals.ekb.eg/article_90159_0e0a1e616053399266429a653dde36b3.pdf
dx.doi.org/10.21608/jesp.2014.90159
ASSESSMENT OF DIAGNOSTIC AND THERAPEUTIC APPROACHES OF HELICOBACTER PYLORI-ASSOCIATED IRON DEFICIENCY AND ANEMIA IN CHILDREN WITH DYSPEPTIC SYMPTOMS
MOHIEE EL-DEEN
AWAD
Department of Pediatrics, Faculty of Medicine,
Tanta University, Tanta, Egypt.
author
SALEH
AMIN
Department of Pediatrics, Faculty of Medicine,
Tanta University, Tanta, Egypt.
author
SAIED
ABDOU
Department of Clinical Pathology, Faculty of Medicine,
Tanta University, Tanta, Egypt.
author
text
article
2014
eng
This study assessed the diagnostic approaches of Helicobacter pylori (HP)-associated iron deficiency (ID) and anemia (IDA) in children with dyspeptic symptoms and evaluated the effects of simultaneous anti-H. pylori (anti-HP) therapy and oral iron in comparison with each of anti- HP therapy and oral iron therapy alone, on iron status as assessed by serum soluble transferrin receptor (sTfR) level. Two hundreds children with dyspeptic symptoms were subjected to clinical evaluation, stool examination, CBC, biochemical assays for serum iron parameters and measurements of serum IgG antibodies to HP and serum sTfR level by ELISA. Sixty children were found to have HPassociated ID or IDA and were randomly divided into 3 groups (20 children each). GA received 2-week anti-HP therapy plus 90-day oral iron, and GB received 2-week anti-HP therapy alone whereas group C received 90-day oral iron alone. Re-evaluation of the 3 groups was performed after 3 months of treatment initiation by repeat CBC and serum sTfR level. Children (45%) were HP-seropositive. The mean values of serum sTfR were significantly higher in HP-positive group and in HP-positive children with IDA than in HP-negative group and in HP-negative children with IDA although no significant differences were noted in hematologic variables and iron parameters between the corresponding groups and children. As regard treatment groups, there were significant improvements in the mean values of indices of IDA status (Hb, MCH, MCV, sTfR) and ID status (sTfR) at 3 months of treatment initiation compared with their baseline values after anti-HP triple therapy either with oral iron or without oral iron whereas the control children who were treated with oral iron alone showed insignificant changesdespite oral iron administration. The improvements in these parameters were significantly greater in groups of children who received anti-HP therapy either combined with iron or alone when compared with those who did not receive anti-HP therapy. Their magnitudes were significantly higher among children receiving anti-HP therapy combined with oral iron when compared with that receiving anti-HP therapy alone.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
695
708
https://jesp.journals.ekb.eg/article_90160_e7bfa68fe832148dc2d0cf2530fea45c.pdf
dx.doi.org/10.21608/jesp.2014.90160
SEROLOGICAL VERSUS ANTIGEN DETECTION METHODS FOR GIARDIA DUODENALIS DIAGNOSIS
M.
BASHIR
Departments of Environmental Research, Theodor
Bilharz Research Institute, Giza, Egypt.
author
A.
FARID
Department of Zoology,
Faculty of Science, Cairo University, Egypt.
author
I.
RABIA
Departments of Environmental Theodor
Bilharz Research Institute, Giza, Egypt.
author
B.
MOSTAFA
Departments of Environmental Research, Theodor
Bilharz Research Institute, Giza, Egypt.
author
A.
EL AMIR
Department of Zoology,
Faculty of Science, Cairo University, Egypt.
author
text
article
2014
eng
Giardiasis constitutes an important public health problem in the world. Contamination of the water with fecal materials including viruses and pathogenic protozoa still represents an environmental health hazard, especially in rural areas. The survey study evaluated the relation between seropositivity and some risk factors. Moreover, the study compared between the serological IgG and IgM level and antigen detection methods for the diagnosis of giardiasis. The results indicate that sex distribution and age were the mean risk factors for seroprevelence. In this study, sera samples were employedin sandwich ELISA assay, to detect circulating Giardia antigens. None of the negative control serum samples gave a positive reaction, but cross reaction was encountered with 3 case of Cryptosporidium. The specificity of the assay was 94.83%. On the other hand, the sensitivity of the Giardia patient’s sera was 94.12% which was higher than that of IgG (86.25%) and IgM (87.50%) secretion measurements. In conclusion, antigen detection methods give better and earlier diagnosis for giardiasis can be performed quickly and do not require an experienced and skilled morphologist.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
709
718
https://jesp.journals.ekb.eg/article_90161_eb2d4c644df3d3a548116ebecc2c7829.pdf
dx.doi.org/10.21608/jesp.2014.90161
SUPERIORITY OF FLOURESCENT IN SITU HYBRIDIZATION OVER IMMUNOHISTOCHEMISTRY IN DETECTION OF HER2 GENE IN CARCINOMA OF THE URINARY BLADDER ASSOCIATED WITH AND WITHOUT SCHISTOSOMIASIS
OLFAT
HAMMAM
Department of Pathology, Theodor Bilharz Research Institute,
Imbaba, Giza, Egypt.
author
MOHAMED
WISHAHI
Department of Urology, Theodor Bilharz Research Institute,
Imbaba, Giza, Egypt.
author
ALI
HINDAWI
Department of Pathology, Faculty of Medicine,
Cairo University, Cairo, Egypt.
author
MAHA
MOSAAD
Department of Pathology, Faculty of Medicine,
Cairo University, Cairo, Egypt.
author
MAHA
AKL
Department of Pathology, Theodor Bilharz Research Institute,
Imbaba, Giza, Egypt.
author
HEBA
KHALIL
Department of Pathology, Theodor Bilharz Research Institute,
Imbaba, Giza, Egypt.
author
HOSSAM
AL GANZOURY
Department of Pathology, Faculty of Medicine,
Cairo University, Cairo, Egypt.
author
MOHAMED
BADAWY
Department of Pathology, Faculty of Medicine,
Cairo University, Cairo, Egypt.
author
KHALED
ELESAILY
Department of Pathology, Faculty of Medicine,
Cairo University, Cairo, Egypt.
author
text
article
2014
eng
HER2 is an oncogene encoding a type 1 tyrosine kinase growth factor receptor and the role of HER2 in the development of numerous types of human cancer is still understood and correlates with clinical outcome, poor prognosis, it is a predictor factor for poor response to chemotherapy. HER2 overexpression is associated with reduced disease free and overall survival. Patients who have HER2 negative expression have a poor prognosis. The aim of the present study is to explore the accuracy of detection of expression of HER2 protein by two different techniques of immunohistochemistry (IHC) and gene amplification by fluorescent in situ hybridization (FISH). The two techniques were applied to sixty two patients that included different cell types of carcinoma of the bladder, benign bilharzial lesions and control. Characteristics of the 62 patients are: 10 chronic cystitis, 19 squamous cell carcinoma (SCC) with schistosomiasis, 33 urothelial carcinoma (UC) schistosomal and non-schistosomal, ten healthy individuals without schistosomiasis served as controls. Gene amplification of HER2 was done using FISH and protein expression of HER2 by IHC. The study was applied on archival data of formalin-fixed paraffin embedded tissues and patient clinical data and follow up for 5 years. Overexpression of HER2 protein was found in 30/52 (57.7%). Fourteen cases had score of 2+, and sixteen cases had score of 3+. Using FISH technique it showed more accurate detection of HER2 gene as those fourteen cases who had score of 2+ had been found to be 5 out of 14 were positive for gene over expression , the other sixteen who had score of 3+ all were positive for gene amplification. HER2 protein and gene was found to be significantly overexpressed in carcinoma of the bladder in both cell types SCC and UC with or without schistosomiasis compared to the benign lesions and control groups (P <0.01) by both techniques. There is significant increase in expression of HER2 protein and gene in SCC compared to UC (P< 0.01). In UC overexpression of HER2 protein and gene was evident in all stages Ta, T1, T2-4. HER2 protein and gene overexpressed in different grades of UC. In SCC HER2 protein and gene had overexpression in different stages and grades.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
719
731
https://jesp.journals.ekb.eg/article_90367_b7d8e47006b4082a2e6065c0a2343986.pdf
dx.doi.org/10.21608/jesp.2014.90367
EXPRESSION OF CYTOKERATIN 7, 20, 14 IN UROTHELIAL CARCINOMA AND SQUAMOUS CELL CARCINOMA OF THE EGYPRIAN URINARY BLADDER CANCER
OLFAT
HAMMAM
Department of Pathology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
MOHAMED
WISHAHI
Department of Urology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt
author
HEBA
KHALIL
Department of Pathology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
HOSSAM
EL GANZOURI
Department of Urology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
MOHAMED
BADAWY
Department of Urology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
AMR
ELKHOULY
Department of Urology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
KHALED
ELESAILY
Department of Urology, Theodor Bilharz Research Institute,
Imbaba P.O. Box 30, Giza, Egypt.
author
text
article
2014
eng
This study estimated the expression of CK-7, CK14, and CK-20 protein in human bladder carcinoma, urothelial carcinoma (UC) in comparison to squamous cell carcinoma (SCC) and to show its possible correlation to clinicopathologic parameters (grade and stage and bilharziasis), and investigate whether cytokeratin 14 immunostaining may be useful to detect earlysquamous metaplasia in bladder biopsies and in association with UC. We evaluated the bladder tissues of 200 patients with bladder carcinoma, 150 patients had UC, and 50 patients had SCC. Imunohistochemical technique was used for detection of CK7, CK14 and CK20 monoclonal antibodies. The mean age of the patients was 55 years (range51- 70 years). The UC were classified according to grades into grade I, II and III in 20, 40 and 90 cases, respectively. Stages of UC were: Ta in 10, T1 in 60 and 90 patients with muscle-invasive T2-3. In UC cases 105 /150 (70%) were positive for over expression of CK20. In the same group of UC 120/150 (80) were positive for over expression of CK7. Negative expression was found in SCC cases. A High grades of the UC were associated with decrease expression of CK 20, there were 20 (100%) in GI, 35 (87.5%) in GII, 50 (68.6%) in GIII (P <0.01), and an increase expression of CK7 4 (20%) in GI, 26 (65%) in GII, 90(100%) in GIII (P <0.01). CK20 expression decreased as the tumor stages increased, it was 15 (100%) in Ta, 50 (83.3%) in T1, 40 (50%) in T2-3 (P <0.01), while CK7 showed increase expression in 2 cases with Ta tumor(20%), 38 (47.5%) in T1, 80 (100%) in T2-T3 (P <0.01). The present study confirmed that CK14 is expressed in SCC and in UC with squamous differentiation.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
733
740
https://jesp.journals.ekb.eg/article_90369_4ad2d88e8a0bfe0e19eb68caf7311950.pdf
dx.doi.org/10.21608/jesp.2014.90369
AFRICAN TRYPANOSOMIASIS WITH SPECIAL REFERENCE TO EGYPTIAN TRYPANOSOMA EVANSI: IS IT A NEGLECTED ZOONOSIS?
MAMDOUH
EL-BAHNASAWY
Military Medical Academy, Egypt.
author
MAI
KHATER
Military Medical Academy, Egypt.
author
TOSSON
MORSY
Department of Parasitology, Faculty of Medicine,
Ain Shams University, Cairo 11566, Egypt.
author
text
article
2014
eng
Trypanosomes (including humans) are blood and sometimes tissue parasites of the order Kinetoplastida, family Trypanosomatidae, genus Trypanosoma, principally transmitted by biting insects where most of them undergo a biological cycle. They are divided into Stercoraria with the posterior station inoculation, including T. cruzi, both an extra- and intracellular parasite that causes Chagas disease, a major human disease affecting 15 million people and threatening 100million people in Latin America, and the Salivaria with the anterior station inoculation, mainly African livestock pathogenic trypanosomes, including the agents of sleeping sickness, a major human disease affecting around half a million people and threatening 60 million people in Africa. Now, T. evansi was reported in man is it required to investigate its zoonotic potential?
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
741
748
https://jesp.journals.ekb.eg/article_90371_d8035458a11367a219de00af2f56a400.pdf
dx.doi.org/10.21608/jesp.2014.90371
AIR QUALITY IN AIN SHAMS UNIVERSITY SURGERY HOSPITAL
M.
EL AWADY
Department of Community, Environmental, and Occupational Medicine, Egypt.
author
A.
ABD EL RAHMAN
Department of Microbiology and Immunology, Faculty of Medicine,
Ain Shams University, Cairo 11566, Egypt.
author
L.
AL BAGOURY
Department of Community, Environmental, and Occupational Medicine, Egypt.
author
I.
MOSSAD
Department of Community, Environmental, and Occupational Medicine, Egypt.
author
text
article
2014
eng
Through air sampling, it was possible to evaluate microbial contamination in environments at high risk of infection, and to check the efficiency of ventilation system and the medical team’s hygiene procedures. This study measured the concentration of particulate matter (PM) 2.5 or less microns and microbiological organisms in operating rooms (OR),intensive care units (ICU) and emergency rooms (ER) in Ain Shams University Surgery Hospital, and to assess ventilation characteristics in operating rooms in the hospital. The passive air sampling was done from ICUs, ORs, and ERs in Ain Shams University Surgery Hospital. Also for each operating room, an observational checklist was done to record other factors that may affect air quality in the room. The evaluated air quality indices were: suspended (PM) 2.5 micrometer or less, culture media and microbial identification of bacteria and fungi, and temperature and relative humidity. The results showed that the highest mean found for bacterial (105.70±30.49) and fungi concentration (7.50±5.30) was in ER. The three settings did not differ statistically as regard levels of PM 2.5, temperature, and relative humidity. A positive correlation exits between bacteria and fungi concentration on one hand and relative humidity on the other. Diphteroid, CONS, MRSA, S. aureus, and Anthracoid were the most frequent isolated bacterial types, while Penicillium and Asperigillus fumigatus were the most frequent isolated fungi. In operating rooms, the percent of unmasked persons present and the temperature positively influence the bacterial count, while ventilation condition is negatively influencing fungi count, and the number of persons present in the operating room positively affects the PM level.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
749
759
https://jesp.journals.ekb.eg/article_90373_6eaf6c4dfc9eba3106c7d4fb2e4f7dc3.pdf
dx.doi.org/10.21608/jesp.2014.90373
COMPARATIVE FINE STRUCTURE OF EGGS OF AUTOGENOUS AND ANAUTOGENOUS CULEX PIPIENS (DIPTERA: CULICIDAE)
BELAL
SOLIMAN
Department of Zoology, Faculty of Science, Suez University, Suez, Egypt.
author
MAHA
TEWFICK
Department of Zoology, Faculty of Science, Suez University, Suez, Egypt.
author
NAHLA
WASSIM
Department of Zoology, Faculty of Science, Suez University, Suez, Egypt.
author
text
article
2014
eng
Culex (Cx.) pipiens is the potential vector of human filariasis in Egypt. However, autoge- nous Cx. pipiens may be less efficient vector of Wuchereria (W.) bancrofti in endemic areas of Egypt compared to anautogenous counterparts. In this study, an attempt was made to differentiate eggs of autogenous and anautogenous Cx. pipiens using scanning electron micrographs. The results indicated that eggs of both species appear to be similar to great extent in surface morphology. Eggs of both forms are black and elongate-oval. Width is greatest at the anterior end. The posterior end is pointed. Themicropylar disc is apparent with distinct edge. Exochorionic bridges are angular. Size of both eggs represented by length and width are comparable. In both eggs, length is greater than width. However, eggs of both forms can be distinguished from each other by the exochorionic bridges being longer and thinner in the autogenous eggs than in the anautogenous eggs.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
761
769
https://jesp.journals.ekb.eg/article_90375_1f702e6e0e34ca6444f7d2efab5b920b.pdf
dx.doi.org/10.21608/jesp.2014.90375
EVALUATION OF SOME MEDICINAL PLANTS IN CONTROLLING CULEX PIPIENS
ESSAM
ABDEL-SATTAR
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo11562,
Egypt.
author
AHMED
ZAITOON
Environment and Arid Land Agriculture, Faculty
of Metrology, King Abdul-Aziz University, Jeddah, Saudi Arabia.
author
ABEER
EL SAYED
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo11562,
Egypt.
author
A.
BAKHASHWAIN
Environment and Arid Land Agriculture, Faculty
of Metrology, King Abdul-Aziz University, Jeddah, Saudi Arabia.
author
text
article
2014
eng
The study evaluated the efficacy of methanolic extracts of Ruta chalepensis (Rutaceae), Withania somnifera (Solanaceae), Cleome paradoxa (Capparaceae) and Heliotropium longiflorum (Boraginaceae) aerial parts against Culex pipiens larvae. Different concentrations (100-500ppm) of the methanolic extracts of the plants were tested towards larval mortality and development of C. pipiens separately. Larval mortalities were counted at 2, 4 & 10 days after treatment. Egg hatchability was determined at 4 & 7 days after treatment. Successful pupation and adult emergence percentage were recorded daily till all control adults emerged. All plants extracts exhibited variable activities. The greatest effect was with R. chalepensiswhich showed acute (2 days) and chronic (10 days) LC50 of 132.6 & 96.56 ppm, respectively. Larval mortality up to 84.47% &85.53%, were observed with C. paradoxa and R. Chalepensis respectively. Egg hatch was significantly reduced about equal with R. chalepensis and W. somnifera extracts. Concentration levels of C. paradoxa (≥200 ppm) and H. longiflorum (≥400ppm) showed significant hindrance to the larval development and reduction to resulting pupae and adults. Drastic development retardation was shown with extract of C. paradoxa leaves (300ppm), but only 15.3% & 5.6% of larvae reach pupal and adult stages respectively. The larvicidal activity of methanolic extracts of R. chalepensis, W. somnifera, C. paradoxa, H. longiflorum proved to be effective against C. pipiens larvae without any human or animal risk.
Journal of the Egyptian Society of Parasitology
The Egyptian Society of Parasitology
1110-0583
44
v.
3
no.
2014
771
778
https://jesp.journals.ekb.eg/article_90376_7a607677101e88a85514876e5dfcce88.pdf
dx.doi.org/10.21608/jesp.2014.90376